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Monitoring for the Resistance of Strobilurin Fungicide Against Botrytis cinerea Causing Gray Mold Disease

Strobilurin계 살균제에 대한 잿빛곰팡이병균 Botrytis cinerea의 저항성 검정

  • Kim, Ah Hyeong (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University) ;
  • Kim, Seon Bo (Sungbo Chemical) ;
  • Han, Ki Don (Sungbo Chemical) ;
  • Kim, Heung Tae (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University)
  • 김아형 (충북대학교 농업생명환경대학 식물의학과) ;
  • 김선보 (성보화학(주)) ;
  • 한기돈 (성보화학(주)) ;
  • 김흥태 (충북대학교 농업생명환경대학 식물의학과)
  • Received : 2014.05.29
  • Accepted : 2014.06.30
  • Published : 2014.09.30

Abstract

This study was conducted to investigate the resistance of Botrytis cinerea to azoxystrobin, which belongs to strobilurin fungicides. The sensitivity of B. cinerea isolates, which were collected from infected pepper, strawberry, cucumber and tomato by a single spore isolation, to the fungicide was tested through a agar dilution method on PDA amended with fungicides and $100{\mu}g\;mL^{-1}$ of salicylhydroxamic acid (SHAM). All isolates of B. cinerea tested in this study were classified as a sensitive and a resistant group by $1.0{\mu}g\;mL^{-1}$ of $EC_{50}$ value to azoxystrobin. While the sensitive isolates accounted for 46.5% of B. cinerea population, the resistant ones did for 53.5%. According to the regions isolating B. cinerea, the highest isolation frequency was showed as 81.1% in Chungnam among the all. Among 4 host plants as pepper, strawberry, cucumber and tomato, the highest isolation frequency was obtained in strawberry, while the lowest was done in pepper. The isolate resistant to azoxystrobin showed the cross resistance to other fungicides included into strobilurins as kresoxim-methyl and trifloxystrobin. In spite of an excellent efficacy of strobilurins, it should be taken care to use them in the field, because of the high risk in the fields.

Acknowledgement

Supported by : 충북대학교

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