α-Tocopherol을 함유한 Nanostructured Lipid Carriers의 특성과 안정화 효과

Jun, Yoon Kyung;Lim, Yoon Mi;Jin, Byung Suk

  • 투고 : 2015.07.28
  • 심사 : 2015.10.20
  • 발행 : 2015.12.10


지용성 토코페롤(${\alpha}$-tocopherol)의 산화 안정성을 높이기 위하여 nanostructured lipid carrier (NLC)에 봉입을 시도하였다. 먼저 구성 성분과 혼합 비율을 달리한 여러 NLC를 만들고 각각의 특성을 살펴보았다. 고체 지질로 세틸 팔미테이트 또는 글리세릴 모노스테아레이트를 사용했을 때는 안정한 상태의 NLC 입자가 만들어지지만 스테아린 산으로 만든 NLC에서는 상분리가 일어났다. NLC 입자는 수백 나노미터 크기로 만들어지는데 지질대비 용매의 비율이 높을수록 입자크기가 작게 나타났다. 고급 지방알콜인 옥틸도데칸올을 고체 지질에 첨가하면 결정화도가 감소하면서 지질 매트릭스의 배열규칙성 이 약해짐을 DSC (Differential Scanning Calorimetry) 열차트와 anisotropy 측정을 통해서 확인하였다. 고온($45^{\circ}C$) 또는 UV 빛이 조사되는 조건에서 토코페롤이 NLC에 봉입되어 있으면 용액이나 에멀젼 상태로 있는 것에 비해 산화 안정성이 크게 향상됨을 DPPH 테스트와 과산화물가 측정으로 확인하였다.


nanostructured lipid carrier;NLC;${\alpha}$-tocopherol;oxidative stability


  1. R. H. Muller, M. Radtke, and S. A. Wissing, Solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC) in cosmetic and dermatological preparations, Adv. Drug Delivery Rev., 54(1), S131-S155 (2002).
  2. E. J. An, C. K. Kang, J. W. Kim, and B. S. Jin, Lipid-based Vesicles as Transdermal Delivery System, KIC News, 13(4), 24-34 (2010).
  3. R. H. Müller, R. D. Petersen, A. Hommoss, and J. Pardeike, Nanostructured lipid carriers (NLC) in cosmetic dermal products, Adv. Drug Delivery Rev., 59(6), 522-530 (2007).
  4. H. Bunjes and K. Westesen, and M. H. J. Koch, Crystallization tendency and polymorphic transitions in truglyceride nanoparticles, Int. J. Pharm, 129, 159-173 (1996).
  5. J. Pardeike, A. Hommoss, and R. H. Muller, Lipid nanoparticles (SLN, NLC) in cosmetic and pharmaceutical dermal products, Inter. J. Pharm., 366, 170-184 (2009).
  6. J. Varshosaz, S. Eskandari, and M. Tabakhian, Production and optimization of valproic acid nanostructured lipid carriers by the Taguchi design, Pharm. Dev. and Tech., 15(1), 89-96 (2010).
  7. C. W. Pouton, Lipid formulations for oral administration of drugs: non-emulsifying, self-emulsifying and 'self-microemulsifying' drug delivery systems, Eur. J. Pharm. Sci., 11(2), S93-S98 (2000).
  8. M. P. Lupo, Antioxidants and vitamins in cosmetics, Clinics in Dermatology, 19, 467-473 (2001).
  9. D. Perrenoud, H. P. Homberger, P. C. Auderset et al., An epidemic outbreak of papular and follicular contact dermatitis to tocopheryl linoleate in cosmetics, Dermatology, 189, 225-233 (1994).
  10. B. Palmieri, G. Gozzi, and G. Palmieri, Vitamin E added silicone gel sheets for treament of hypertrophic scars and keloids, Inter. J. Dermatology, 34(7), 506-509 (1995).
  11. W. H. Sebrell, The Vitamins: Chemistry, Physiology, Pathology, Methods, Academic Press, 3, 233-248 (2013).
  12. J. R. Lakowicz, Principles of fluorescence spectroscopy, Springer Science & Business Media, 3rd edition, 298-299 (2013).
  13. B. S. Jin, H. C. Kang, D. I. Kim, K. H. Lee, and Y. H. Bae, Synthesis of poly(cystine bisamide)-PEG block copolymers grafted with 1-(3-aminopropyl)imidazole and their phase transition behaviors, Polym. Adv. Technol., 19, 1558-1565 (2008).
  14. F. Q. Hu, S. P. Jiang, Y. Z. Du, H. Yuan, Y. Q. Ye, and S. Zeng, Preparation and characterization of stearic acid nanostructured lipid carriers by solvent diffusion method in an aqueous system, Colloids and Surf. B: Biointerfaces, 45, 167-173 (2005).
  15. M. A. Schubert and C. C. Muller-Goymann, Solvent injection as a new approach for manufacturing lipid nanoparticles-evaluation of the method and process parameters, Eur. J. Pharm. Biopharm, 55, 125-131 (2003).
  16. V. Teeranachaideekul, E. B. Souto, V. B. Junyaprasert, and R. H. Muller, Cetyl palmitate-based NLC for topical delivery of Coenzyme Q10-Development, physicochemical characterization and in vitro release studies, Eur. J. Pharm. and Biopharm., 67, 141-148 (2007).
  17. W. Mehnert and K. Mader, Solid lipid nanoparticles: production, characterization and applicatipons, Adv. Drug Deliv. Rev., 47, 165-196 (2001).
  18. C. M. Sabliov, C. Fronczek, C. E. Astete, M. Khachaturyan, L. Khachatryan, and C. Leonardi, Effects of temperature and UV Light on degradation of ${\alpha}$-Tocopherol in free and dissolved form, J. Am. Oil Chem. Soc., 86, 895-902 (2009).
  19. F. H. Lin, J. Y. Lin, R. D. Gupta, J. A. Tournas, J. A. Burch, M. A. Selim, N. A. Monteiro-Riviere, J. M. Grichnik, J. Zielinski, and S. R. Pinnell, Ferulic acid stabilizes a solution of vitamins C and E and doubles its photoprotection of skin, The Journal of Investigative Dermatology, 125, 826-832 (2005).
  20. S. E. Jeon, S. J. Kim, and B. S. Jin, Stabilization of Ascorbic acid by Hydroxycinnamic Acid Incorporation and BGsome Encapsulation, J. of Kor. Oil Chemists' Soc., 29(2), 348-355 (2012).


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