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Effect of an extract of Bauhinia variegata leaves on chronic arsenic intoxication in mice (Mus musculus): A preliminary study

Biswas, Surjyo Jyoti;Ghosh, Goutam

  • Received : 2014.02.10
  • Accepted : 2014.08.25
  • Published : 2014.08.31

Abstract

Ethanolic leaf extract of Bauhinia variegata has been tested for its possible antioxidant potentials against sodium arsenite induced toxicity in mice. Mice were randomized into two groups of five and fifty mice. Group I consisting of 5 mice without any treatment with food and water ad libitum which served as normal control. Group II mice were fed with sodium arsenite in drinking water at 100 ppm concentration for two monthsthen they were segregated into five groups which were treated differently. Group II a mice received only arsenic as sodium arsenite with drinking water, Group II b were fed chronically 1 : 20 alcohol to distilled water (vehicle), Group II c, d, e mice were orally fed 50 mg/kg, 150 mg/kg and 250 mg/kg of B. variegata leaf extract of once daily for 15 and 30 days respectively along with arsenic. Several toxicity marker enzymes such as gamma glutamyl transferase, lactate dehydrogenase, aspartate and alanine aminotransferase, acid and alkaline phosphatase, catalase and superoxide dismutase along with haematological variables such as glucose 6-phosphate dehydrogenase, creatinine, bilirubin, haemoglobin and sugar in different groups of treated and control mice were studied. Results obtained from the in vivo experiment revealed that administration of sodium arsenite caused a significant increase in some enzymes while decrease in some. A similar trend was also observed with haematological variables. In contrast B. variegata treatment at 150 mg/kg favourably modulated these alterations and maintained the antioxidant status than other two doses i.e. 50 mg/kg and 250 mg/kg thereby making it a good candidate to be used as supportive palliating measures in arsenic induced toxicity.

Keywords

Bauhinia variegata;biomarkers;sodium arsenite;free radical;antioxidant

References

  1. Acharyya SK, Chakraborty P, Lahiri S, Raymahashay BC, Guha S, Bhowmik A. Arsenic poisoning in the Ganges delta. Nature. 1999;401:545-546. https://doi.org/10.1038/44052
  2. Ali MS, Azhar I, Amtul Z, Ahmed VV, Usmangani K. Antimicrobial screening of Caesalpiniaceae. Fitoterapia 1999;70:299-304. https://doi.org/10.1016/S0367-326X(99)00015-5
  3. Belon P, Banerjee A, Roy Karmakar S, Biswas SJ, Chaki Choudhury S, Banerjee P, Das JK Pathak S, Guha B, Bhattacharjee N, Paul S, Khuda-Bukhsh AR. Homeopathic remedy for arsenic toxicity?: Evidence-based findings from a randomized placebo-controlled double blind human trial. Sci Total Environ. 2007;384:141-150. https://doi.org/10.1016/j.scitotenv.2007.06.001
  4. Bergmeyer HU, Brent E. Methods in Enzymatic Analysis. Vol. 2. Bergmeyer HU ed. (Verlag Chemie Weinhein, USA: Academic Press New York), pp. 735-760, 1974.
  5. Biswas SJ, Bhattacharjee N, Khuda Bukhsh AR. Efficacy of a plant extract (Chelidonium majus L) in combating induced hepatocarcinogenesis in mice. Food Chem Toxicol. 2008;46:1474-1487. https://doi.org/10.1016/j.fct.2007.12.009
  6. Biswas SJ, Ghosh G, Roy Karmakar S, Bandyopadhyay TK. Comparative efficacy of Bauhinia variegate and Emblica officinalis against sodium arsenite induced toxicity in Mus musculus. Forsch Komplementmed. 2013;20:55.
  7. Chakraborti D, Sengupta MK, Rahman MM, Ahamed S, Chowdhury UK, Hossain MA, Mukherjee SC, Pati S, Saha KC, Dutta RN, Quamruzzaman Q. Groundwater arsenic contamination and its health effects in the Ganga-Meghna-Brahmaputra plain. J Environ Monit. 2004;6:74N-83N. https://doi.org/10.1039/b406573p
  8. Biswas SJ, Khuda Bukhsh AR. Cytotoxic and genotoxic effects of the azo-dye p-dimethylaminoazobenzene in mice: a time-course study. Mutation Res. 2005;587:1-8. https://doi.org/10.1016/j.mrgentox.2005.06.011
  9. Bodakhe SH, Ram A. Hepatoprotective properties of Bauhinia variegata bark extract. Yakugaku Zasshi. 2007;127:1503-1507. https://doi.org/10.1248/yakushi.127.1503
  10. Buege, J.A., Aust, S. Microsomal lipid peroxidation. Methods Enzymol. 1978;52:302-310. https://doi.org/10.1016/S0076-6879(78)52032-6
  11. Chaturvedi P, Pipedi,Tshekiso M, Moseki B, Kwape TE. Hepatoprotective potentials of water extract of Bauhinia purpurea bark against alcohol induced toxicity. Scientific Res Essays. 2011;6:4347-4353. https://doi.org/10.5897/SRE10.825
  12. Cohen SM, Arnold LL, Uzvolgyi E, Cano M, John MS, Yamamoto S, Lu XF, Le XC. Possible role of dimethylarsinous acid in dimethylarsinic acid-induced urothelial toxicity and regeneration in the rat. Chem. Res. Chem Res Toxicol. 2002;15:1150-1157. https://doi.org/10.1021/tx020026z
  13. Garcia-Chavez E, Santamaria A, Diaz-Barriga F, Mandeville P, Juarez BI, Jimenez-Capdeville ME. Arsenite-induced formation of hydroxyl radical in the striatum of awake rats. Brain Res. 2003;976:82-89. https://doi.org/10.1016/S0006-8993(03)02697-0
  14. Gay RJ, Mccomb RB, Browers GN. Optimum reaction conditions for human lactate dehydrogenase isoenzymes as they affect total lactate dehydrogenase activity. Clin Chem. 1968;14:740-753.
  15. Guha Mazumder DN, Das Gupta J, Santra A, Pal A, Ghose A, Sarkar S, Chattopadhaya N, Chakraborti D. Non-cancer effects of chronic arsenicosis with special reference to liver damage. In Arsenic Exposure and Health Effects. Abernathy CO, Calderon RL, Chappell WR ed. (London, UK: Chapman & Hall), pp. 112-123, 1997.
  16. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with Folin-phenol reagent. J Biol Chem. 195;193 265-275.
  17. Gupta AK, Vidyapati TJ, Chauhan JS. Chemical examination of stem of Bauhinia variegata. Planta Medica. 1980;38:174-176. https://doi.org/10.1055/s-2008-1074857
  18. International Agency for Research on Cancer. Overall Evaluation of Carcinogenicity: An Update of IARC Monographs 1 to 42 (Suppl. 7). In IARC Monograph on the Evaluation of Carcinogenic Risk to Humans. (Lyon, France; WHO). p100, 1987.
  19. Kakkar P, Das B and Viswanathan PN. A modified spectroscopic assay of superoxide dismutase. Indian J Biochem. Biophys. 1984;2:130-132.
  20. Milton AH, Smith W, Rahman B, Hasan Z, Kulsum U, Dear K, taRakibuddin M, Ali A. Chronic arsenic exposure and adverse pregnancy outcomes in Bangladesh. Epidemiology. 2005;16:82-86. https://doi.org/10.1097/01.ede.0000147105.94041.e6
  21. Nandi D, Patra RC, Swarup D. Effect of cysteine, methionine, ascorbic acid and thiamine on arsenic-induced oxidative stress and biochemical alterations in rats. Toxicology. 2005;211:26-35. https://doi.org/10.1016/j.tox.2005.02.013
  22. Nandi D, Patra RC, Swarup D. Arsenic residues in hair samples from cattle in some arsenic affected areas of West Bengal, India. Bull Environ Contam Toxicol. 2005;75:251-256. https://doi.org/10.1007/s00128-005-0745-5
  23. National Research Council. Arsenic in Drinking Water. (Washington DC, USA: National Academy Press), 2001.
  24. Pani SR, Mishra S, Panda PK. Nephroprotective effect of Bauhinia variegata (Linn.) whole stem extract against cisplatin-induced nephropathy in rats. Indian J Pharmacol. 2011;43:200-202. https://doi.org/10.4103/0253-7613.77370
  25. Rahman W, Begum SJ. Flavonoids from the white flowers of Bauhinia variegata. Natur Wissenschaften. 1966;53:384-385.
  26. Rajkapoor B, Jayakar B, Murugesh N. Antitumour activity of Bauhinia variegata on Dalton's ascitic lymphoma. J Ethnopharmacol. 2003;89:107-109. https://doi.org/10.1016/S0378-8741(03)00264-2
  27. Sahu G, Gupta PK. A review on Bauhinia variegata Linn. Int Res J Pharmacy. 2012;3:48-51.
  28. Rajkapoor B, Jayakar B,Murugesh N, Sakthisekharan D. Chemoprevention and cytotoxic effect of Bauhinia variegata against N-nitrosodiethylamine induced liver tumors and human cancer cell lines. J Ethnopharmacol. 2006;104:407-409. https://doi.org/10.1016/j.jep.2005.08.074
  29. Ratnaike RN. Acute and chronic arsenic toxicity. Postgrad Med J. 2003;79:391-396. https://doi.org/10.1136/pmj.79.933.391
  30. Rossman TG. Mechanism of arsenic carcinogenesis: an integrated approach. Mutation Res. 2003;533:37-65. https://doi.org/10.1016/j.mrfmmm.2003.07.009
  31. Shah NC, Joshi MC. An ethnobotanical study of the Kumaon region of India. Economic Botany. 1971;25:414-422. https://doi.org/10.1007/BF02985209
  32. Sharma RK, Rajani GP, Sharma V, Komala N. Effect of ethanolic and aqueous extracts of Bauhinia variegata Linn. on gentamicin-induced nephrotoxicity in rats. Ind J Pharm Edu Res. 2011;45:192-198.
  33. Shon MY, Choi SD, Kahng GG, Nan SH, Sung NJ. Antimutagenic, antioxidant and free radical scavenging activity of ethylacetate from white, yellow and red onions. Food Chem Toxicol. 2004;42:659-666. https://doi.org/10.1016/j.fct.2003.12.002
  34. Simeonova PP, Luster MI. Mechanisms of arsenic carcinogenicity: genetic or epigenetic mechanisms? J Envrion Pathol Toxicol Oncol. 2000;19:281-286.
  35. Sinha A.K. Colorimetric assay of catalase. Analytical Biochem.1972;47:389-394. https://doi.org/10.1016/0003-2697(72)90132-7
  36. Sinha D, Roy S, Roy M. Antioxidant potential of tea reduces arsenite induced oxidative stress in Swiss albino mice. Food Chem Toxicol. 2010;48:1032-1039. https://doi.org/10.1016/j.fct.2010.01.016
  37. Szasz G. Reaction rate method for gamma-glutamyl transferase activity in serum. Clin Chem. 1976; 22:2051-2055.
  38. Vahter M. Metabolism of arsenic. In Biological and environmental effects of arsenic. Fowler BA ed. (Amsterdam, Netherlands: Elsevier), pp. 171-197, 1983.
  39. Wang CH, Hsiao CK, Chen CL, Hsu LI, Chiou HY, Chen SY, Hsueh YM, Wu MM, Chen CJ. A review of the epidemiologic literature on the role of environmental arsenic exposure and cardiovascular diseases. Toxicol Appl Pharmacol. 2007;222:315-326. https://doi.org/10.1016/j.taap.2006.12.022
  40. Vahter M, Concha G. Role of metabolism in arsenic toxicity. Pharmacol Toxicol. 2001;89:1-5. https://doi.org/10.1034/j.1600-0773.2001.d01-128.x
  41. Waalkes MP, Liu J, Diwan BA. Transplacental arsenic carcinogenesis in mice. Toxicol Appl Pharmacol. 2007;222:271-280. https://doi.org/10.1016/j.taap.2006.12.034
  42. Walter K, Schutt C. Acid and alkaline phosphatases in serum (Two point method). Methods in Enzymatic Analysis. Vol 2. Berhmeyer HU ed. (Academic Press, New York), pp. 856-860, 1974.
  43. Yadava RN, Reddy VM. Anti-inflammatory activity of a novel flavonol glycoside from the Bauhinia variegata Linn. Nat Prod Res. 2003;17:165-169. https://doi.org/10.1080/1478641031000104127
  44. Yadava RN, Reddy VMS. A new flavone glycoside 5-hydroxyl 7,3',4',5'-tetramethoxy flavone 5-O-${\beta}$-D-xylopyronosyl ($1{\rightarrow}2$) ${\alpha}$-L-rhamnopyroanoside from Bauhinia variegata Linn. J Asian Nat Prod Res. 2001;3:341-346. https://doi.org/10.1080/10286020108040374