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Drug Release from Thermosensitive Liposomes by High-Intensity Focused Ultrasound

고강도 집속 초음파에 의한 온도민감성 리포솜으로부터 약물 방출

  • Received : 2014.06.25
  • Accepted : 2014.08.29
  • Published : 2014.12.20

Abstract

Development of liposomes has been actively studied for effective delivery of drug at tumor site. However, despite their preferential accumulation at tumor site, the therapeutic efficacy of such liposomal drug has been limited because of low drug release. Therefore, we developed a temperature-sensitive liposome (TSL), which can be made to maximize release of drug by external stimulation such as ultrasound. Doxorubicin (DOX) as a model drug was encapsulated into TSL by a pH gradient method. The particle size of the TSL was $142.0{\pm}6.24nm$. Surface charge was $-10.55{\pm}1.12mV$. Release of drug from TSLs was up to 80% within 15 min at over $42^{\circ}C$ measured by fluorescence intensity. Cytotoxicity of released DOX from TSLs with ultrasound was highly increased compared to TSLs without ultrasound. Taken together, we demonstrate that temperature sensitive drug release from TSLs with ultrasound, which may be useful for cancer therapy to increase drug concentration at tumor site by external stimulation.

Keywords

Liposome;Drug release;Doxorubicin;Ultrasound

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Acknowledgement

Supported by : 교육과학기술부