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Anti-inflammatory Activities of Fermented Black Garlic

흑마늘 발효물의 항염증 활성

  • Tak, Hyun-Min (Namhae Garilic Research Institute) ;
  • Kang, Min-Jung (Namhae Garilic Research Institute) ;
  • Kim, Kyoung Min (Namhae Garilic Research Institute) ;
  • Kang, Dawon (Department of Physiology and Institute of Health Sciences, Gyeongsang National University School of Medicine) ;
  • Han, Sunkyu (Department of Physiology and Institute of Health Sciences, Gyeongsang National University School of Medicine) ;
  • Shin, Jung-Hye (Namhae Garilic Research Institute)
  • 탁현민 ((재)남해마늘연구소) ;
  • 강민정 ((재)남해마늘연구소) ;
  • 김경민 ((재)남해마늘연구소) ;
  • 강다원 (경상대학교 의학전문대학원 생리학교실) ;
  • 한선규 (경상대학교 의학전문대학원 생리학교실) ;
  • 신정혜 ((재)남해마늘연구소)
  • Received : 2014.06.13
  • Accepted : 2014.08.01
  • Published : 2014.10.31

Abstract

In this study, we investigated the anti-inflammatory effects of Lactobacillus rhamnosus fermented black garlic (FBG) in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. FBG did not show cytotoxicity in RAW 264.7 cells at concentrations less than $800{\mu}g/mL$, and cell viability increased with FBG concentration. Nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) production as well as tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), interleukin-$1{\beta}$ (IL-$1{\beta}$) and IL-6 formation decreased in an FBG concentration-dependent manner, in LPS-induced RAW 264.7 cells. Furthermore, activation of LPS-inducible nitric synthase (iNOS), cyclooxygenase-2 (COX-2), nuclear factor kappa B (NF-${\kappa}B$), and inhibitory kappa B ($I{\kappa}B$) protein expression was effectively inhibited by FBG treatment in LPS-induced RAW 264.7 cells. In contrast, heme oxygenase-1 (HO-1) protein expression significantly increased. These results indicate that the anti-inflammatory activity of FBG was due to activation of NF-${\kappa}B$, inhibition of cytokine production, and expression of iNOS and COX-2. From these results, we expect that FBG could contribute to the prevention and improvement of inflammatory disease.

Keywords

fermented black garlic (FBG);cytokine;anti-inflammatory;RAW 264.7 cells

Acknowledgement

Supported by : 산업통상자원부

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