Nutritional Value of Rice Bran Fermented by Bacillus amyloliquefaciens and Humic Substances and Its Utilization as a Feed Ingredient for Broiler Chickens

  • Supriyati, Supriyati (Indonesian Research Institute for Animal Production) ;
  • Haryati, T. (Indonesian Research Institute for Animal Production) ;
  • Susanti, T. (Indonesian Research Institute for Animal Production) ;
  • Susana, I.W.R. (Indonesian Research Institute for Animal Production)
  • Received : 2014.01.16
  • Accepted : 2014.06.09
  • Published : 2015.02.01


An experiment was conducted to increase the quality of rice bran by fermentation using Bacillus amyloliquefaciens and humic substances and its utilization as a feed ingredient for broiler chickens. The experiment was carried out in two steps. First, the fermentation process was done using a completely randomized design in factorial with 16 treatments: i) Dosage of B.amyloliquefaciens ($2.10^8cfu/g$), 10 and 20 g/kg; ii) Graded levels of humic substances, 0, 100, 200, and 400 ppm; iii) Length of fermentation, three and five days. The results showed that the fermentation significantly (p<0.05) reduced crude fiber content. The recommended conditions for fermentation of rice bran: 20 g/kg dosage of inoculums B. amyloliquefaciens, 100 ppm level of humic substances and three days fermentation period. The second step was a feeding trial to evaluate the fermented rice bran (FRB) as a feed ingredient for broiler chickens. Three hundred and seventy-five one-day-old broiler chicks were randomly assigned into five treatment diets. Arrangement of the diets as follows: 0%, 5%, 10%, 15%, and 20% level of FRB and the diets formulation based on equal amounts of energy and protein. The results showed that 15% inclusion of FRB in the diet provided the best bodyweight gain and feed conversion ratio (FCR) values. In conclusion, the nutrient content of rice bran improved after fermentation and the utilization of FRB as a feed ingredient for broiler chickens could be included up to 15% of the broiler diet.


Broiler Chickens;Crude Fiber;Fermentation;Performance;Rice Bran


  1. AOAC. 2005. Official Methods of Analysis. 18th edn. Association of Official Analytical Chemists, Gaithersburg, MD, USA.
  2. An, B. K., B. L. Cho, S. J. You, H. D. Paik, H. I. Chang, S. W. Kim, C. W. Yun, and C. W. Kang. 2008. Growth performance and antibody response of broiler chicks fed yeast derived $\beta$-glucan and single-strain probiotics. Asian Australas. J. Anim. Sci. 21:1027-1032.
  3. Anonymous. 2012. Benefits of Bacillus amyloliquefaciens probiotic in broilers. Norel Animal Production. Technical Bulletin No 15.
  4. AOAC. 2000. Official Methods of Analysis. 17th edn. Association of Official Analytical Chemists, Arlington, VA, USA.
  5. AOAC International. 2012. Guidelines for Standard Method Performance Requirements. AOAC Official Methods of Analysis, 19th Ed. Appendix F, Gaithersburg, MD, USA.
  6. Bidura, I. G. N. G., I. G. Mahardika, I. P. Suyadnya, I. B. Gaga Partama, I. G. L. Oka, D. P. M. A. Candrawati, and I. G. A. I. Aryani. 2012. The implementation of Saccharomyces spp. n-2 isolate culture (isolation from traditional yeast culture) for improving feed quality and performance of male Bali duckling. Agric. Sci. Res. J. 2:486-492.
  7. Campbell, R. C. 1967. Statistics for Biologist. Cambridge University Press, Cambridge, UK.
  8. Gallinger, C. I., D. M. Suarez, and A. Irazusta. 2004. Effects of rice bran inclusion on performance and bone mineralization in broiler chicks. J. Appl. Poult. Res. 13:183-190.
  9. Chae, B. J., K. H. Lee, and S. K. Lee. 2002. Effects of feeding rancid rice bran on growth performance and chicken meat quality in broiler chicks. Asian Australas. J. Anim. Sci. 15:266-273.
  10. Diaz, D. 2008. Safety and efficacy of $Ecobiol^{(R)}$ (Bacillus amyloliquefaciens) as feed additive for chickens for fattening. EFSA J. 773:2-13.
  11. El-Husseiny, O. M, A. G. Abdallah, and K. O. Abdel-Latif. 2008. The influence of biological feed additives on broiler performance. Int. J. Poult. Sci. 7:862-871.
  12. Gonzalez-Alvarado, J. M., E. Jimenez-Moreno, R. Lazaro, and G. G. Mateos. 2007. Effect of type and cereal, heat processing of the cereal, and inclusion of fiber in the diet on productive performance and digestive traits of broilers. Poult. Sci. 86:1705-1715.
  13. Gonzalez-Alvarado, J. M., E. Jimenez-Moreno, D. G. Valenzia, R. Lazaro, and G. G. Mateos. 2008. Effect of fiber source and heat processing of the cereal on the development and pH of gastrointestinal tract of broilers fed diets based on corn or rice. Poult. Sci. 87:1779-1795.
  14. Huck, T. A., N. Porter, and M. E. Bushell. 1991. Effect of humates on microbial activity. Gen. Microbiol. 137:2321-2329.
  15. Islam, K. M. S., A. Schuhmacher, and J. M. Gropp. 2005. Humic acid substances in animal agriculture. Pakistan J. Nutr. 4:126-134.
  16. Jimenez-Moreno, E., J. M. Gonzalez-Alvarado, D. Gonzalez-Sanchez, R. Lazaro, and G. G. Mateos. 2010. Effect of type and particle size of dietary fiber on growth performance and digestive traits of broiler from 1 to 21 days of age. Poult. Sci. 89:2197-2212.
  17. Karaoglu, M., M. Macit, N. Esenbuga, H. Durdag, L. Turgut, and O. C. Bilgin. 2004. Effect of suplemental humate at different levels on the growth performance, slaughter and carcass traits of broilers. Int. J. Poult. Sci. 3:406-410.
  18. Kubad, R. C., K. Singh, K. Saxena, and K. Eriksson. 1997. Microorganism as alternative source protein. Nutr. Rev. 55:65-75.
  19. Kompiang, I P., A. P. Sinurat, T. Purwadaria, J. Darma, and Supriyati. 1995. Cassapro in broiler rations: Interaction with rice bran. Indonesian J. Anim. Vet. Sci. 1:86-88.
  20. Kompiang, I P., D. Zaenuddin, and Supriyati. 2002. Effect of supplementation Bacillus apiarius or Torulaspora delbrueckii on broilers performances. Indonesian J. Anim. Vet. Sci. 7:139-143.
  21. Kompiang, I P. and Supriyati. 2007. Effect of humic acid on broilers performance. Indonesian J. Anim. Vet. Sci. 12:6-9.
  22. Mateos, G. G., R. Lazaro, and M. I. Gracia. 2002. The feasibility of using nutritional modifications to replace drugs in poultry feeds. J. Appl. Poult. Res. 11:437-452.
  23. Melegy, T., N. F. Khaled, R. El-Bana, and H. Abdellatif. 2011. Effect of dietary supplementation of Bacillus subtilis PB6 (ClostatTM) on performance, immunity, gut health ang carcass traits in broilers. J. Am. Sci. 7:891-898.
  24. Mirnawati, Y. Rizal, Y. Marlida, and I. P. Kompiang. 2010. The role of humic acid in palm kernel cake fermented by Aspergillus niger for poultry ration. Pakistan J. Nutr. 9:182-185.
  25. National Research Council. 1994. Nutrient Requirements of Poultry. 9th Ed. National Academy Press, Washington, DC, USA.
  26. Olomu, J. M. and S. A. Offiong. 1980. The effects of different protein and energy levels and time of change from starter to finisher ration on the performance of broiler chickens in the tropics. Poult. Sci. 59:828-835.
  27. Priest, F. G., M. Goodfellow, L. A. Shute, and R. C. W. Berkeley. 1987. Bacillus amyloliquefaciens sp. nov., nom. rev. Int. J. Systm. Bacteriol. 37:69-71.
  28. SAS Institute Inc. 2002. SAS/STAT User's Guide. Version 9.1. SAS Institute Inc., Cary, NC, USA.
  29. Wizna, H. Abbas, Y. Rizal, A. Dharma, and I P. Kompiang. 2007. Selection and identification of cellulase-producing bacteria isolated from the litter of mountain and swampy forest. J. Microbiol. Indonesia 1:135-139.
  30. Supriyati and I P. Kompiang. 2002. Change of nutrient composition of fermented cassava skin tubers and its utilization in broiler rations. Indonesian J. Anim. Vet. Sci. 7:150-154.
  31. Tangendjaja, B. 1993. Effect of fermacto upon the utilization of broiler diets containing normal and high levels of rice bran. Ilmu dan Peternakan 7:16-19.
  32. Tirajoh, S., W. G. Piliang, and P. P. Ketaren. 2010. The supplementation of fiber degrading enzymes and phytase in poultry diet on the performance of broiler chickens. Indonesian J. Anim. Vet. Sci. 15:40-46.
  33. Wizna, H. Abbas, Y. Rizal, A. Dharma, and I. P. Kompiang. 2009. Improving the quality of tapioca by-products (onggok) as poultry feed through fermentation by Bacillus amyloliquefaciens. J. Appl. Ind. Biotechnol. Trop. Reg. 2:1-5.
  34. Wizna, H. Abbas, Y. Rizal, A. Djulardi, and H. Muis. 2012. The effect of supplementation of micro nutrient on nutrient rice bran which fermented by Bacillus amyloliquefaciens. Pakistan J. Nutr. 11:439-443.

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