Protein Expression of Stromelysin-2 in Head and Neck Squamous Cell Carcinomas

  • Kadeh, Hamideh (Oral and Dental Disease Research Center, Dept. of Oral and Maxillofacial Pathology, School of Dentistry) ;
  • Heydari, Fatemeh (Department of Pathology, School of Medicine, Zahedan University of Medical Sciences) ;
  • Saravani, Shirin (Oral and Dental Disease Research Center, Dept. of Oral and Maxillofacial Pathology, School of Dentistry) ;
  • Ghodsi, Iman Najafi (General Dentist)
  • Published : 2015.12.03


Background: Some matrix metalloproteinases (MMPs) are involved in invasion and metastasis of head and neck squamous cell carcinoma (HNSCC). However, there are few studies on association between stromelysin-2 (ST-2) and invasive behavior of HNSCC. The purpose of this study was to investigate Stromelysin-2 expression by immunohistochemistry. Materials and Methods: This study was conducted on 81 specimens, including 61 HNSCC and 20 non neoplastic epithelium. Sections with 5 micron thickness were prepared and stained with immunohistochemistry technique. Then expression of ST-2 was evaluated according to percentage of stained cells and intensity of staining. Data were analyzed by SPSS (V.21) using Kruskal-Wallis and Tukey tests (P<0.05). Results: The 61 HNSCC specimens were grades I 36.1%, II 34.4% and III 29.5%. The level of ST-2 expressions were moderate (++) and intensive (+++) in 21.3% and 78.7% of tumors, respectively. The ST-2 expression level was only significant between the tumors with grade I and grade III (P=0.016). Tumors presented ST-2 expression with staining intensity of mild 6.6%, moderate 26.2% and strong 67.2%. Staining intensity of ST-2 in grade I tumors was significantly lower than grade II and grade III (P<0.05), and there was no significant difference between grades II and III (P=0.99). Conclusions: According to this study, the expression of ST-2 is associated with histopathological grade and tumor differentiation in HNSCCs.


Head and neck squamous cell carcinoma;immunohistochemistry;stromelysin-2


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