Effect of temperature on photosynthetic capacity and influence of harvesting time on quality of Salvia miltiorrhiza Bunge

온도에 따른 단삼의 광합성 특성 및 수확시기가 품질에 미치는 영향

  • Seo, Young-Jin (Bonghwa Medicinal Plant Research Station, GyeonBuk Agricultural Technology Administration) ;
  • Kim, Jong-Su (Bonghwa Medicinal Plant Research Station, GyeonBuk Agricultural Technology Administration) ;
  • Kim, Sun-Hwa (KMF Co., Ltd.) ;
  • Kim, Mi-Yeon (KMF Co., Ltd.) ;
  • Jeong, Yong-Jin (KMF Co., Ltd.) ;
  • Seong, Gi-Un (Department of Food Science and Technology, Kyungpook National University) ;
  • Chung, Shin-Kyo (Department of Food Science and Technology, Kyungpook National University)
  • Received : 2015.09.22
  • Accepted : 2015.11.04
  • Published : 2015.12.30


Salvia miltiorrhiza has been used for treating heart and liver disease. In the present study, the influences of temperature on photosynthetical capacity of S. miltiorrhiza under controlled cultivation environment using growth chamber were investigated because of providing information about growth and secondary metabolite synthesis. And effect of harvesting time on growth properties and constituents such as salvianolic acid B, cryptotanshinone, tanshinone I, tanshinone IIA were evaluated. Maximum photosynthesis rate ($5.102{\mu}mol\;CO2/m2/s$) and net apparent quantum yield ($0.147{\mu}mol\;CO2/m2/s$), stomatal conductance (0.035 mmol/m2/s) and water use efficiency ($7.108{\mu}mol\;CO2/mmol\;H2O$) was highest at $20^{\circ}C$. Results of chlorophyll fluorescence showed that elevated temperature had contributed to reduce a quantum yield and electron flux in photosystem. This result demonstrated that favorable temperature condition was determined at $20^{\circ}C$. Contents of salvianolic acid B, cryptotanshinone, tanshinone I and tanshinone IIA was highest in root sample harvested at 20 March, whereas growth and yield of S. miltiorrhiza had no significant differences with harvesting time. Therefore, this study shows that temperature play an important role in photosynthetic activity and harvesting time have influence upon accumulation of constituents in root of S. miltiorrhiza.


Grant : 국내산 단삼의 고랭지 재배기술 및 유효성분 강화 가공기반 구축

Supported by : 농림수산식품기술평가원


  1. Mok JS, Kim YM, Kim SH, Chang DS (1995) Antimicrobial properties of the ethanol extract from Salvia miltiorrhiza. J Food Hygie Safety, 10, 23-28
  2. Kim JK (1989) Illustrated natural drugs encyclopedia. Namsandang Publishers, Seoul, Korea, p 160
  3. Lee YJ, Lee SY (1992) Pharmacognocy. Dongmyeungsa, Seoul, Korea, p 131-137
  4. Fang ZY, Lin R, Yuan BX, Yang GD, Liu Y, Zhang H (2008) Tanshinone IIA down-regulates the CD40 expression and decreases MMp-2 activity on atherosclerosis induced by high fatty diet in rabbit. J Ethnopharma, 115, 217-222
  5. Ji W, Gong BQ (2008) Hypolipidemic activity and mechanism of purified herbal extract of Salvia miltiorrhiza in hyperlipidemic rats. J Ethnopharma, 119, 291-298
  6. Zhang F, Zheng W, Pi R, Mei Z, Bao Y, Gao J, Tang W, Chen S, Liu P (2009) Cryptotanshinone protects primary rat cortical neurons from glutamate induced neurotoxicity via the activation of the phosphatidyinositol 3-kinase/Akt signaling pathway. Experi Brain Res, 193, 109-118
  7. Kim JY, Kang HS, Choi JS, Yokozawa T, Chung HY (2008) Antioxidant potential of dimethyl lithospermate isolated from Salvia miltiorrhiza (red sage) against peroxynitrile. J Med Food, 11, 21-28
  8. Yin HQ, Choi YJ, Kim YC, Sohn DH, Ryu SY, Lee BH (2009) Salvia miltiorrhiza Bunge and its active component cryptotanshinone protects primary cultured rat hepatocytes form acute ethanol induced cytotoxicity and fatty infiltration. Food Chem Toxicol, 47, 98-103
  9. Choi HY, Han YS (2003) Isolation and identification of antimicrobial compound from Dansam (Salvia miltiorrhiza Bunge). J Korean Soc Food Sci Nutr, 32, 22-28
  10. Han WS (2004) Isolation of antimicrobial compounds from Salvia miltiorrhiza Bunge. Korean J Med Crop Sci, 12, 179-182
  11. Yang SA, Im NK, Lee IS (2007) Effect of methanolic extract from Salvia miltiorrhiza Bunge on in vitro antithrombotic and antioxidative activities. Korean J Food Sci Technol, 39, 83-87
  12. Yang BJ, Huang XL, Jhou QR (1984) The structures of four minor diterpenquinones przewaquinones C, D, E and F from the root of Salvia przewalskii Maxim var. mandarinorum (Diels) Stib. Acta Pharma Sinica, 19, 294-298
  13. Cheng GC, Lee JY, Kim DC, Suh SO, Hwang WI (2000) Inhibitory effect of Salvia miltiorrhiza extract on growth of some cancer cells. J Korean Soc Food Nutr, 29, 726-731
  14. Kim OH, Chung SY, Park MK, Rheu HM, Yang JS (1999) Anticancer activity of natural products including Salvia miltiorrhiza. J Appl Pharmacol, 7, 29-34
  15. Fugh-Berman A (2000) Herbs and dietary supplements in the prevention and treatments of cardiovescular disease. Preven Cardiol, 3, 24-32
  16. Wang XH, Morris-Natschke SL, Lee KH (2007) New developments in the chemistry and biology of bioactive constituents of Tanshen. Med Res Rev, 27, 133-148
  17. Zhou L (2005) Danshen : an overview of its chemistry, pharmacology, pharmacokinetics and clinical use. J Clin Pharmacol, 45, 1345-1359
  18. Laule O, Furholz A, Chang HS, Zhu T, Wang X, Heifetz PB, Gruissem W, Lange BM (2003) Crosstalk between cytosolic and plastidal pathways of isoprenoid biosynthesis in Arabidopsis thaliana. Proc Nat Acad Sci USA, 100, 6866-6871
  19. Lichtenhaler HK (2000) Non-mevalonate isoprenoid biosynthesis : enzyme, genes and inhibitors. Biochem Soc Trans, 28, 785-789
  20. Petersen M (1997) Cytochrome P450-dependent hydroxylation in the biosynthesis of resemarinic acid in Coleus. Phytochem, 45, 1165-1172
  21. Petersen M, Simmonds MSJ (2003) Rosemarinic acid. Phytochem, 62, 121-125
  22. Petersen M, Abdullah Y, Benner J, Eberle D, Gehlen K, Hucherig S, Janiak V, Kim KH, Sander M, Weitzel C, Wolters S (2009) Evolution of rosemarinic acid biosynthesis. Phytochem, 70, 15-16
  23. Seong NS, Lee SW, Kim KS, Lee ST (1993) Environmental variation of decursine content in Angelica gigas. Korean J Med Crop Sci, 1, 60-65
  24. Kim YK, Ahn YS, An TJ, Yeo JH, Park CB, Park HK (2009) Effect of yield and decursin content according to the accumulative temperature and seedling size in cultivation area of Angelica gigas Nakai. Korean J Med Crop Sci, 17, 458-463
  25. Jin YX, Cho YS, Choi YM (2014) Nutritional quality of Peucedanum japonicum Thunb. leaves in relation to ripening time, growing condition and blanching. Korean J Food Preserv, 21, 784-789
  26. Kim YK, Lee SH, Han SH, Kang YG, Ahn YS, Park CB (2010) Characteristics of flowering and seed setting of Salvia miltiorrhiza Bunge. Paper presented at 2010 Annual Meeting of Korean J Med Crop Sci, October 12, Jecheon, Korea
  27. Kim YK, Lee SH, Han SH, Kang YG, Lee YS, Ahn YS, Park CB (2011) Effect of fertilizer amount on growth and root yield in Salvia miltiorrhiza Bunge. Paper presented at 2011 Annual Meeting of Korean J Med Crop Sci, April 28, Jeju, Korea
  28. Lee SH, Kim YK, Han SH, Lee YS, Han NH, Kim SS, Song BH, Park CB (2012) Effect of biodegradable mulching on the weed management and growth characteristics in Salvia miltiorrhiza Bunge. Paper presented at 2012 Annual Meeting of Korean J Med Crop Sci, May 3, Cheongju, Korea
  29. Kim PG, Lee E.J (2001) Ecophysiology of photosynthesis : effect of light intensity and intercellular $CO_2$ pressure on photosynthesis. Korean J Agri Forest Meteor, 3, 126-133
  30. Kim PG, Lee KY, Kim SH, Han SS (1999) Foliar characteristics and photosynthetic efficiency of three species of Schizandracea trees distributed in Korea. Korean J Agri Forest Meteor, 1, 90-96
  31. Strasser RJ, Srivastava A, Tsimilli-Michael M (2000) The fluorescence transient as a tool to characterize and screen photosynthic sample. In: Probing photosynthesis : mechanism, regulation and adaptation, Yunus M et al., (Editor) Taylor and Francis, NY, USA, p 445-483
  32. Evans JR (1987) The dependence of quantum yield on wavelength and growth irradiance. Austrailian J Plant Physiol, 14, 445-483
  33. Kang SB, Jang HI, Lee IB, Park JM, Moon DK (2007) Changes in photosynthesis and chlorophyll fluoroescence of 'Cambell Early' and 'Kyoho' grapevine cultivars under long-term waterlogging condition. Korean J Hort Sci Technol, 25, 1-8
  34. Oh SJ, Zhin KL, Koh SC (2009) Characterization of Chl a fluorescence of hydrophytes under cadmium stress. Environ Sci, 18, 1361-1368