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Isolation and Characterization of the Colletotrichum acutatum ABC Transporter CaABC1

  • Kim, Suyoung (Department of Biomaterial Control, Dongeui University) ;
  • Park, Sook-Young (Korean Lichen Research Institute, Sunchon National University) ;
  • Kim, Hyejeong (Department of Biomaterial Control, Dongeui University) ;
  • Kim, Dongyoung (Department of Biomaterial Control, Dongeui University) ;
  • Lee, Seon-Woo (Department of Applied Biology, Dong-A University) ;
  • Kim, Heung Tae (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University) ;
  • Lee, Jong-Hwan (Department of Biomaterial Control, Dongeui University) ;
  • Choi, Woobong (Department of Biomaterial Control, Dongeui University)
  • Received : 2014.08.18
  • Accepted : 2014.09.28
  • Published : 2014.12.01

Abstract

Fungi tolerate exposure to various abiotic stresses, including cytotoxic compounds and fungicides, via their ATP-driven efflux pumps belonging to ATP-binding cassette (ABC) transporters. To clarify the molecular basis of interaction between the fungus and various abiotic stresses including fungicides, we constructed a cDNA library from germinated conidia of Colletotrichum acutatum, a major anthracnose pathogen of pepper (Capsicum annum L.). Over 1,000 cDNA clones were sequenced, of which single clone exhibited significant nucleotide sequence homology to ABC transporter genes. We isolated three fosmid clones containing the C. acutatum ABC1 (CaABC1) gene in full-length from genomic DNA library screening. The CaABC1 gene consists of 4,059 bp transcript, predicting a 1,353-aa protein. The gene contains the typical ABC signature and Walker A and B motifs. The 5'-flanking region contains a CAAT motif, a TATA box, and a Kozak region. Phylogenetic and structural analysis suggested that the CaABC1 is a typical ABC transporter gene highly conserved in various fungal species, as well as in Chromista, Metazoans, and Viridiplantae. We also found that CaABC1 was up-regulated during conidiation and a minimal medium condition. Moreover, CaABC1 was induced in iprobenfos, kresoxim-methyl, thiophanate-methyl, and hygromycin B. These results demonstrate that CaABC1 is necessary for conidiation, abiotic stress, and various fungicide resistances. These results will provide the basis for further study on the function of ABC transporter genes in C. acutatum.

Acknowledgement

Supported by : Dongeui University

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