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Evaluation of Dietary Multiple Enzyme Preparation (Natuzyme) in Laying Hens

  • Lee, K.W. (Department of Animal Science and Technology, Konkuk University) ;
  • Choi, Y.I. (Department of Animal Science and Technology, Konkuk University) ;
  • Moon, E.J. (Department of Animal Science and Technology, Konkuk University) ;
  • Oh, S.T. (Department of Animal Science and Technology, Konkuk University) ;
  • Lee, H.H. (Department of Animal Science and Technology, Konkuk University) ;
  • Kang, C.W. (Department of Animal Science and Technology, Konkuk University) ;
  • An, B.K. (Department of Animal Science and Technology, Konkuk University)
  • Received : 2014.04.19
  • Accepted : 2014.06.26
  • Published : 2014.12.01

Abstract

The current experiment was designed to evaluate the efficacy of adding the multi-enzyme mixture (Natuzyme) into layers' diets with different levels of energy and available phosphorus in relation to laying performance, egg qualities, blood cholesterol level, microflora and intestinal viscosity. Two hundred and fifty 43-wk-old Hy-Line commercial layers were divided into five groups with five replicates per group (10 birds per replicate) and fed one of five experimental diets. A corn and soybean meal-based control diet was formulated and used as a control diet. Two experimental control diets were formulated to reduce energy and crude protein contents (rE) or energy, crude protein and phosphorus contents (rEP). In addition, Natuzyme was added into either rE (rE-Natu500) or rEP (rEP-Natu500) diet to reach a concentration of 500 mg per kg of diet. The experiment lasted 8 weeks. There were no significant differences in feed intake, egg production, egg weight, egg qualities such as eggshell color or Haugh unit, total cholesterol, relative organ weights and cecal microflora profiles between any dietary treatments. Natu500 supplementation into the rE diet, but not rEP diet significantly increased egg mass and eggshell qualities such as strength and thickness, but it decreased cecal ammonia concentration and intestinal viscosity in laying hens. In conclusion, the present study shows that adding multiple enzyme preparation could improve performance of laying hens fed energy and protein restricted diets.

Keywords

Multiple Enzyme Preparation;Egg Qualities;Performance;Intestinal Viscosity

Acknowledgement

Supported by : KonKuk University

References

  1. Adeola, O. and A. J. Cowieson. 2011. Board-invited review: Opportunities and challenges in using exogenous enzymes to improve nonruminant animal production. J. Anim. Sci. 89:3189-3218. https://doi.org/10.2527/jas.2010-3715
  2. Annison, G. and M. Choct. 1991. Anti-nutritive activities of cereal non-starch polysaccharides in broiler diets and strategies minimizing their effects. World's Poult. Sci. J. 47:232-242. https://doi.org/10.1079/WPS19910019
  3. Han, S. W., K. W. Lee, B. D. Lee, and C. G. Sung. 1999. Effect of feeding Aspergillus oryzae culture on fecal microflora, egg qualities, and nutrient metabolizabilities in laying hens. Asian Australas. J. Anim. Sci. 12:417-421. https://doi.org/10.5713/ajas.1999.417
  4. Bedford, M. R. and H. Schulze. 1998. Exogenous enzymes in pigs and poultry. Nutr. Res. Rev. 11:91-114. https://doi.org/10.1079/NRR19980007
  5. Bedford, M. R. and C. Wyatt. 1998. Using feed enzymes to maximize nutrient utilization in corn-soy or sorghum-soy based layer diets. Zootecnica Int. 9:32-35.
  6. Cowieson, A. J. and O. Adeola. 2005. Carbohydrase, protease, and phytase have an additive beneficial effect in nutritionally marginal diets for broiler chicks. Poult. Sci. 84:1860-1867. https://doi.org/10.1093/ps/84.12.1860
  7. Jang, H. D., Y. Hyun, H. S. Kim, I. W. Hwang, J. S. Yoo, H. J. Kim, S. O. Shin, Y. Hwang, T. X. Zhou, Y. J. Chen, J. H. Cho, and I. H. Kim. 2008. Effect of dietary microbial phytase on laying performance, egg quality, phosphorus utilization and nutrient metabolizability in laying hens. Korean J. Poult. Sci. 35:115-121. https://doi.org/10.5536/KJPS.2008.35.2.115
  8. Lazaro, R., M. Garcia, P. Medel, and G. G. Mateos. 2003. Influence of enzyme on performance and digestive parameters of broiler fed rye-based diets. Poult. Sci. 82:132-140. https://doi.org/10.1093/ps/82.1.132
  9. Lee, S. Y., J. S. Kim, J. M. Kim, B. K. An, and C. W. Kang. 2010. Effects of multiple enzyme of NSP enzymes and phytase (ROVABIO$^{(R)}$Max) supplemented to corn-wheat-soybean meal based diets on growth performance and intestinal viscosity in broiler chicks. Asian Australas. J. Anim. Sci. 23:1198-1204. https://doi.org/10.5713/ajas.2010.90592
  10. Lee, K.W., H. S. Lillehoj, S. I. Jang, S. H. Lee, D. A. Bautista, and G. R. Siragusa. 2013. Effect of Bacillus subtilis-based direct-fed microbials on immune status in broiler chickens raised on fresh or used litter. Asian Australas. J. Anim. Sci. 26:1592-1597. https://doi.org/10.5713/ajas.2013.13178
  11. Lee, K. W., G. X. Li, H. S. Lillehoj, S. H. Lee, S. I. Jang, U. S. Babu, E. P. Lillehoj, A. P. Neumann, and G. R. Siragusa. 2011. Bacillus subtilis-based direct-fed microbials augment macrophage function in broiler chickens. Res. Vet. Sci. 91:e87-e91. https://doi.org/10.1016/j.rvsc.2011.01.018
  12. Lillehoj, H. S. and K. W. Lee. 2012. Immune modulation of innate immunity as alternatives-to-antibiotics strategies to mitigate the use of drugs in poultry production. Poult. Sci. 91:1286-1291. https://doi.org/10.3382/ps.2012-02374
  13. Lim, H. S., H. Namkung, and I. K. Paik. 2003. Effects of phytase supplementation on the performance, egg quality, and phosphorus excretion of laying hens fed different levels of dietary calcium and nonphytate phosphorus. Poult. Sci. 82:92-99. https://doi.org/10.1093/ps/82.1.92
  14. Liu, J. R., S. F. Lai, and B. Yu. 2007. Evaluation of an intestinal lactobacillus reuteri strain expressing rumen fungal xylanase as a probiotic for broiler chickens fed on a wheat-based diet. Br. Poult. Sci. 48:507-514. https://doi.org/10.1080/00071660701485034
  15. National Research Council. 1994. Nutrient requirements of poultry. 9th ed. National Academy Press. Washington DC, USA.
  16. Ravindran, V. 2013. Feed enzymes: The science, practice, and metabolic realities. J. Appl. Poult. Res. 22:628-636. https://doi.org/10.3382/japr.2013-00739
  17. SAS. 2002. SAS user's guide. Statistics, Version 8. e., SAS Institute. Inc. Cary, NC, USA.

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