DOI QR코드

DOI QR Code

Genetic Variation in MicroRNAs and Risk of Oral Squamous Cell Carcinoma in South Indian Population

  • Sushma, PS (Department of Pathology, National Institute of Nutrition (ICMR)) ;
  • Jamil, Kaiser (Department of Genetics, Bhagwan Mahavir Medical Research Centre) ;
  • Kumar, P Uday (Department of Pathology, National Institute of Nutrition (ICMR)) ;
  • Satyanarayana, U (Department of Biochemistry, Dr. Pinnamaneni Siddhartha Institute of Medical Sciences) ;
  • Ramakrishna, M (MNJ Institute of Oncology and Regional Cancer Centre) ;
  • Triveni, B (MNJ Institute of Oncology and Regional Cancer Centre)
  • Published : 2015.12.03

Abstract

Background: MicroRNAs (miRNAs) are small non-coding RNA molecules, implicated in several activities like initiation, progression and prognosis of various cancers. Single nucleotide polymorphisms (SNPs) in miRNA genes can lead to alteration in mRNA expression, resulting in diverse functional consequences. The aim of our study was to investigate the association of miR-149C>T and miR-196a2C>T SNPs with susceptibility to development of oral squamous cell carcinoma (OSCC) in South Indian subjects. Materials and Methods: 100 OSCC patients and 102 healthy controls from the general population were recruited for the study. Genetic analysis was performed by polymerase chain reaction/restriction fragment length polymorphism (PCR/RFLP) as per a standard protocol. Results: The genotype frequencies in miR-196a2 polymorphism, of TT, CT and CC in the OSCC patients were 69%,10% and 22% respectively while for control group it was 80%, 15% and 5% respectively. The CC genotype of miR196a2 polymorphism was significantly associated with oral squamous cell carcinoma. The genotype frequencies in miR-149 polymorphisms of CC, CT and TT in the oral squamous cell carcinoma (OSCC) patients were 72%, 22% and 6% respectively and for control group 88%, 12% and 0% respectively. CT and TT genotypes of miR149 polymorphism were found to be significantly associated with OSCC (p = 0.05 and 0.07). Conclusions: Our study suggests that miR-196a2C>T and miR-149C>T polymorphisms may play crucial roles in the development of OSCC in South Indian subjects.

Keywords

miRNA;polymorphisms;oral squamous cell carcinoma;PCR-RFLP;genotypes;South India

References

  1. Ahn DH, Rah H, Choi YK, et al (2013). Association of the miR- 146aC>G, miR-149T>C, miR-196a2T>C, and miR-499A>G polymorphisms with gastric cancer risk and survival in the Korean population. Mol Carcinog, 52, 39-51.
  2. Akkiz H, Bayram S, Bekar A, et al (2011). A functional polymorphism in pre-microRNA-196a-2 contributes to the susceptibility of hepatocellular carcinoma in a Turkish population: a case-control study. J Viral Hepat, 18, 399-407. https://doi.org/10.1111/j.1365-2893.2010.01414.x
  3. Ambros V (2004). The functions of animal micro RNAs. Nature, 431, 350-5. https://doi.org/10.1038/nature02871
  4. Aqeilan RI, Calin GA, Croce CM (2010). miR-15a and miR-16-1 in cancer: discovery, function and future perspectives. Cell Death Differ, 17, 215-20. https://doi.org/10.1038/cdd.2009.69
  5. Bartel DP (2004). MicroRNAs: Genomics, Review Biogenesis, Mechanism, and Function. Cell, 116, 281-97. https://doi.org/10.1016/S0092-8674(04)00045-5
  6. Chu H, Wang M, Shi D, et al (2011). Hsa-miR-196a2 Rs11614913 polymorphism contributes to cancer susceptibility: evidence from 15 case-control studies. PLoS One, 6, 18108. https://doi.org/10.1371/journal.pone.0018108
  7. Chen H, Sun LY, Chen LL, et al (2012). A variant in microRNA- 196a2 is not associated with susceptibility to and progression of colorectal cancer in Chinese. Intern Med J, 42, 115-119. https://doi.org/10.1111/j.1445-5994.2011.02434.x
  8. Dou T, Wu Q, Chen X, et al (2010). A polymorphism of microRNA196a genome region was associated with decreased risk of glioma in Chinese population. J Cancer Res Clin Oncol, 136, 1853-9. https://doi.org/10.1007/s00432-010-0844-5
  9. Elango JK, Gangadharan P, Sumithra S, et al (2006). Trends of head and neck cancers in urban and rural India. Asian Pac J Cancer Prev, 7, 108-11.
  10. George GP, Gangwar R, Mandal RK, et al (2011). Genetic variation in microRNA genes and prostate cancer risk in North Indian population. Mol Biol Rep, 38, 1609-15. https://doi.org/10.1007/s11033-010-0270-4
  11. Gregory RI, Shiekhattar R (2005). MicroRNA biogenesis and cancer. Cancer Res, 65, 3509-3512. https://doi.org/10.1158/0008-5472.CAN-05-0298
  12. Huang GL, Lu Y, Pu XX, et al (2013). Association study between miR149 gene polymorphism and nasopharyngeal carcinoma. Biomed Rep, 1, 599-603. https://doi.org/10.3892/br.2013.97
  13. Hu Z, Chen J, Tian T, et al (2008). Genetic variants of miRNA sequences and non-small cell lung cancer survival. J Clin Invest, 118, 2600-8.
  14. Jazdzewski K, Murray EL, Franssila K, et al (2008). Common SNP in pre-miR-146a decreases mature miR expression and predisposes to papillary thyroid carcinoma. Proc Natl Acad Sci U S A, 105, 7269-74. https://doi.org/10.1073/pnas.0802682105
  15. Kwak PB, Iwasaki S, Tomari Y (2010). The microRNA pathway and cancer. Cancer Sci, 101, 2309-15. https://doi.org/10.1111/j.1349-7006.2010.01683.x
  16. Lewis, Benjamin P, Christopher B, et al (2005). Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets, Cell, 120, 15-20. https://doi.org/10.1016/j.cell.2004.12.035
  17. Lin T, Ding Z, Li N, et al (2011). 2-Tellurium-bridged $\beta$-cyclodextrin, a thioredoxinreductase inhibitor, sensitizes human breast cancer cells to TRAIL-induced apoptosis through DR5 induction and NF-kB suppression. Carcinogenesis, 32, 154-167. https://doi.org/10.1093/carcin/bgq234
  18. Liu Z, Li G, Wei S, et al (2010). Genetic variants in selected pre-mircroRNA genes and risk of squamous cell carcinoma of the head and neck. Cancer, 116, 4753-60. https://doi.org/10.1002/cncr.25323
  19. Lovat, Francesca, Nicola Valeri, Carlo M, Croce (2011). MicroRNAs in the pathogenesis of cancer. Seminars Oncolo, 38, 6.
  20. Okubo M, Tahara T, Shibata T, et al (2010). Association between common genetic variants in pre-microRNAs and gastric cancer risk in japanese population. Helicobacter, 15, 524-531. https://doi.org/10.1111/j.1523-5378.2010.00806.x
  21. Peng S, Kuang Z, Sheng C, et al (2010). Association of microRNA-196a-2 gene polymorphism with gastric cancer risk in a Chinese population. Dig Dis Sci, 55, 2288-93. https://doi.org/10.1007/s10620-009-1007-x
  22. Rai R, Kulkarni V, Saranath D (2004). Genome wide instability scanning in chewing-tobacco associated oral cancer using inter simple sequence repeat PCR. Oral Oncol, 40, 1033-9. https://doi.org/10.1016/j.oraloncology.2004.05.009
  23. Ryan BM, Robles AI, Harris CC (2010). Genetic variation in microRNA networks: the implications for cancer research. Nat Rev Cancer, 10, 389-402. https://doi.org/10.1038/nrc2867
  24. Salzman DW, Weidhaas JB (2013). SNPing cancer in the bud: MicroRNA and microRNA-target site polymorphisms as diagnostic and prognostic biomarkers in cancer. Pharmacol Ther, 137, 55-63. https://doi.org/10.1016/j.pharmthera.2012.08.016
  25. Sassen S, Miska EA, Caldas C (2008). MicroRNA: implications for cancer. Virchows Arch, 452, 1-10.
  26. Sheng Peng, Zhong shen Kuang, Chenyi Sheng, et al (2010). Association of miRNA 196a - 2 gene polymorphism with gastric cancer risk in a Chinese population. Dig Dis Sci, 55, 2288- 93. https://doi.org/10.1007/s10620-009-1007-x
  27. Shen J1, Ambrosone CB, DiCioccio RA, etal (2008). A functional polymorphism in the miR146a gene and age of familial breast/ovarian cancer diagnosis. Carcinogenesis, 29, 1963-1966. https://doi.org/10.1093/carcin/bgn172
  28. Tu HF, Liu CJ, Chang CL, et al. (2012) The Association between Genetic Polymorphism and the Processing Efficiency of miR-149Affects the Prognosis of Patients with Head and Neck Squamous Cell Carcinoma. PLoS ONE, 7, 51606. https://doi.org/10.1371/journal.pone.0051606
  29. Wang K, Guo H, Hu H, Xiong G, et al (2010). A functional variation in pre-microRNA-196a is associated with susceptibility of esophageal squamous cell carcinoma risk in Chinese Han. Biomarkers, 15, 614-8. https://doi.org/10.3109/1354750X.2010.505299
  30. Wang N, Li Y, Zhou RM, et al (2014). Hsa-miR-196a2 functional SNP is associated with the risk of ESCC in individuals under 60 years old. Biomarkers, 19, 43-48. https://doi.org/10.3109/1354750X.2013.866164
  31. Xu T, Zhu Y, Wei QK, et al (2008). A functional polymorphism in the miR-146a gene is associated with the risk for hepatocellular carcinoma. Carcinogenesis, 29, 2126-31. https://doi.org/10.1093/carcin/bgn195
  32. Xu Y, Liu L, Liu J, et al (2011). A potentially functional polymorphism in the promoter region of miR-34b/c is associated with an increased risk for primary hepatocellular carcinoma. Int J Cancer, 128, 412-417. https://doi.org/10.1002/ijc.25342
  33. Yang H, Dinney CP, Ye Y, et al (2008). Evaluation of genetic variants in microRNA-related genes and risk of bladder cancer. Cancer Res, 68, 2530-7. https://doi.org/10.1158/0008-5472.CAN-07-5991
  34. Yoon KA, Yoon H, Park S, et al (2012). The prognostic impact of microRNA sequence polymorphisms on the recurrence of patients with completely resected non-small cell lung cancer. J Thorac Cardio Vas cSurg, 144, 794-807. https://doi.org/10.1016/j.jtcvs.2012.06.030

Cited by

  1. The Association between miR-196a2 rs11614913 Polymorphism and Digestive System Cancer Risk: A Meta-Analysis of 34 Studies vol.06, pp.04, 2016, https://doi.org/10.4236/ojim.2016.64017
  2. gene network in tumor pathogenesis and progression vol.234, pp.1, 2018, https://doi.org/10.1002/jcp.27036