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Analytical Methods of Fenpyroximate in Herbal Medicines

생약 중 Fenpyroximate의 분석법 연구

  • Lee, Ju-Hee (Hazardous Substances Analysis Division, Daegu Regional Food & Drug Administration) ;
  • Lee, Yoon-Jeong (Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation) ;
  • Kang, In-Ho (Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation) ;
  • Kim, Do-Hoon (Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation) ;
  • Kang, Shin-Jeong (Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation) ;
  • An, Kyung-A (Hazardous Substances Analysis Division, Daegu Regional Food & Drug Administration) ;
  • Lee, Ryun-Kyung (Hazardous Substances Analysis Division, Daegu Regional Food & Drug Administration) ;
  • Suh, Sang-Chul (Hazardous Substances Analysis Division, Daegu Regional Food & Drug Administration) ;
  • Lee, Jeong-Rim (Hazardous Substances Analysis Division, Daegu Regional Food & Drug Administration)
  • 이주희 (대구지방식품의약품안전청 유해물질분석과) ;
  • 이윤정 (식품의약품안전평가원 생약연구과) ;
  • 강인호 (식품의약품안전평가원 생약연구과) ;
  • 김도훈 (식품의약품안전평가원 생약연구과) ;
  • 강신정 (식품의약품안전평가원 생약연구과) ;
  • 안경아 (대구지방식품의약품안전청 유해물질분석과) ;
  • 이륜경 (대구지방식품의약품안전청 유해물질분석과) ;
  • 서상철 (대구지방식품의약품안전청 유해물질분석과) ;
  • 이정림 (대구지방식품의약품안전청 유해물질분석과)
  • Received : 2014.05.16
  • Accepted : 2014.07.10
  • Published : 2014.09.30

Abstract

Fenpyroximate is acaricide of pyrazole group. This acaricide have already been permitted for herb cultivation. This experiment was conducted to establish a determination method for fenpyroximate residue in herbal medicines using HPLC-PDA and HPLC-MS/MS. Fenpyroximate residue was extracted with acetone from samples of herbal which Liquorice Root (Glycyrrhiza uralensis) and Safflower Seed (Carthamus tinctorius Linne). The extract was diluted with saturated saline water and dichloromethane liquid-liquid partition (extraction) was followed to recover fenpyroximate from the aqueous phase. Amino propyl ($NH_2$) and florisil column chromatography was additionally employed for final clean up of the extract. The fenpyroximate was quantitated by HPLC-PDA and HPLC-MS/MS. The herbals were fortified with fenpyroximate at 2 or 3 levels per crop. Mean recovery ratio were ranged from 72.0 to 106.4%. The coefficients of variation were ranged from 0.2 to 4.4. Therefore, this analytical method was reproducible and sensitive enough to determine the residue of fenpyroximate in herbal medicines.

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