DOI QR코드

DOI QR Code

Effects of Unripe Black Raspberry Water Extract on Lipid Metabolism and Oxidative Stress in Mice

복분자 미숙과 물 추출물이 마우스의 지질대사 및 산화적 스트레스에 미치는 영향

  • Received : 2014.04.07
  • Accepted : 2014.05.17
  • Published : 2014.08.31

Abstract

We examined the effects of unripe black raspberry water extract (UBR-W) on lipid metabolism and oxidative stress in mice. C57BL/6J mice were divided into 4 groups: those administered a control diet (CTL), high-fat diet (HFD), UBR-W and simvastatin for 12 weeks. In the HFD group, LDL cholesterol were significantly higher than in the CTL group. However, the UBR-W treated group showed dose-dependent reduction of plasma LDL levels. Hepatic total lipid, TC, and malondialdehyde were significantly increased in hyperlipidemic mice. However, supplementation with either UBR-W or simvastatin effectively reduced these lipid profiles and lipid peroxidation. UBR-W increased mRNA expression of the LDL receptor, sterol regulatory element binding protein 2 (SREBP2), 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase and ATP-binding cassette transporter A1 (ABCA1) compared to that observed in the HFD group. In addition, UBR-W and simvastatin showed significantly reduced oxidized LDL uptake by the scavenger receptor CD36. These results suggest that UBR-W is useful for treatment and prevention of hyperlipidemia and lipid peroxidation.

Keywords

Unripe black raspberry;High-fat diet;Hyperlipidemia;Lipid metabolism;Oxidative stress

References

  1. Wang SY, Jiao H. Scavenging capacity of berry crops on superoxide radicals, hydrogen peroxide, hydroxyl radicals, and singlet oxygen. J. Agr. Food Chem. 48: 5677-5684 (2000) https://doi.org/10.1021/jf000766i
  2. Ministry of Health and Welfare. The third Korea national health and nutrition examination survey; health examination. Ministry of Health and Welfare, Sejong, Korea. pp. 36-49 (2005)
  3. Seeram NP, Adams LS, Zhang Y, Lee R, Sand D, Scheuller HS. Heber D. Blackberry, black raspberry, blueberry, cranberry, red raspberry, and strawberry extracts inhibit growth and stimulated apoptosis of human cancer cells in vitro. J. Agr. Food Chem. 54: 9329-9339 (2006) https://doi.org/10.1021/jf061750g
  4. Chen T, Hwang HJ, Rose ME, Nines RG, Stoner GD. Chemopreventive properties of black raspberries in N-nitrosomethylbenzylamine-induced rat esophageal tumorigenesis: Down regulation of cyclooxygenase-2, inducible nitric oxide synthase, and c-jun. Cancer Res. 66: 2853-2859 (2006) https://doi.org/10.1158/0008-5472.CAN-05-3279
  5. Jonasson L, Holm J, Skalli O, Bondjers G, Hansson GK. Regional accumulations of T cells, macrophages, and smooth muscle cells in the human atherosclerosis plaque. Arteriosclerosis. 6: 131-138 (1986) https://doi.org/10.1161/01.ATV.6.2.131
  6. Hsu HY, Nicholson AC, Hajjar DP. Inhibition of macrophage scavenger receptor activity by tumor necrosis factor-${\alpha}$ is transcriptionall and post trascriptionally regulated. J. Biol. Chem. 271: 7767-7773 (1996) https://doi.org/10.1074/jbc.271.13.7767
  7. Kwon JW, Lee HK, Park HJ, Kwon TO, Choi HR, Song JY. Screening of biological activities to different ethanol extracts of Rubus coreanus miq. Korean J. Med. Sci. 19: 325-333 (2011) https://doi.org/10.7783/KJMCS.2011.19.5.325
  8. MolcnyiovA, StanckovA, JavorskM, Tkc I. Beneficial effect of simvastatin treatment on LDL oxidation and antioxidant protection is more pronounced in combined hyperlipidemia than in hypercholesterolemia. Pharmacol. Res. 54: 203-207 (2006) https://doi.org/10.1016/j.phrs.2006.04.009
  9. Yang HM, Oh SM, Lim SS, Shin HK, Oh YS, Kim JK. Antiinflammatory activities of Rubus coreanus depend on the degree of fruit ripening. Phytother. Res. 22: 102-107 (2011)
  10. Lee SJ, Lee MJ, Ko YJ, Choi HR, Jeong JT, Choi KM, Cha JD, Hwang SM, Jung HK, Park JH, Lee TB. Effects of extracts of unripe black raspberry and red ginseng on cholesterol synthesis. Korean J. Food Sci. Technol. 45: 628-635 (2013) https://doi.org/10.9721/KJFST.2013.45.5.628
  11. Choi HR, Lee SJ, Lee JH, Kwon JW, Lee HK, Jeong JT, Lee TB. Cholesterol-lowering effects of unripe black raspberry water extract. J. Koean Soc. Food Sci. Nutr. 42: 1899-1907 (2013) https://doi.org/10.3746/jkfn.2013.42.12.1899
  12. Song G1, Liu J, Zhao Z, Yu Y, Tian H, Yao S, Li G, Qin S. Simvastatin reduces atherogenesis and promotes the expression of hepatic genes associated with reverse cholesterol transport in apoE-knockout mice fed high-fat diet. Lipids in Health and Disease 10: 8 (2011) https://doi.org/10.1186/1476-511X-10-8
  13. Zhang X, Mao S, Luo G, Wei J, Berggren-Sderlund M, Nilsson-Ehle P, Xu N. Effects of simvastatin on apolipoprotein M in vivo and in vitro. Lipids Health Dis. 10: 112 (2011) https://doi.org/10.1186/1476-511X-10-112
  14. Lei YF, Chen JL, Wei H, Xiong CM, Zhang YH, Ruan JL. Hypolipidemic and anti-inflammatory properties of Abacopterin A from Abacopteris penangiana in high-fat diet-induced hyperlipidemia mice. Food Chem. Toxicol. 49: 3206-3210 (2011) https://doi.org/10.1016/j.fct.2011.08.027
  15. Okawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbitruic acid reaction. Anal. Biochem. 95: 351-358 (1979) https://doi.org/10.1016/0003-2697(79)90738-3
  16. Ghasi S, Nwobodo E, Ofili Jo. Hypocholesterolemic effects of crude extract of leaf of Moringa oleifera Lam in high fat diet fed wistar rats. J. Ethnophamacol. 69: 21-25 (2000) https://doi.org/10.1016/S0378-8741(99)00106-3
  17. Neuschwander-Tetri BA, Clark JM, Bass NM, Van Natta ML, Unalp-Arida A, Tonascia J, Zein CO, Brunt EM, Kleiner DE, McCullough AJ, Sanyal AJ, Diehl AM, Lavine JE, Chalasani N, Kowdley KV, NASH Clinical Research Network. Clinical, laboratory and histological associations in adults with non-alcoholic fatty liver disease. Hepatology. 52: 913-924 (2010).
  18. Ferreira de Araujo PR, da Silva Santos V, Rodrigues Machado A, Gevehr Fernandes C, Silva JA, da Silva Rodrigues R. Benefits of blackberry nectar (Rubus spp.) relative to hypercholesterolemia and lipid peroxidation. Nutr. Hosp. 26: 984-990 (2011)
  19. Kim SK, Kim CK, Lee KS, Kim JH, Hwang H, Jeoung D, Choe J, Wond MH, Lee H, Ha KS, Kwone YG, Kim YM. Aqueous extract of unripe Rubus coreanus fruit attenuates atherosclerosis by improving blood lipid profile and inhibiting NF-${\kappa}B$ acvication via phase II gene expression. J. Ethnopharmacol. 146: 515-524 (2013) https://doi.org/10.1016/j.jep.2013.01.016
  20. Joo IW, Ryu JH, Oh HJ. The influence if Sam-Chil-Geun (Panax notoginseng) on the serum lipid levels and inflammations of rats with hyperlipidemia induced by poloxamer-407. Yonsei Med. Jul. 51: 504-510 (2010) https://doi.org/10.3349/ymj.2010.51.4.504
  21. Bhandary B, Lee HY, Back HI, Park SH, Kim MG, Kwon JW, Song JY, Lee HK, Kim HR, Chae SW, and Chae HJ. Immature Rubus coreanus shows a free radical-scavenging effect and inhibits cholesterol synthesis and secretion in liver cells. Indian J. Pharm. Sci. 74: 211-216 (2012) https://doi.org/10.4103/0250-474X.106062
  22. Park SH, Kim JL, Lee ES, Han SY, Gong JH, Kang MK, Kang YH. Dietary ellagic acid attenuates oxidized LDL uptake and stimulates cholesterol efflux in murine macrophages1-3. J. Nutr. 141: 1931-1937 (2011) https://doi.org/10.3945/jn.111.144816
  23. Goldstein JL, Brown MS. The LDL receptor. Arterioscler Thromb Vasc Biol. 29: 431-438 (2009) https://doi.org/10.1161/ATVBAHA.108.179564
  24. Weng YC, Chuang ST, Lin YC, Chuang CF, Chi TC, Chiu HL, Kuo YH, Su MJ. Caffeic acid phenylethyl amide protects against the metabolic consequences in diabetes mellitus induced by diet and streptozocin. Evid. Based Complement Alternat. Med. 984780 (2012)
  25. Meddings JB, Spady DK, Dietschy JM. Kinetic characteristics and mechanisms of regulation of receptor-dependent and receptor-independent LDL transport in the liver of different animal species and humans. Am. Heart J. 113: 475-481 (1987) https://doi.org/10.1016/0002-8703(87)90617-X
  26. Brown MS, Goldstein JL. A receptor-mediated pathway fir cholesterol homeostasis. Science. 232: 34-47 (1997)
  27. Taniguchi T, Chen J and AD. Cooper Regulation of cholesterol 7 ${\alpha}$-hydroxylase gene expression in Hep-G2 cells. J. Biol. Chem. 269: 10071-10078 (1994)
  28. Vijayakumar MV, Pandey V. Hypolipidemic effects of fenugreek seeds is mediated through inhibition of fat accumulation and upregulation of LDL receptor. Obesity (Silver Spring). 18: 667-674 (2010) https://doi.org/10.1038/oby.2009.337
  29. Tall AR, Wang N. Tangier disease as a test of the reverse cholesterol transport hypothesis. J. Clin. Invest. 106: 1205-1207 (2000) https://doi.org/10.1172/JCI11538
  30. Gallou. G, Ruelland A, Legras B, Maugendre D, Allanic H and Cloarec L. Plasma malondialdehyde in type 1 and type 2 diabetic patients. Clin. Chim. Acta. 214: 227-234 (1993) https://doi.org/10.1016/0009-8981(93)90114-J
  31. Yoon I, Cho JY, Kuk JH, Jang MY, Ahn TH and Park KH. Identification and activity of antioxidative compounds from Rubus coreanum fruit. Korean J. Food Sci. Technol. 34: 898-904 (2002)
  32. Seo JS, Choi YM, Lee SM, Kong SH, Lee JS. Antioxidant activities and antioxidant compounds of some specialty rices. .J Korean Soc. Food Sci. Nutr. 37: 129-135 (2008) https://doi.org/10.3746/jkfn.2008.37.2.129
  33. Kissebah AH, Vydelingum N, Murray R, Evans DJ, Hartz AJ, Kalkhoff RK, Adams PW. Relation of body fat distribution to metabolic complications of obesity. J. Clin. Endocrinol. Metab. 54: 254-260 (1982) https://doi.org/10.1210/jcem-54-2-254
  34. Lee SE, Sung JS, Jang IB, Kim GS, Ahn TJ, Han HS, Kim JE, Kim YO, Park CB, Cha SW, Ahn YS, Park HK, Bang JK, Seong NS. Investigation on antioxidant activity in plant resources. Korean J. Medicinal Crop Sci. 16: 356-370 (2008)
  35. Han J, Hajjar DP, Febbraio M, Nicholson AC. Native and modified low density lipoproteins increase the functional expression of the macrophage class B scavenger receptor, CD36. J. Biol. Chem. 272: 21654-21659 (1997) https://doi.org/10.1074/jbc.272.34.21654
  36. Kim JY, Nolte LA, Hansen PA, Han DH, Ferguson K, Thompson PA, Holloszy JO. High-fat diet-induced muscle insulin resistance: relationship to visceral fat mass. Am. J. Physiol, Regul. Integr. Comp. Physiol. 279: 2057-2065 (2000)
  37. Krotkiewski M, Bjorntorp P, Sjostrom L, Smith U. Impact of obesity on metabolism in men and women. Importance of regional adipose tissue distribution. J. Clin. Invest. 72: 1150-1162 (1983) https://doi.org/10.1172/JCI111040
  38. Kim WJ, Kim SJ, Hwang MH, Jung HS, Kim HS and Cho HC. Effect of short term Rubus coreanus miquel supplementation on body composition maximum oxygen intake and blood lipid profiles, lactate and ammonia of middle age women with obesity during exercise program. J. Korean Soc. Living Environ. Sys. 15: 650-658 (2008)
  39. Jeong MY, Kim HL, Park JB, An HJ, Kim SH, Kim SJ, So HS, Park R, Um JY and Hong SH. Rubi Fructus (Rubus coreanus) inhibits differentiation to adipocytes in 3T3-L1 cells. Evidence-Based Complementary and Alternative Medicine 10 (2013)

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