Protective Effect of Clematidis Radix Extract on $CoCl_2$-induced Apoptosis in Human Neuroblastoma Cells

위령선 추출물이 Human Neuroblastoma 세포주에서 $CoCl_2$에 의해 유도된 세포사멸에 미치는 보호효과

  • Park, Jung-Woo (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Gachon University) ;
  • Lim, Hyung-Ho (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Gachon University)
  • 박정우 (가천대학교 한의과대학 한방재활의학과교실) ;
  • 임형호 (가천대학교 한의과대학 한방재활의학과교실)
  • Received : 2014.03.18
  • Accepted : 2014.03.28
  • Published : 2014.04.30

Abstract

Objectives The purpose of this study was to evaluate the effects of Clematidis radix extract on $CoCl_2$-induced apoptosis in SH-SY5Y human neuroblastoma cells. Methods In order to investigate the protective effect of Clematidis radix on $CoCl_2$-induced cytotoxicity in neuronal cells, MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, DAPI(4,6-diamidino-2-phenylindoleI) staining, TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling) assay, DNA fragmentation assay and western blotting were performed on SH-SY5Y human neuroblastoma cells. Results Cells treated with $CoCl_2$ exhibited several apoptotic features, while cells pre-treated with Clematidis radix prior to $CoCl_2$ exposure showed a decrease in the occurrence of apoptotic features. $CoCl_2$ increased HIF-$1{\alpha}$ expression, in contrast, Clematidis radix treatment decreased $CoCl_2$-induced HIF-$1{\alpha}$ expression. Pre-treatment with the extract of Clematidis radix suppressed Bax, cytochrome c, and caspase-3 expressions, and also increased Bcl-2 expression in SH-SY5Y human neuroblastoma cells. Conclusions These results suggest that Clematidis radix may exert a protective effect on $CoCl_2$-induced apoptosis in SH-SY5Y human neuroblastoma cells.

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