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Screening of Differential Promoter Hypermethylated Genes in Primary Oral Squamous Cell Carcinoma

  • Khor, Goot Heah (Centre of Preclinical Science Studies, Faculty of Medicine, Universiti Teknologi MARA) ;
  • Froemming, Gabrielle Ruth Anisah (Institute of Medical Molecular Biotechnology, Faculty of Medicine, Universiti Teknologi MARA) ;
  • Zain, Rosnah Binti (Oral Cancer Research and Coordinating Centre, Faculty of Dentistry, University of Malaya) ;
  • Abraham, Mannil Thomas (Tengku Ampuan Rahimah Hospital, Department of Oral and Maxillofacial Surgery, Ministry of Health Malaysia) ;
  • Thong, Kwai Lin (Laboratory of Biomedical Science and Molecular Microbiology, UMBIO Cluster, Institute of Graduate Studies, University of Malaya)
  • Published : 2014.11.06

Abstract

Background: Promoter hypermethylation leads to altered gene functions and may result in malignant cellular transformation. Thus, identification of biomarkers for hypermethylated genes could be useful for diagnosis, prognosis, and therapeutic treatment of oral squamous cell carcinoma (OSCC). Objectives: To screen hypermethylated genes with a microarray approach and to validate selected hypermethylated genes with the methylation-specific polymerase chain reaction (MSPCR). Materials and Methods: Genome-wide analysis of normal oral mucosa and OSCC tissues was conducted using the Illumina methylation microarray. The specified differential genes were selected and hypermethylation status was further verified with an independent cohort sample of OSCC samples. Candidate genes were screened using microarray assay and run by MSPCR analysis. Results: TP73, PIK3R5, and CELSR3 demonstrated high percentages of differential hypermethylation status. Conclusions: Our microarray screening and MSPCR approaches revealed that the signature candidates of differentially hypermethylated genes may possibly become potential biomarkers which would be useful for diagnostic, prognostic and therapeutic targets of OSCC in the near future.

Keywords

Microarray;oral squamous cell carcinoma;promoter hypermethylation;MSPCR

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