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Comparison of Agrobacterium-mediated of Five Alfalfa (Medicago sativa L.) Cultivars Using the GUS Reporter Gene

  • Received : 2014.08.21
  • Accepted : 2014.09.15
  • Published : 2014.09.30

Abstract

Alfalfa (Medicago sativa L.) is one of the most important forage legumes in the world. It has been demanded to establish the efficient transformation system in commercial varieties of alfalfa for forage molecular breeding and production of varieties possessing new characteristics. To approach this, genetic transformation techniques have been developed and modified. This work was performed to establish conditions for effective transformation of commercial alfalfa cultivars, Xinjiang Daye, ABT405, Vernal, Wintergreen and Alfagraze. GUS gene was used as a transgene and cotyledon and hypocotyl as a source of explants. Transformation efficiencies differed from 0 to 7.9% among alfalfa cultivars. Highest transformation efficiencies were observed in the cultivar Xinjiang Daye. The integration and expression of the transgenes in the transformed alfalfa plants was confirmed by polymerase chain reaction (PCR) and histochemical GUS assay. These data demonstrate highly efficient Agrobacterium transformation of diverse alfalfa cultivars Xinjiang Daye, which enables routine production of transgenic alfalfa plants.

Keywords

Alfalfa;Agrobacterium;Molecular breeding;Transgenic plants

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Acknowledgement

Grant : Cooperative Research Program for Agriculture Science & Technology Development

Supported by : National Institute of Animal Science