DOI QR코드

DOI QR Code

Effects of the addition of Hizikia fusiforme, Capsosiphon fulvescens, and Undaria pinnatifida sporophyll on antioxidant and inhibitory potential against enzymes related to type 2 diabetes of vegetable extract

혼합야채추출물의 항산화 및 항당뇨 효과 증진을 위한 톳, 매생이, 미역귀의 첨가효과

  • Tong, Tao (Department of Food Engineering, Mokpo National University) ;
  • Zhang, Chengmei (Department of Food Engineering, Mokpo National University) ;
  • Ko, Du-Ok (Department of Food Engineering, Mokpo National University) ;
  • Kim, Sup-Bo (Department of Food Engineering, Mokpo National University) ;
  • Jung, Kwang-Jin (Department of Food Engineering, Mokpo National University) ;
  • Kang, Seong-Gook (Department of Food Engineering, Mokpo National University)
  • Received : 2014.01.08
  • Accepted : 2014.06.05
  • Published : 2014.08.30

Abstract

This study was conducted to investigate the effect of the addition of Hizikia fusiforme, Capsosiphon fulvescens, and Undaria pinnatifida sporophyll on the antioxidant and inhibitory potentials against key enzymes related to type 2 diabetes of a commercial vegetable extract. The nutritional quality and mineral concentration of a vegetable extract with seaweeds added were also analyzed. The addition levels of seaweed did not influence the proximate composition, whereas the calcium, sodium, potassium, magnesium, and iron concentrations significantly increased at the 5% Hizikia fusiforme and Undaria pinnatifida sporophyll addition levels. The 20% Hizikia fusiforme addition level significantly increased the total phenolic content and reducing power by 47.08% and 16.82%. The hydroxyl radical scavenging ability of the vegetable extract was not strengthened with the addition of seaweeds. The DPPH radical scavenging activity at the 20% Hizikia fusiforme, Capsosiphon fulvescens, and Undaria pinnatifida sporophyll addition levels significantly increased by 27.47%, 22.25%, and 17.27%, respectively. The vegetable extract with seaweeds added showed higher-level ${\alpha}$-glucosidase inhibition activities, accompanied by relatively weaker ${\alpha}$-amylase inhibition activity. In particular, at the 5% Undaria pinnatifida sporophyll addition level, the ${\alpha}$-glucosidase activity was significantly inhibited by 98.26%. Overall, the results showed that the incorporation of seaweeds into a vegetable extract effectively increased the mineral concentration and improved the antioxidant and inhibitory abilities of the extract on key enzymes linked to type 2 diabetes.

Acknowledgement

Supported by : Mokpo National University

References

  1. Lee SH, Park HJ, Chun HK, Cho SY, Cho SM, Lillehoj HS (2006) Dietary phytic acid lowers the blood glucose level in diabetic KK mice. Nutr Res, 26, 474-479 https://doi.org/10.1016/j.nutres.2006.06.017
  2. Tundis R, Loizzo MR, Statti GA, Menichini F (2007) Inhibitory effects on the digestive enzyme ${\alpha}$-amylase of three Salsolaspecies (Chenopodiaceae) in vitro. Pharmazie, 62, 473-475
  3. McDougall GJ, Stewart D (2005) The inhibitory effects of berry polyphenols on digestive enzymes. Biofactors, 23, 189-195 https://doi.org/10.1002/biof.5520230403
  4. Ranilla LG, Kwon YI, Apostolidis E, Shetty K (2010) Phenolic compounds, antioxidant activity and in vitro inhibitory potential against key enzymes relevant for hyperglycemia and hypertension of commonly used medicinal plants, herbs and spices in Latin America. Bioresour Technol, 101, 4677-4678
  5. Dalar A, Konczak I (2013) Phenolic contents, antioxidant capacities and inhibitory activities against key metabolic syndrome relevant enzymes of herbal teas from Eastern Anatolia Ind Crop Prod, 44, 383-390 https://doi.org/10.1016/j.indcrop.2012.11.037
  6. Zhang L, Li JR, Hogan S, Chung H, Welbaum GE, Zhou K (2010) Inhibitory effect of raspberries on starch digestive enzyme and their antioxidant properties and phenolic composition. Food Chem, 119, 592-599 https://doi.org/10.1016/j.foodchem.2009.06.063
  7. Chung DH, Kim SH, Myung N, Cho KJ, Chang MJ (2012) The antihypertensive effect of ethyl acetate extract of radish leaves in spontaneously hypertensive rats. Nutr Res Pract, 6, 308-314 https://doi.org/10.4162/nrp.2012.6.4.308
  8. Roberts CK, Sindhu KK (2009) Oxidative stress and metabolic syndrome. Life Sci, 84, 705-712 https://doi.org/10.1016/j.lfs.2009.02.026
  9. Henriksen EJ, Diamond-Stanic MK, Marchionne EM (2011) Oxidative stress and the etiology of insulin resistance and type 2 diabetes. Free Radic Biol Med, 51, 993-999 https://doi.org/10.1016/j.freeradbiomed.2010.12.005
  10. Nishibori N, Sagara T, Hiroi T, Sawaguchi M, Itoh M, Her S, Morita K (2012) Protective effect of Undaria pinnatifida sporophyll extract on iron induced cytotoxicity and oxidative stress in PC12 neuronal cells. Phytopharmacology, 2, 271-284
  11. Lou ZX, Wang HX, Lv WP, Ma CY, Wang ZP, Chen SG (2010) Assessment of antibacterial activity of fractions from burdock leaf against food-related bacteria. Food Control, 21, 1272-1278 https://doi.org/10.1016/j.foodcont.2010.02.016
  12. Prakash S, Jha SK, Datta N (2004) Performance evaluation of blanched carrots dried by three different driers. J Food Eng, 62, 305-313 https://doi.org/10.1016/S0260-8774(03)00244-9
  13. Li XL, Zhang HB, Xu HB (2009) Analysis of chemical components of shiitake polysaccharides and its antifatigue effect under vibration. Int J Biol Macromol, 45, 377-380 https://doi.org/10.1016/j.ijbiomac.2009.07.005
  14. Mohamed S, Hashim SN, Rahman HA (2012) Seaweeds: A sustainable functional food for complementary and alternative therapy. Trends Food Sci Tech, 23, 83-96 https://doi.org/10.1016/j.tifs.2011.09.001
  15. Nwosu F, Morris J, Lund VA, Stewart D, Ross HA, McDougall GJ (2011) Anti-proliferative and potential anti-diabetic effects of phenolic-rich extracts from edible marine algae. Food Chem, 126, 1006-1012 https://doi.org/10.1016/j.foodchem.2010.11.111
  16. Ananthi S, Raghavendran HR, Sunil AG, Gayathri V, Ramakrishnan G, Vasanthi HR (2010) In vitro antioxidant and in vivo anti-inflammatory potential of crude polysaccharide from Turbinaria ornata (Marine Brown Alga). Food Chem Toxicol, 48, 187-92 https://doi.org/10.1016/j.fct.2009.09.036
  17. Yang XM, Yu W, Ou ZP, Ma HL, Liu WM, Ji XL (2009) Antioxidant and immunity activity of water extract and crude polysaccharide from Ficus carica L. Fruit. Plant Food Hum Nutr, 64, 167-173 https://doi.org/10.1007/s11130-009-0120-5
  18. Lordan S, Smyth TJ, Soler-Vila A, Stanton C, Ross RP (2013) The ${\alpha}$-amylaseand${alpha}$-glucosidase inhibitory effects of Irish seaweed extracts. Food Chem, 141, 2170-2176 https://doi.org/10.1016/j.foodchem.2013.04.123
  19. AOAC (1990) Official Methods of Analysis. 15th ed, Association Official Analytical Chemists, Washington, DC, USA
  20. Singleton VL, Rossi JA (1965) Colorimetry of total phenolic with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic, 16, 144-158
  21. Smirnoff N, Cumbes Q (1989) Hydroxyl radical scavenging activity of compatible solutes. Phytochem, 28, 1057-1060 https://doi.org/10.1016/0031-9422(89)80182-7
  22. Yen GC, Chen HY (1995) Antioxidant activity of various tea extracts in relation to their antimutagenicity. J Agr Food Chem, 43, 27-32 https://doi.org/10.1021/jf00049a007
  23. Son MJ, Son SJ, Le SP (2008) Physicochemical properties of carrot juice containing Phellinus linteus extract and beet extract fermented by Leuconostoc mesenteroides SM. J Korean Soc Food Sci Nutr, 37, 798-804 https://doi.org/10.3746/jkfn.2008.37.6.798
  24. Chung HJ, Park HN, Chu YR, Jeon IS, Kang YS (2010) Quality characteristics and antioxidant activity of syrup added with maca (Lepidium meyenii) extract. Korean J Food Preserv, 17, 236-242
  25. Krentz AJ, Bailey CJ (2005) Oral antidiabetic agents current role in type 2 diabetes mellitus. Drugs, 65, 385-411 https://doi.org/10.2165/00003495-200565030-00005

Cited by

  1. Comparative Studies of the in vitro Antioxidant Properties of 80% Ethanol or Water Extracts of Formica rufa L. Gastrodia elata Blume, Cnidium officinale Makino and Their's Mixture vol.34, pp.1, 2018, https://doi.org/10.9724/kfcs.2018.34.1.27