- Volume 55 Issue 4
DOI QR Code
Therapeutic effect of oregano essential oil on subclinical bovine mastitis caused by Staphylococcus aureus and Escherichia coli
- Cho, Byung-Wook ;
- Cha, Chun-Nam ;
- Lee, Soo-Mi ;
- Kim, Mee-Jeong ;
- Park, Ju-Yeon ;
- Yoo, Chang-Yeol ;
- Son, Song-Ee ;
- Kim, Suk ;
- Lee, Hu-Jang
- Received : 2015.11.13
- Accepted : 2015.12.24
- Published : 2015.12.31
Oregano (Origanum vulgare) is an aromatic herb belonging to the Lamiaceae family. This study evaluated the therapeutic effect of oregano essential oil (OEO) on clinical bovine mastitis caused by Staphylococcus (S.) aureus and/or Escherichia (E.) coli. Thirty-two quarters from 18 lactating cows with subclinical mastitis were selected from a dairy farm and were divided into 4 groups (8 quarters/group): negative control (CON), positive control (GEN) and two OEO-treated groups (OEO-1 and OEO-2). Each group was intramammarily treated with saline, 10 g/tube of gentamicin ointment, and single and double doses of 0.9 mL OEO ointment twice a day for three days, respectively. After the treatments, physical udder conditions were greatly improved in GEN and OEO groups. In CON group, somatic cell counts (SCCs), number of white blood cells (WBC) and bacteria were increased. However, in OEO groups, SCCs and number of WBC were significantly decreased and S. aureus and E. coli were not detected in milk as compared with those before the treatments as well as GEN group. These results suggest that OEO may be a useful alternative to antibiotics for the control of subclinical bovine mastitis caused by S. aureus and/or E. coli.
bovine mastitis;Escherichia coli;oregano essential oil;Staphylococcus aureus;therapeutic effect
- Ballou MA. Growth and development symposium: inflammation: role in the etiology and pathophysiology of clinical mastitis in dairy cows. J Anim Sci 2012, 90, 1466-1478. https://doi.org/10.2527/jas.2011-4663
- Baratta MT, Dorman HJD, Deans SG, Biondi DM, Ruberto G. Chemical composition, antimicrobial and antioxidative activity of laurel, sage, rosemary, oregano and coriander essential oils. J Essent Oil Res 1998, 10, 618-627. https://doi.org/10.1080/10412905.1998.9700989
- Bhasme PC, Kurjogi MM, Sanakal RD, Kaliwal RB, Kaliwal BB. In silico characterization of putative drug targets in Staphylococcus saprophyticus, causing bovine mastitis. Bioinformation 2013, 9, 339-344. https://doi.org/10.6026/97320630009339
- Burt S. Essential oils: their antibacterial properties and potential applications in foods-a review. Int J Food Microbiol 2004, 94, 223-253. https://doi.org/10.1016/j.ijfoodmicro.2004.03.022
- Cardozo VF, Lancheros CAC, Narciso AM, Valereto ECS, Kobayashi RKT, Seabra AB, Nakazato G. Evaluation of antibacterial activity of nitric oxide-releasing polymeric particles against Staphylococcus aureus and Escherichia coli from bovine mastitis. Int J Pharm 2014, 473, 20-29. https://doi.org/10.1016/j.ijpharm.2014.06.051
- De Oliveira AP, Watts JL, Salmon SA, Aarestrup FM. Antimicrobial susceptibility of Staphylococcus aureus isolated from bovine mastitis in Europe and the United States. J Dairy Sci 2000, 83, 855-862. https://doi.org/10.3168/jds.S0022-0302(00)74949-6
- De UK, Mukherjee R. Dynamics of milk leukocytes in response to a biological response modifier during bovine subclinical mastitis. Res Vet Sci 2013, 95, 352-357. https://doi.org/10.1016/j.rvsc.2013.06.010
- Dias RS, Eller MR, Duarte VS, Pereira AL, Silva CC, Mantovani HC, Oliveira LL, Silva E de A, De Paula SO. Use of phages against antibiotic-resistant Staphylococcus aureus isolated from bovine mastitis. J Anim Sci 2013, 91, 3930-3939. https://doi.org/10.2527/jas.2012-5884
- Fernandes JBC, Zanardo LG, Galvao NN, Carvalho IA, Nero LA, Moreira MAS. Escherichia coli from clinical mastitis: serotypes and virulence factors. J Vet Diagn Invest 2011, 23, 1146-1152. https://doi.org/10.1177/1040638711425581
- Frola ID, Pellegrino MS, Espeche MC, Giraudo JA, Nader-Macias ME, Bogni CI. Effects of intramammary inoculation of Lactobacillus perolens CRL1724 in lactating cows' udders. J Dairy Res 2012, 79, 84-92. https://doi.org/10.1017/S0022029911000835
- Hillerton JE, Berry EA. Treating mastitis in the cow - a tradition or an archaism. J Appl Microbiol 2005, 98, 1250-1255. https://doi.org/10.1111/j.1365-2672.2005.02649.x
- Kaithwas G, Mukerjee A, Kumar P, Majumdar DK. Linum usitatissimum (linseed/flaxseed) fixed oil: antimicrobial activity and efficacy in bovine mastitis. Inflammopharmacology 2011, 19, 45-52. https://doi.org/10.1007/s10787-010-0047-3
- Kasravi R, Bolourchi M, Farzaneh N, Seifi HA, Barin A, Hovareshti P, Gharagozlou F. Efficacy of conventional and extended intra-mammary treatment of persistent subclinical mastitis with cefquinome in lactating dairy cows. Trop Anim Health Prod 2011, 43, 1203-1210. https://doi.org/10.1007/s11250-011-9826-0
- Kim DH, Lim JJ, Lee JJ, Kim DG, Chang HH, Lee SJ, Lee YB, Chang DI, Lee HJ, Min WG, Kim SH, Oh KY, Kim S. Dehydrating and bacterial elimination effects of fecal dehydrating system for reducing bovine mastitis derived from environmental contamination. Korean J Vet Res 2009, 49, 257-263.
- Kutila T, Suojala L, Lehtolainen T, Saloniemi H, Kaartinen L, Tahti M, Seppala K, Pyorala S. The efficacy of bovine lactoferrin in the treatment of cows with experimentally induced Escherichia coli mastitis. J Vet Pharmacol Ther 2004, 27, 197-202. https://doi.org/10.1111/j.1365-2885.2004.00581.x
- Lambert RJW, Skandamis PN, Coote PJ, Nychas GJE. A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol. J Appl Microbiol 2001, 91, 453-462. https://doi.org/10.1046/j.1365-2672.2001.01428.x
- Li L, Zhang Z. Isolation and characterization of a virulent bacteriophage SPW specific for Staphylococcus aureus isolated from bovine mastitis of lactating dairy cattle. Mol Biol Rep 2014, 41, 5829-5838. https://doi.org/10.1007/s11033-014-3457-2
- Nam HM, Lee AL, Jung SC, Kim MN, Jang GC, Wee SH, Lim SK. Antimicrobial susceptibility of Staphylococcus aureus and characterization of methicillin-resistant Staphylococcus aureus isolated from bovine mastitis in Korea. Foodborne Pathog Dis 2011, 8, 231-238. https://doi.org/10.1089/fpd.2010.0661
- Oliver SP, Headrick SI, Lewis MJ, Gillespie BE, Johnson DL, Almeida RA. Experimental intramammary infection with a strain of Escherichia coli isolated from a cow with persistent E. coli mastitis. Open J Vet Med 2012, 2, 186-190. https://doi.org/10.4236/ojvm.2012.24029
- Pozzo MD, Loreto ES, Santurio DF, Alves SH, Rossatto L, de Vargas AC, Viegas J, da Costa MM. Antibacterial activity of essential oil of cinnamon and trans-cinnamaldehyde against Staphylococcus spp. isolated from clinical mastitis of cattle and goats. Acta Sci Vet 2012, 40, 1080.
- Schukken YH, Wilson DJ, Welcome F, Garrison-Tikofsky L, Gonzalez RN. Monitoring udder health and milk quality using somatic cell counts. Vet Res 2003, 34, 579-596. https://doi.org/10.1051/vetres:2003028
- Sharma N, Jeong DK. Stem cell research: a novel boulevard towards improved bovine mastitis management. Int J Biol Sci 2013, 9, 818-829. https://doi.org/10.7150/ijbs.6901
- Sharma N, Singh NK, Bhadwal MS. Relationship of somatic cell count and mastitis: an overview. Asian-Australas J Anim Sci 2011, 24, 429-438. https://doi.org/10.5713/ajas.2011.10233
- Souza EL, Oliveira CEV, Stamford TLM, Conceicao ML, Gomes Neto NJ. Influence of carvacrol and thymol on the physiological attributes, enterotoxin production and surface characteristics of Staphylococcus aureus strains isolated from foods. Braz J Microbiol 2013, 44, 29-35. https://doi.org/10.1590/S1517-83822013005000001
- Taga I, Lan CQ, Altosaar I. Plant essential oils and mastitis disease: their potential inhibitory effects on proinflammatory cytokine production in response to bacteria related inflammation. Nat Prod Commun 2012, 7, 675-682.
- Teixeira B, Marques A, Ramos C, Serrano C, Matos O, Neng NR, Nogueira JMF, Saraiva JA, Nunes ML. Chemical composition and bioactivity of different oregano (Origanum vulgare) extracts and essential oil. J Sci Food Agric 2013, 93, 2707-2714. https://doi.org/10.1002/jsfa.6089
- Truchetti G, Bouchard E, Descoteaux L, Scholl D, Roy JP. Efficacy of extended intramammary ceftiofur therapy against mild to moderate clinical mastitis in Holstein dairy cows: a randomized clinical trial. Can J Vet Res 2014, 78, 31-37.
- Wu J, Hu S, Cao L. Therapeutic effect of nisin Z on subclinical mastitis in lactating cows. Antimicrob Agents Chemother 2007, 51, 3131-3135. https://doi.org/10.1128/AAC.00629-07
- Zhen YH, Jin LJ, Li XY, Guo J, Li Z, Zhang BJ, Fang R, Xu YP. Efficacy of specific egg yolk immunoglobulin (IgY) to bovine mastitis caused by Staphylococcus aureus. Vet Microbiol 2009, 133, 317-322. https://doi.org/10.1016/j.vetmic.2008.07.016
- Effects of intramammary infusion of sage (Salvia officinalis) essential oil on milk somatic cell count, milk composition parameters and selected hematology and serum biochemical parameters in Awassi sheep with subclinical mastitis vol.10, pp.8, 2017, https://doi.org/10.14202/vetworld.2017.895-900
Supported by : Daehan New Pharm Co. Ltd.