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A Candidate Single Nucleotide Polymorphism in the 3' Untranslated Region of Stearoyl-CoA Desaturase Gene for Fatness Quality and the Gene Expression in Berkshire Pigs

  • Lim, Kyu-Sang (College of Life Sciences and Biotechnology, Korea University) ;
  • Kim, Jun-Mo (College of Life Sciences and Biotechnology, Korea University) ;
  • Lee, Eun-A (College of Life Sciences and Biotechnology, Korea University) ;
  • Choe, Jee-Hwan (College of Life Sciences and Biotechnology, Korea University) ;
  • Hong, Ki-Chang (College of Life Sciences and Biotechnology, Korea University)
  • Received : 2014.07.16
  • Accepted : 2014.09.30
  • Published : 2015.02.01

Abstract

Fatness qualities in pigs measured by the amount of fat deposition and composition of fatty acids (FAs) in pork have considerable effect on current breeding goals. The stearoyl-CoA desaturase (SCD) gene plays a crucial role in the conversion of saturated FAs into monounsaturated FAs (MUFAs), and hence, is among the candidate genes responsible for pig fatness traits. Here, we identified a single nucleotide polymorphism (SNP, $c.^*2041T$ >C) in the 3' untranslated region by direct sequencing focused on coding and regulatory regions of porcine SCD. According to the association analysis using a hundred of Berkshire pigs, the SNP was significantly associated with FA composition (MUFAs and polyunsaturated FAs [PUFAs]), polyunsaturated to saturated (P:S) FA ratio, n-6:n-3 FA ratio, and extent of fat deposition such as intramuscular fat and marbling (p<0.05). In addition, the SNP showed a significant effect on the SCD mRNA expression levels (p = 0.041). Based on our results, we suggest that the SCD $c.^*2041T$ >C SNP plays a role in the gene regulation and affects the fatness qualities in Berkshire pigs.

Keywords

Stearoyl-CoA Desaturase [SCD];Polymorphism;Gene Expression;Fatness Quality;Berkshire

Acknowledgement

Supported by : Rural Development Administration

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