- Volume 16 Issue 17
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DNA Ploidy and S-phase Fraction Analysis in Paediatric B-cell Acute Lymphoblastic Leukemia Cases: a Tertiary Care Centre Experience
- Kumar, Banothu Kiran (Department of Pediatrics, All India Institute of Medical Sciences) ;
- Bhatia, Prateek (Pediatric Hemato-Oncology Unit, Department of Paediatrics, Post Graduate Institute of Medical Education and Research (PGIMER)) ;
- Trehan, Amita (Pediatric Hemato-Oncology Unit, Department of Paediatrics, Post Graduate Institute of Medical Education and Research (PGIMER)) ;
- Singh, Ajit Pal (Pediatric Hemato-Oncology Unit, Department of Paediatrics, Post Graduate Institute of Medical Education and Research (PGIMER)) ;
- Kaul, Deepak (Pediatric Hemato-Oncology Unit, Department of Paediatrics, Post Graduate Institute of Medical Education and Research (PGIMER)) ;
- Bansal, Deepak (Pediatric Hemato-Oncology Unit, Department of Paediatrics, Post Graduate Institute of Medical Education and Research (PGIMER))
- Published : 2015.12.03
DNA ploidy is an important prognostic parameter in paediatric B-ALL, but the significance of the S-phase fraction is unclear. In present study, DNA ploidy was assessed in 40 pediatric B-ALL cases by flow cytometry. The DI (DNA index) and percentage of cells in S-phase were calculated using Modfit software. Aneuploidy was noted in 26/40 (65%) cases. A DI of 1.10-1.6 (hyperdiploidy B) was noted in 20/40 (50%) and 6/40 (15%) had a DI>1.60 (triploid and tetraploid range). Some 14/40 (35%) cases had a diploid DI between 0.90-1.05. None of the cases had a DI <0.90 (hypodiploid) or in the 1.06-1.09 (hyperdiploid A) range. The mean S-phase fraction was 2.6%, with 24/40 (60%) having low and 16/40 (40%) high S-phase fractions. No correlation was noted with standard ALL risk and treatment response factors with DI values or S-phase data, except for a positive correlation of low S-phase with high NCI risk category (p=0.032). Overall frequency of hyperdiploidy in our cohort of B-ALL patients was very high (65%). No correlation between hyperdiploidy B and low TLC or common B-phenotype was observed in our study as 42% cases with DI 1.10-1.6 had TLC>
ALL;DNA-index;ploidy;S-phase fraction;paediatric leukemia cases
- Amare P, Gladstone B, Varghesse C, Pai S, Advani S (1999). Clinical significance of cytogenetic findings at diagnosis and in remission in childhood and adult Acute Lymphoblastic leukemia: Experience from India. Cancer Genet Cytogenet, 110, 44-53. https://doi.org/10.1016/S0165-4608(98)00179-4
- Basu O, Zolzer F, Uma DP (2009). DNA Ploidy - A Prognostic Factor of Acute Lymphoblastic Leukemia (ALL) in Childhood. Asian J Exp Sci, 23, 33-8.
- Faderl S, Kantarjian HM, Talpaz M, Estrov Z (1998). Clinical significance of cytogenetic abnormalities in adult acute lymphoblastic leukemia. Blood, 91, 3995-4019.
- Foud M AT, Maha R AW, Hala A AA, Amal A Z, Saed S E (2002). Flow cytometric assessment of nuclear DNA ploidy and proliferative activity in acute lymphoblastic leukemia. J Nat Egyptian Cancer Inst, 14, 303-10.
- Hilden JM, Dinndorf PA, Meerbaum SO, et al (2006). Children's Oncology Group. Analysis of prognostic factors of acute lymphoblastic leukemia in infants: report on CCG 1953 from the Children's Oncology Group. Blood, 108, 441-51. https://doi.org/10.1182/blood-2005-07-3011
- Jena RK, Patnaik SC, Sahu GR, Ray B, Swain K (2002). Secondary chromosomal abnormalities in Acute lymphoblastic Leukemia. Caryologia, 55, 349-55. https://doi.org/10.1080/00087114.2002.10797886
- Kaaijk P, Kaspers GJL, Van-Wering ER, Broekema GJ, Loonen AH, Hahlen K (2003). Cell proliferation is related to in vitro drug resistance in childhood acute leukaemia. Br J Cancer. 88, 775-81. https://doi.org/10.1038/sj.bjc.6600787
- Khalifa AS, El-Kardani TA, Faeyk MH (1997). Flowcytometric study childhood acute leukemia immunophenotype and DNA analysis. Egypt J Haematol, 22, 1-23.
- Lustosa de Sousa DW, de Almeida Ferreira FV, Cavalcante Felix FH, de Oliveira Lopes MV (2015). Acute lymphoblastic leukemia in children and adolescents: prognostic factors and analysis of survival. Rev Bras Hematol Hemoter. 37, 223-9. https://doi.org/10.1016/j.bjhh.2015.03.009
- Mullighan CG, Su X, Zhang J, et al (2009). Children's Oncology Group. Deletion of IKZF1 and prognosis in acute lymphoblastic leukemia. N Engl J Med, 360, 470-80. https://doi.org/10.1056/NEJMoa0808253
- N Chaudhary, A Borker (2013). Significance of karyotyping in childhood acute lymphoblastic leukemia. Pediatric Oncall Journal, 10, 39-41.
- Olah E, Balogh E, Pajor L, Jakab Z (2011). Ten-year experiences on initial genetic examination in Childhood Acute Lymphoblastic Leukemia in Hungary (1993-2002): Technical Approaches and Clinical Implementation. Pathol Oncol Res, 17, 81-90. https://doi.org/10.1007/s12253-010-9286-2
- Raimondi SC, Behm FG, Roberson PK, et al (1988). Cytogenetics of childhood T-cell leukemia. Blood, 72, 1560-6.
- Raimondi SC, Zhou Y, Mathew S, et al (2003). Reassessment of the prognostic significance of hypodiploidy in pediatric patients with acute lymphoblastic leukemia. Cancer, 98, 2715-22. https://doi.org/10.1002/cncr.11841
- Shaikh MS, Ali SS, Khurshid M, Fadoo Z (2014). Chromosomal abnormalities in Pakistani children with acute lymphoblastic leukemia. Asia Pac J Cancer Prev, 15, 3907-9. https://doi.org/10.7314/APJCP.2014.15.9.3907
- Somnath P, Rajlaxmi S, Pranati M, Rupa Das, Sukumar C, Raghumani M (2011). Cytogenetic profile of pediatric acute lymphoblastic leukemia (ALL): Analysis of 31 cases with review of literature. Caryologia, 64, 33-41. https://doi.org/10.1080/00087114.2011.10589762
- Woo JS, Alberti MO, Tirado CA (2014). Childhood B-acute lymphoblastic leukemia: a genetic update. Experimental Hematology & Oncology, 3, 1-14. https://doi.org/10.1186/2162-3619-3-1
- XM Wang (2014). Advances and issues in flow cytometric detection of immunophenotypic changes and genomic rearrangements in acute pediatric leukemia. Transl Pediatr, 3, 149-55.
- Yeoh EJ, Ross ME, Shurtleff SA, et al (2002). Classification, subtype discovery and prediction of outcome in pediatric acute lymphoblastic leukemia by gene expression profiling. Cancer Cell, 1, 133-43. https://doi.org/10.1016/S1535-6108(02)00032-6
- Simultaneous use of multiplex ligation-dependent probe amplification assay and flow cytometric DNA ploidy analysis in patients with acute leukemia pp.15524949, 2017, https://doi.org/10.1002/cyto.b.21523
- FxCycle™ Based Ploidy Correlates with Cytogenetic Ploidy in B-Cell Acute Lymphoblastic Leukemia and Is Able to Detect the Aneuploid Minimal Residual Disease Clone pp.15524949, 2019, https://doi.org/10.1002/cyto.b.21765