Effects of Bangkeehwangkee-tang ($f{\acute{a}}ngj{\check{i}}hu{\acute{a}}ngq{\acute{i}}-t{\bar{a}}ng$) Treatment on the Monosodium Iodoacetate-induced Osteoarthritis in Rats

방기황기탕이 Monosodium Iodoacetate로 유발된 흰쥐의 골관절염에 미치는 영향

  • Kim, Dae-Hun (Department of Oriental Rehabilitation, College of Oriental Medicine, Dongshin University) ;
  • Kang, Sung-In (Dongshin Hospital) ;
  • Song, Min-Yeong (Department of Oriental Rehabilitation, College of Oriental Medicine, Dongshin University) ;
  • Choi, Jin-Bong (Department of Oriental Rehabilitation, College of Oriental Medicine, Dongshin University) ;
  • Bae, Kil-Joon (Department of Oriental Rehabilitation, College of Oriental Medicine, Dongshin University) ;
  • Han, Jeong-Seok (Department of Oriental Rehabilitation, College of Oriental Medicine, Dongshin University) ;
  • Yang, Mi-Sung (Department of Oriental Rehabilitation, College of Oriental Medicine, Dongshin University) ;
  • Sin, Yong-Jeen (Department of Oriental Rehabilitation, College of Oriental Medicine, Wonkwang University)
  • 김대훈 (동신대학교 한의과대학 한방재활의학교실) ;
  • 강성인 (동신의원) ;
  • 송민영 (동신대학교 한의과대학 한방재활의학교실) ;
  • 최진봉 (동신대학교 한의과대학 한방재활의학교실) ;
  • 배길준 (동신대학교 한의과대학 한방재활의학교실) ;
  • 한정석 (동신대학교 한의과대학 한방재활의학교실) ;
  • 양미성 (동신대학교 한의과대학 한방재활의학교실) ;
  • 신용진 (원광대학교 한의과대학 내과학교실)
  • Received : 2014.03.18
  • Accepted : 2014.04.09
  • Published : 2014.04.30

Abstract

Objectives We have studied to know effects of Bangkeehwangkee-tang on the monosodium iodoacetate (MIA) induced osteoarthritis in rats. Methods Osteoarthritis was induced by injection of monosodium iodoacetate into knee joint cavity of rats. Rats are devided into 4 groups; Sham, control, Bangkeehwangkee-tang, Shinbaro. Sham group was injected by normal saline into knee joint cavity only. Control group was taken no treatment and Experimental groups were taken extracts of Bangkeehwangkee-tang and Shinbaro by orally once a day for 4 weeks. The content of TNF-$\alpha$, IL- $1{\beta}$ and IL-6 in synovial fluids were analysed. COX-2 in chondrocytes and blood PGE2 concentration were analysed. Histopathological examination was performed by Safranin-O fast green staining and Mankins score checking. Results The content of TNF-$\alpha$, IL-$1{\beta}$ in experimental groups were decreased compared with control group. COX-2 in chondrocytes and blood PGE2 concentration in experimental groups were decreased compared with control group. Histopathologically, osteoarthritic scores of experimental groups were decreased compared with control group. But the content of IL-6 in synovial fluids was not significantly compared with control group. Conclusions According to these results, it can be suggested that Bangkeehwangkee-tang has anti-arthritic effects on the MIA induced osteoarthritis in rats.

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