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Clinical Significance of the NQO1 C609T Polymorphism in Non Small Cell Lung Adenocarcinoma Patients

  • Masroor, Mirza (Biochemistry, Maulana Azad Medical College) ;
  • Jain, Amit (Biochemistry, Maulana Azad Medical College) ;
  • Javid, Jamsheed (Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, University of Tabuk) ;
  • Mir, Rashid (Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, University of Tabuk) ;
  • Prashant, Y (Biochemistry, Maulana Azad Medical College) ;
  • Imtiyaz, A (Biochemistry, Maulana Azad Medical College) ;
  • Mariyam, Z (Biochemistry, Maulana Azad Medical College) ;
  • Mohan, Anant (Department of Pulmonary Medicine and Sleep Disorder All India Institute of Medical Sciences) ;
  • Ray, PC (Biochemistry, Maulana Azad Medical College) ;
  • Saxena, Alpana (Biochemistry, Maulana Azad Medical College)
  • Published : 2015.12.03

Abstract

Background: NAD(P)H:quinone oxidoreductase 1 (NQO1) is part of the antioxidant defence system involved in detoxification. This study aimed to analyze the influence of NQO1 (C609T) genetic polymorphism in non small cell lung cancer (NSCLC)as a putative risk factor. Materials and Methods: Present study included 100 cases of NSCLC (adenocarcinoma) patients and 100 age and sex matched healthy controls. NQO1 (C609T) genotyping was performed by allele specific PCR for assessment of putative associations with clinical outcome and genotypes of. The association of the polymorphism with the survival of NSCLC patients' was analyzed by Kaplan-Meier method. Results: In Indian NSCLC (adenocarcinoma) patients increased risk of developing NSCLC was found to be associated with NQO1 609TT genotype [OR 3.68(0.90-14.98), RR 2.04(0.78-5.31)] for CT [OR 2.91(1.58-5.34), RR 1.74(1.23-2.44) p=0.0005 for CT], for CT+TT [ OR 3.26(1.82-5.82), RR 1.87(1.34-2.61) p<0.0001 for CT+TT]. A significant difference (p=0.0009) was observed in genotype distribution among cases and healthy controls. Patients with CT+TT genotype exhibited a significant poor overall survival compared with patients displaying homozygous CC genotype (p=0.03) and when survival independently compared with CC, TT and CT genotype was also found to be significantly associated (p=0.02). Overall median survival times were CT 6.0 months, TT 8.2 months, and CT + TT (6.4 months)]. Conclusions: The present study revealed that NQO1 CT, TT and CT+TT genotypes may be associated with clinical outcome and risk of developing NSCLC in the Indian population.

Keywords

NSCLC patients;adenocarcioma;NQO1 gene (C609T) polymorphism;AS-PCR

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