Presence of Human Papillomavirus DNA in Colorectal Cancer Tissues in Shiraz, Southwest Iran

  • Mahmoudvand, Shahab (Department of Bacteriology & Virology, School of Medicine, Shiraz University of Medical Sciences) ;
  • Safaei, Akbar (Department of Pathology, School of Medicine, Shiraz University of Medical Sciences) ;
  • Erfani, Nasrollah (Cancer immunology group, Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences) ;
  • Sarvari, Jamal (Department of Bacteriology & Virology, School of Medicine, Shiraz University of Medical Sciences)
  • 발행 : 2015.12.03


Background: Colorectal cancer is one of the most common cancers worldwide. Viruses including human papillomavirus (HPV) have been reported to be associated with different cancers but any association with colorectal cancers remains controversial. Aim: To evaluate any association between HPV infection and adenocarcinoma of the colon and adenomatous polyps. Materials and Methods: Paraffin-embedded tissue specimens of 70 colorectal adenocarcinomas, 70 colorectal adenomatous polyps, and 70 colorectal normal tissues were subjected to DNA extraction. The quality of the extracted DNA was confirmed by amplification of a ${\beta}$-globin fragment using polymerase chain reaction (PCR). PCR using specific primers were performed to detect HPV DNA. Specific primers targeting the E6 region of the HPVs 16 and 18 were used for genotyping. Results: HPV DNA was detected in 2 (2.85%) out of 70 adenocarcinoma colorectal tissues and 4 (5.71 %) out of 70 adenomatous colorectal tissues. All normal colorectal tissues were negative for HPV DNA. HPV-16 was the most predominant genotype (5 sample) followed by HPV-18 (4 sample). Despite the above observations, statistical analyses indicated no significant differences in the frequencies of HPV positive subjects between the cancerous and normal samples. Conclusions: Although the differences observed in the frequencies of HPV positive cases in our study was not significant relative to those of control subjects, the fact of 6 positive samples among cancerous tissues, may still suggest a role of HPV in colorectal carcinogenesis. The study collectively indicated that some colorectal cancerous tissues are infected with high risk HPV genotype. The findings merit more investigation.


Colorectal cancer;HPV;adenocarcinoma;adenomatous polyp


연구 과제 주관 기관 : Shiraz University of Medical Sciences


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피인용 문헌

  1. Seroepidemiological Evaluation of High-Risk Human Papillomavirus Types Among Married and Unmarried Iranian Women in Tehran, Iran vol.In Press, pp.In Press, 2016,