- Volume 16 Issue 17
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JAK-2 V617F Mutational Analysis in Primary Idiopathic Myelofibrosis: Experience from Southern Pakistan
- Sultan, Sadia (Department of Hematology and Blood Bank, Liaquat National Hospital and Medical College) ;
- Irfan, Syed Mohammed (Department of Hematology and Blood Bank, Liaquat National Hospital and Medical College)
- Published : 2015.12.03
Background: Primary idiopathic myelofibrosis (PMF) is a clonal Ph-chromosome negative myeloproliferative neoplasm characterized by dysregulated kinase signaling and release of abnormal cytokines. In the recent past, following JAK2 V617F mutation invention, important revolution has been made in the molecular diagnostic biology of this disease. The rational of this study was to determine the mutational status of JAK2 V617F in Pakistan patients with PMF. Materials and Methods: In this cross sectional study, 20 patients with PMF were enrolled from January 2011 to December 2014. Diagnosis was based on WHO criteria for PMF. All patients were screened for G-T point mutation (V617F) in the JAK2 gene on chromosome 9 by allele specific PCR. Results: The mean age was
Primary idiopathic myelofibrosis;JAK-2 V617F;mutational analysis;Pakistan
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