Anti-allergic Effects of Samhwangsasim-tang ($S{\bar{a}}nhu{\acute{a}}ngxi{\grave{e}}x{\bar{i}}nt{\bar{a}}ng$) on Ovalbumin-induced Allergic Model in Mice

삼황사심탕(三黃瀉心湯)이 난황 알부민으로 유도된 알레르기 Mouse모델에서 항알레르기 효과

  • Choi, Chong-Hwan ;
  • Keum, Seon-Oh ;
  • Lee, Se-Won ;
  • Kim, Il-Hyun ;
  • Lee, Ha-Il ;
  • Song, Yung-Sun
  • 최종환 ;
  • 금선오 ;
  • 이세원 ;
  • 김일현 ;
  • 이하일 ;
  • 송용선
  • Received : 2014.06.18
  • Accepted : 2014.07.05
  • Published : 2014.07.31

Abstract

Objectives In this study, we investigated the inhibitory effects of Samhwangsasim-tang (S.H) on the allergic response caused by ovalbumin(OVA) sensitization and challenge in BALB/c mice. Methods The experimental animals were divided into five groups; 1) normal as negative control, 2) OVA-sensitized mice, 3) OVA-sensitized mice treated with 200 mg/kg of S.H 200, 4) OVA-sensitized mice treated with 400 mg/kg of S.H 400, and 5) OVA-sensitized mice treated with 5 mg/kg of Dexamethasone (Dex). Antigen sensitization for allergic mouse model was performed with twice injection of OVA for 2 weeks. After secondary injection, S.H was administrated orally into mice every day for 13 days and the inhibitory effect of S.H on allergic responses was evaluated. Results Treatment of S.H into allergic mice reduced significantly ear edema and infiltration of immune cells in ear tissues induced with OVA challenge in a dose-dependent manner. S.H reduced significantly the serum levels of Total Immunoglobulin(Ig)G and IgE, and particularly inhibited the production of OVA-specific IgE, but not OVA-specific IgG. The serum level of proinflammatory cytokine TNF-${\alpha}$ and Th2-associated cytokine IL-4 also were significantly decreased by S.H adminstration in a dose denpendent manner. S.H attenuated OVA-induced secretion of IFN-${\gamma}$, but not IL-12 which is a cytokine inducing the development of Th1 cells. It also reduced significantly the secretion of IL-4, which is a cytokine inducing the development of Th2 cells, after splenocytes were stimulated with OVA. However the secretion of IL-5 and IL-13 was influenced weakly or a little. Conclusions These results indicate that S.H could reduce the allergic response through inhibition of antigen-specific IgE and Th2-inducing cytokines. It suggest that S.H may be available clinically for the treatment of allergic patients.

Keywords

Samhwangsasim-tang (S.H);Allergic disease;Ovalbumin;Immunoglobulin;proinflammatory cytokine;Interleukin-4

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Acknowledgement

Supported by : 원광대학교