Effects of Supplementary Cu-Soy Proteinate (Cu-SP) and Herbal Mixture (HBM) on the Growth Performance, Intestinal Microflroa, Immune Response in Broilers

Cu-Soy Proteinate(Cu-SP)와 Herbal Mixture(HBM)의 급여가 육계의 생산성, 소장 내 미생물 균총 및 면역체계에 미치는 영향

  • Kim, Chan Ho (Poultry Science Division, Livestock Resource Development, National Institute of Animal Science, RDA) ;
  • Shin, Kwang Suk (Department of Animal Science and Technology, Chung-Ang University) ;
  • Kang, Hwan Ku (Poultry Science Division, Livestock Resource Development, National Institute of Animal Science, RDA) ;
  • Kim, Ji Hyuk (Poultry Science Division, Livestock Resource Development, National Institute of Animal Science, RDA) ;
  • Hwangbo, Jong (Poultry Science Division, Livestock Resource Development, National Institute of Animal Science, RDA) ;
  • Choi, Hee Cheol (Poultry Science Division, Livestock Resource Development, National Institute of Animal Science, RDA) ;
  • Moon, Hong Kil (Poultry Science Division, Livestock Resource Development, National Institute of Animal Science, RDA) ;
  • Paik, In Kee (Department of Animal Science and Technology, Chung-Ang University) ;
  • Bang, Han Tae (Poultry Science Division, Livestock Resource Development, National Institute of Animal Science, RDA)
  • 김찬호 (국립축산과학원 가금과) ;
  • 신광석 (중앙대학교 동물생명공학과) ;
  • 강환구 (국립축산과학원 가금과) ;
  • 김지혁 (국립축산과학원 가금과) ;
  • 황보종 (국립축산과학원 가금과) ;
  • 최희철 (국립축산과학원 가금과) ;
  • 문홍길 (국립축산과학원 가금과) ;
  • 백인기 (중앙대학교 동물생명공학과) ;
  • 방한태 (국립축산과학원 가금과)
  • Received : 2014.06.10
  • Accepted : 2014.09.02
  • Published : 2014.09.30


The objective of this experiment was to investigate the effect of dietary supplementation of copper-soy proteinate (Cu-SP) and herbal mixture (HBM) on growth performance, intestinal microflora, and immune response in broiler. A total 1,000 1-d old ROSS 308 (initial $BW=41{\pm}0.38g$) were randomly allotted to 1 of 5 dietary treatments with 4 replicates during d 35 of the feeding trial. Dietary included: (1) Control : control diet, (2) Antibiotics : control diet + Avilamycin 6 ppm, (3) Cu-SP : control diet + 100 ppm Cu-soy proteinate, (4) HBM : control diet + 0.15% herbal mixture, (5) Cu-SP+HBM : control diet + 100 ppm Cu-soy proteinate + 0.2% herbal mixture. Two-phase feeding program with a starter diet from d 0 to 21, and a finisher diet from d 22 to 35 was used in the experiment. Within each phase, a diet was formulated to meet or exceed NRC requirements of broilers for macro- and micronutrients. The diet and water were available ad libitum. Result indicated that during d 22 to 35 and over all periods of the experiment, feed intake and feed conversion ratio were greater (P<0.05) for other treatment than control. Significant differences were found in lymphocyte (LY), and stress indicator (HE:LY ratio). Lymphocyte was greater (P<0.05) for Cu-SP + HBM treatment than control. However, stress indicator (HE:LY ratio) were greater (P<0.05) for control than Cu-SP + HBM treatment. The plasma IgG was higher (P<0.05) in the antibiotics, HBM, and Cu-SP+HBM treatments groups compared with control. The population of Clostridium perfringens in the antibiotics, Cu-SP, HBM, Cu-SP + HBM treatment groups were lower (P<0.05) than those control. These result suggested that dietary copper-soy proteinate or herbal mixture may be used as an alternative to antibiotics to improve growth performance, and intestinal health of birds.


Supported by : 국립축산과학원


  1. Baker DH, Odle J, Funk MA, Wieland TM 1991 Bioavailability of copper in cupric oxide, cuprous, and in a copperlysine complex. Poult Sci 70:171-179.
  2. Burnell TW, Cromwell GL, Stahly TS 1988 Cited by J Gohl in bottom line of nutrition. Feedstuff. June 13. pp 16-18.
  3. Cromwell GL, Stahly TS, Mongue HJ 1989 Effect of source and level of copper on performance and liver copper stores in weanling pig. J Anim Sci 67:2996-3002.
  4. Cross DE, Acamovic T, Deans SG, Cdevitt RM 2007 The effects of dietary inclusion of herbs and their volatile oils on performance, dietary digestibility and gut microflora in chickens from 7 to 28 days of age. Br. Poult Sci 48:496-506.
  5. Fisher C, Luren-Jonse AP, Hill KJ, Hardy WS 1973 The effect of copper sulfate on performance and the structure of gizzard in broilers. Br Poult Sci 14:55-68.
  6. Kamel C 2001 Tracing modes of action and the roles plant extracts in non-ruminants. In: Recent Advances in Animal Nutrition (Gaemsworthy PC, Wisenman J). Nottingham University Press. pp. 135-150.
  7. Kim G-B, Seo YM, Shin KS, Rhee AR, Han J, Paik IK 2011. Effects of supplemental copper-methionine chelate and copper-soy proteinate on the performance, blood parameters, liver mineral content, and intestinal microflora of broiler chickens. J Appl Poult Res 20:21-32.
  8. Klasing CK 1988 Minerals. Comparative Avian Nutrition. CAB International. New York. USA. pp 234-276.
  9. Mancini G, Carnonara AO, Heremans JF 1965. Immuochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry 2:235-254.
  10. Melvin JS 1984 Physiological properties and cellular and chemical constituents of blood. Duke's Physiological of Domestic Animals. 10th Ed. Cornell University Press.
  11. NRC 1994 Nutrient Requirements of Poultry. National Research Council. National Academy of Science. Washington, DC.
  12. Paik IK, Seo SH, Um JS, Chang MB, Lee BH 1999 Effect of supplementary copper-chelate on the performance and cholesterol level in plasma and breast muscle of broiler chickens. Asian-Aust J Anim Sci 12:794-798.
  13. SAS 2012 SAS/STAT Software for PC. SAS Institute. Cary, NC, USA.
  14. Williams P and Losa R 2001 The use of essential oils and their compounds in poultry nutrition. Worlds Poult Sci 17:14-15.
  15. 곽이성 양재원 이광승 1993 일부 병원성 미생물에 대해 항균활성을 보이는 생약의 탐색. 식품위생학회지 8:141-145.
  16. 권오진 육홍선 차보숙 변명우 1996 한약재 추출물의 항균활성에 대한 감마선 조사의 영향. 식품위생안전학회지 11:209-214.
  17. 김윤기 백인기 1993 구리 공급원과 첨가수준이 육계의 생산성에 미치는 영향. 한국축산학회지 35:52-59.
  18. 김찬호 백인기 2008 허브 및 식물 추출물의 급여가 산란계의 생산성에 미치는 영향. 한국가금학회지 35:71-78.
  19. 민승기 남궁환 백인기 1993 사료첨가제로서 구리복합제가 육계의 생산성에 미치는영향. 한국영양사료학회지 17:247-257.
  20. 민승기 엄재상 백인기 1994 메티오닌-구리 및 단백질-구리 복합제가 육계와 쥐의 성장과 광물질 대사 및 장내 균총에 미치는 영향. 한국영양사료학회지 18:103-113.
  21. 백인기 김찬호 박광월 2008 사료 내 Cu-methionine chelaate와 Cu-soy proteinate가 산란계의 생산성, 소장 내 미생물 균총 및 면역체계에 미치는 영향. 한국가금학회지 35:303-311.
  22. 손경승 권오석 민병준 조진호 진영걸 김인호 김홍수 2004 허브제품 (Animuniun Powder)의 급여가 산란계의 계란 품질과 혈액성상 및 영양소 소화율에 미치는 영향. 한국가금학회지 31:237-244.
  23. 신현경 1995 산채류가 장내세균의 생육에 미치는 영향 연구. 한국음식문화연구원 논문집 6:336-354.
  24. 오덕환 함승시 박부길 안철 유진영 1998 식품부패 및 병원성 미생물에 대한 천연 약용식물 추출물의 항균효과. 한국식품과학회지 30:957-963.
  25. 우경천 김찬호 남궁년 백인기 2007 생약제 (Herbs, Plant extracts)의 급여가 육계의 생산성에 미치는 영향. 한국가금학회지 34:43-52.
  26. 이문구 김찬호 신동훈 정병윤 백인기 2011 구리 공급원들 (Cu Sulfate, Cu-methionine, Cu-soy proteinate)의 첨가가 육계의 생산성에 미치는 영향. 한국가금학회지 38:121-128.
  27. 이우선 백인기 2007a 개량한방제제(Herb Mix Gold) 첨가가 산란계 생산성에 미치는 영향. 한국가금학회지 34:37-42.
  28. 이우선 백인기 2007b 닭의 성장과 산란 생산성 개선 효과 증대를 위한 한방제제 (Herb Mix)의 개량에 관한 연구. 한국가금학회지 34:245-251.
  29. 이우선 백인기 2007c Herb Mix 첨가가 이유자돈 생산성에 미치는 영향. 한국동물자원과학회지 49:321-328.
  30. 이인선 하영득 1994 생약제가 면역세포 활성화에 미치는 영향. 한국영양식량확회지 49:321-328.
  31. 이한규 2005 Fe-soy proteinate를 이용한 철분강화계육 및 계란생산에 관한 연구. 제103회 중앙대학교 석사학위논문.
  32. 홍성진 남궁환 백인기 2002 생약제제 (Mircle20)가 육계의 생산성과 영양소 이용율, 소장 내 미생물 균총 및 면역기능에 미치는 영향. 한국동물자원과학회지 43:671-680.

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