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Interaction between Leptospiral Lipopolysaccharide and Toll-like Receptor 2 in Pig Fibroblast Cell Line, and Inhibitory Effect of Antibody against Leptospiral Lipopolysaccharide on Interaction

  • Guo, Yijie (Laboratories of Animal Microbiology, Graduate School of Agricultural Science, Tohoku University) ;
  • Fukuda, Tomokazu (Laboratory of Animal Breeding and Genetics, Agricultural Science, Tohoku University) ;
  • Nakamura, Shuichi (Laboratory of Applied Physics, Graduate School of Engineering, Tohoku University) ;
  • Bai, Lanlan (Laboratories of Animal Microbiology, Graduate School of Agricultural Science, Tohoku University) ;
  • Xu, Jun (Laboratories of Animal Microbiology, Graduate School of Agricultural Science, Tohoku University) ;
  • Kuroda, Kengo (Laboratories of Animal Microbiology, Graduate School of Agricultural Science, Tohoku University) ;
  • Tomioka, Rintaro (Laboratories of Animal Microbiology, Graduate School of Agricultural Science, Tohoku University) ;
  • Yoneyama, Hiroshi (Laboratories of Animal Microbiology, Graduate School of Agricultural Science, Tohoku University) ;
  • Isogai, Emiko (Laboratories of Animal Microbiology, Graduate School of Agricultural Science, Tohoku University)
  • Received : 2014.06.13
  • Accepted : 2014.08.29
  • Published : 2015.02.01

Abstract

Leptospiral lipopolysaccharide (L-LPS) has shown potency in activating toll-like receptor 2 (TLR2) in pig fibroblasts (PEFs_NCC1), and causes the expression of proinflammatory cytokines. However, the stimulation by L-LPS was weak eliciting the function of TLR2 sufficiently in pig innate immunity responses during Leptospira infection. In this study, the immune response of pig embryonic fibroblast cell line (PEFs_SV40) was investigated and was found to be the high immune response, thus TLR2 is the predominate receptor of L-LPS in pig cells. Further, we found a strategy using the antibody against L-LPS, to prevent L-LPS interaction with TLR2 in pig cells which could impact on immune activation.

Keywords

Antibody Against Leptospiral Lipopolysaccharide;Cytokines;Leptospiral Lipopolysaccharide;Pig Fibroblast Cell Line;Toll-like Receptor 2

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