Effects of Yoohyangheukho-dan ($R{\check{u}}xi{\bar{a}}ngh{\bar{e}}ih{\check{u}}-d{\bar{a}}n$) on Osteoporosis Induced by Ovariectomy in Rats

유향흑호단(乳香黑虎丹)이 난소적출로 유발된 흰쥐의 골다공증 모델에 미치는 영향

  • Received : 2014.06.16
  • Accepted : 2014.07.15
  • Published : 2014.07.31


Objectives Osteoporosis is an impending problem in the rapidly aging society. The purpose of this study is to identify the effects of Yoohyangheukho-dan ($R{\check{u}}xi{\bar{a}}ngh{\bar{e}}ih{\check{u}}-d{\bar{a}}n$) on osteoporosis induced by ovariectomy in rats. Methods 24 rats were randomly assigned to a SHAM group, a control group, and a Yoohyangheukho-dan ($R{\check{u}}xi{\bar{a}}ngh{\bar{e}}ih{\check{u}}-d{\bar{a}}n$) group (n=8). Ovaries were extracted and for 8 weeks, the rats were given dry feeds and Yoohyangheukho-dan ($R{\check{u}}xi{\bar{a}}ngh{\bar{e}}ih{\check{u}}-d{\bar{a}}n$) group were given a mixture of dry feeds and Yoohyangheukho-dan ($R{\check{u}}xi{\bar{a}}ngh{\bar{e}}ih{\check{u}}-d{\bar{a}}n$). At 8 weeks, their body weight, femur mass, tibia ash per body ratio, overall surface area and thickness of the trabeculae, overall surface area of the osteoblasts, and the number of osteoclasts were measured and levels of albumin, aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), total-cholesterol, calcium and estradiol were recorded. Results There was no significant difference in the weight of the femur and the tibia ash per body ratio. The Yoohyangheukho-dan ($R{\check{u}}xi{\bar{a}}ngh{\bar{e}}ih{\check{u}}-d{\bar{a}}n$) group had significantly thicker trabeculae than the control group and showed a minimal increase of overall surface area of the trabeculae. The overall surface area of the osteoblasts and the number of osteoclasts decreased in the Yoohyangheukho-dan ($R{\check{u}}xi{\bar{a}}ngh{\bar{e}}ih{\check{u}}-d{\bar{a}}n$) group. There were no statistically significant differences in AST, ALT, ALP, total cholesterol, phosphorus, and estradiol levels. On the contrary the Yoohyangheukho-dan ($R{\check{u}}xi{\bar{a}}ngh{\bar{e}}ih{\check{u}}-d{\bar{a}}n$) group had significantly higher levels of Albumin, triglyceride, and calcium. Conclusions It can be concluded that oral intake of Yoohyangheukho-dan ($R{\check{u}}xi{\bar{a}}ngh{\bar{e}}ih{\check{u}}-d{\bar{a}}n$) prevents the thinning process of the trabeculae. Thus, it may have positive effects on the treatment and prevention of osteoporosis in postmenopausal women.


Yoohyangheukho-dan ($R{\check{u}}xi{\bar{a}}ngh{\bar{e}}ih{\check{u}}-d{\bar{a}}n$);Osteoporosis;Postmenopausal osteoporosis;Osteoblast;Osteoclast


  1. 건강보험심사평가원 심사평가연구소 통계정보센터. 건강보험심사평가원 보건의료동향 보도자료. 2013:1-4.
  2. Anonymous. Consensus development conference:diagnosis, prophylaxis, and treatment of osteoporosis. The American Journal of Medicine. 1993;94:646-50. https://doi.org/10.1016/0002-9343(93)90218-E
  3. Elizabeth A, Chrischilles C, David Butler, Charles S Davis, Robert B, Wallace. A model of lifetime osteoporosis impact. Arch Intern Med. 1991;151(10):2026-32. https://doi.org/10.1001/archinte.1991.00400100100017
  4. Cooper C, Campion G, Melton LJ 3rd. Hip fractures in the elderly: a world-wide projection. Osteoporos Int. 1992;2:285-9. https://doi.org/10.1007/BF01623184
  5. 박예수. 골다공증의 진단과 치료. J Korean Med Assoc. 2012;55(11):1083-94. https://doi.org/10.5124/jkma.2012.55.11.1083
  6. Rozenberg S, Kroll M, Pastijn A, Vandromme J. Osteoporosis prevention and treatment with sex hormone replacement therapy. Clinical Rheumatology. 1995;14(3): 14-7.
  7. 최진경, 유동열. 加味左歸飮이 난소적출로 유발된 백서의 골다공증에 미치는영향. 대한한방부인과학회지. 2006;19(2): 107-26.
  8. Graham A Colditz, Susan E Hankinson, David J Hunter, Walter C Willett, JoAnn E Manson, Meir J Stampfer, Charles Hennekens, Bernard Rosner, Frank E Speizer. The use of estrogens and progestins and the risk of breast cancer in postmenopausal women. New England Journal Clinical Rheumatology. 1995;332:1589-93.
  9. 全國韓醫科大學原典學敎室. 類編黃帝內經. 서울:주민출판사. 2006:918-920, 943-944, 953-4.
  10. 韓方再活醫學科學會. 韓方再活醫學. 서울:군자출판사. 2012: 9-10, 165-169.
  11. 이동녕. A Study of Yukmijihwangtang-Jahage extract on Regulation of Bone Resorption. 한방부인과학회지. 2003;16(1):62-80.
  12. 정재숙, 서일복, 김형준. 生血補髓湯 이 난소적출 흰쥐 대퇴골의 형태계측학적 변화 및 골대사 관련인자에 미치는 영향. 대한한방부인과학회지. 2005;18(3):32-50.
  13. 김덕님, 공복철, 김양호, 김송백, 조한백. 滋陰八味煎이 난소적출백서의 골다공증에 미치는 영향. 대한한방부인과학회지. 2005;18(2):12-25.
  14. 곽남길. 人蔘養榮湯이 난소적출로 유발된 흰쥐의 골다공증 모델에 미치는 영향. 경희대학교대학원. 2013:25-32.
  15. 許浚. 東醫寶鑑. 서울:민중서원. 1993:649-53.
  16. Saville PD. Changes in skeletal mass and fragility with castration in the rat:a model of osteoporosis. Journal of the American Geriatrics Society. 1969;17:155-66. https://doi.org/10.1111/j.1532-5415.1969.tb03169.x
  17. Wronski TJ, Lowry PL, Walsh CC, Ignaszewski LA. Skeletal Alterations in Ovariectomized Rats. Calcif Tissue lnt. 1985;37:324-8. https://doi.org/10.1007/BF02554882
  18. Wronski TJ, Schenck PA, Cintr6n M, Walsh CC. Effect of Body Weight on Osteopenia in Ovariectomized Rats. Calcif Tissue lnt. 1987;40:155-9. https://doi.org/10.1007/BF02555700
  19. Yamzaki I, Yamaguchi H. Characteristics of an ovariectomized rat model. J Bone miner Res. 1989;4(1):13-22.
  20. Hughes DE, Dai A, Tiffee JC, Li HH, Mundy GR, Boyce BF. Estrogen promotes apoptosis of murine osteoclasts mediated by TGF-beta. Nature Medicine. 1996;2:1132-6. https://doi.org/10.1038/nm1096-1132
  21. 이귀녕, 이종순. 임상병리파일. 서울:의학문화사. 1996:171-2, 278-82, 229-32, 300-3, 305-9.