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PC12 Cell Protective Effects of Broccoli (Brassica oleracea var. italica) Leaf Fraction against H2O2-induced Oxidative Stress

H2O2로 유발된 산화적 스트레스에 대한 브로콜리(Brassica oleracea var. italica) 잎 분획물의 PC12 cell 보호 효과

  • Park, Seon Kyeong (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Jin, Dong Eun (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Park, Chang Hyeon (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Seung, Tae Wan (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Choi, Sung-Gil (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Heo, Ho Jin (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University)
  • 박선경 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 진동은 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 박창현 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 승태완 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 최성길 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 허호진 (경상대학교 응용생명과학부, 농업생명과학연구원)
  • Received : 2014.04.03
  • Accepted : 2013.05.26
  • Published : 2014.08.31

Abstract

To examine the physiological effects of broccoli (Brassica oleracea var. italica) leaf, the bioavailable compounds in broccoli leaf extract, and its in vitro neuroprotective effects against $H_2O_2$-induced oxidative stress were examined in this study. The chloroform fraction of broccoli leaf extract had the highest total phenolic content of all the fraction than others, and the highest 2,2"-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical-scavenging activity and malondialdehyde (MDA) inhibitory effect. Intracellular reactive oxygen species (ROS) accumulation resulting in $H_2O_2$-treated in PC12 cells was significantly lower when the chloroform fraction was present in the medium compared to that in PC12 cells treated with $H_2O_2$ alone. In a cell viability assay performed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), the chloroform fraction showed protective effects against $H_2O_2$-induced neurotoxicity and inhibited lactate dehydrogenase (LDH) release into the medium. High-performance liquid chromatography (HPLC) analysis showed that ferulic acid was the predominant phenolic compound in chloroform fraction of broccoli leaf.

Keywords

broccoli;Brassica oleracea var. italica;PC12 cell;ferulic acid

Acknowledgement

Supported by : 한국연구재단

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