Study of antioxidant activities of extracts from Metaplexis japonica leaf and stem

박주가리(Metaplexis japonica Makino) 잎과 줄기 추출물의 항산화 효과에 관한 연구

  • Hong, Ju-Yeon (Faculty of Herbal Food Cuisine and Nutrition, Daegu Haany University) ;
  • Shin, Seung-Ryeul (Faculty of Herbal Food Cuisine and Nutrition, Daegu Haany University)
  • 홍주연 (대구한의대학교 한방식품조리영양학부) ;
  • 신승렬 (대구한의대학교 한방식품조리영양학부)
  • Received : 2015.11.03
  • Accepted : 2015.11.25
  • Published : 2015.12.30


The purpose of this study was to evaluate the antioxidant activity of Metaplexis japonica by using hot-water and ethanol extracts of its leaf and stem. Yields of hot-water and ethanol extracts of M. japonica leaf were high at 6.89 and 6.23%, respectively. The polyphenol and flavonoid contents in ethanol extracts of M. japonica leaf (ALEE) were high (86.96 and 60.73 mg/g, respectively). The electron-donating ability of all M. japonica extracts increased with an increase in extract concentration, with the highest electron-donating ability of 36.20~68.19% shown by hot-water extracts of M. japonica leaf (ALWE). The superoxide dismutase (SOD)-like activities of ALWE and ALEE increased with an increase in extract concentration. The nitrite-scavenging ability of the extracts was the highest at pH 1.2 and that of ALWE was higher than that of ALEE. The reducing power of $62.5{\mu}g/mL$ ALEE was 0.09 and that of $1,000{\mu}g/mL$ was 0.44. The inhibitory effect of an ethanol extract of M. japonica stem (ASEE) on tyrosinase was 13.81% at a concentration of $62.5{\mu}g/mL$ and that of $1,000{\mu}g/mL$ ALEE was 57.04%.


  1. MeCord JM (1987) Oxygen derived radicals : a link between repercussion injury and inflammation. Fed Proc, 46, 2402-2406
  2. Imalay IA, Lim S (1989) DNA damage and oxygen radical toxicity. Science, 240, 1302-1309
  3. Cho JS (1997) Food Material Science. Moon Woon Dang, Seoul, Korea, p 267
  4. Lee HS (1997) Dietary fiber intake of Korea. J Korean Soc Food Sci Nutr, 25, 540-548
  5. Choi BD (2010) Analysis of the contents and physiological activities of Calystegia japonica leaf extracts. Korean J Food Sci Technol, 42, 250-255
  6. Vidlea LA, Fermandez V (1998) Biochemical aspects of cellular oxidative stress. Arch Biol Med Exp, 21, 85-92
  7. Halliwell B, Aruoma OJ (1991) DNA damage by oxygen-derived species. FEBS Lett, 2811, 9-19
  8. Jennings PE, Barnett AH (1988) New approaches to the pathogenesis and treatment of diabetic microangicpathy. Diabetic Med, 5, 111-117
  9. Fridovich I (1989) Superoxide dismutase. An adaption to a paramagnetic gas. J Biol Chem, 264, 7761-7764
  10. Branen AL (1991) Toxicological and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene. J Am Oil Chem Soc, 52, 59-63
  11. Barlow SM (1990) Toxicological aspects of antioxidants used as food additives. In: Food Antioxidant, Hudson BJF (Editor). Elsevier, Amsterdam, p 253-307
  12. Eliott JG (1999) Application of antioxidant vitamins in foods and beverages. Food Tech, 53, 46-48
  13. Yamamoto K, Niki E (1988) Proxidant effect of $\alpha$-tocopherol. Biochem. Biophys Acta, 958, 19-23
  14. Nam SH, Kang MY (2000) Screening of antioxidative activity of hot-water extracts from medicinal plants. J Korean Soc Agric Chem Biotecnol, 43, 141-147
  15. Kim SM, Cho YS, Sung SK (2001) The antioxidant ability and nitrite scavenging ability of plant extracts. Korean J Food Sci Technol, 33, 626-632
  16. Yang SA, Im NK, Lee IS (2007) Effects of methanolic extract from Salvia miltiorhiza Bunge on in vitro antihrombotic and antioxdiative activities. Korean J Food Sci Technol, 39, 83-87
  17. Yoon GA (2006) Effect of garlic supplement and exercise on plasma lipid and antioxidative enzyme system in rats. Korean Food Nutr Sci, 39, 3-10
  18. Han SH, Woo NY, Lee SD, Kang MH (2006) Antioxidative and antibacterial activities of endemic plants extracts in Korea. Korean J Medicinal Crop Sci, 14, 49-55
  19. Kim HY, Park YK, Kim TS, Kang MH (2006) The effect of green vegetable drink supplementation on cellular DNA damage and antioxidant status of Korean smokers. Korean Food Nutr Sci, 39, 18-27
  20. Branen AL (1975) Toxicology and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene. J Am Oil Chem Soc, 52, 59-63
  21. Jorge M, Ricardo DS, Jacques R, Vernonique C, Anni C, Michel M (1991) Procyanidin dimers and trimers from grape seeds. Phytochem, 30, 1259-1264
  22. Lee SY, Kim JS, Kang SS (2012) Flavonol glycosides from the aerial parts of Metaplexis japonica. Kor J Pharmacogn, 43, 206-212
  23. Kim MJ, Kim IJ, Choi SY, Han DH, Kim YH, Lim SC, Kim TJ, Nam SY, Song BH, Oh BU, Park CG (2014) Comparison of Cynanchum wilfordii, C. auriculatum, Metaplexis japonica and Polygonum multiflorum by morphological characters. Korean J Medicinal Cro Sci, 22, 113-120
  24. Singleton VL, Rossi A (1965) Colorimetry of total phenolics with phosphotungstic acid reagents. Am J Enol Viticult, 16, 144-158
  25. Moreno VL, Isla MI, Sampietro AR, Vattuone MA (2000) Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol, 71, 109-114
  26. Blois ML (1958) Antioxidant determination by the use of a stable free radical. Nature, 181, 1199-1224
  27. Marklund S, Marklund G (1974) Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem, 47, 469-474
  28. Kato H, Lee IE, Chuyen NV, Kim SB, Hayase F (1987) Inhibitory of nitrosamine formation by nondilyzable melanoidins. Agric Biol Chem, 51, 1333-1338
  29. Stirpe F, Corte ED (1969) The regulation of rat liver xanthine oxidase. J Biol Chem, 244, 3855-3861
  30. Yagi A, Kanbara T, Morinobu N (1987) The effect of tyrosinase inhibition by aloe extract. Planta Medica, 53, 515-517
  31. Wong JY, Chye FY (2009) Antioxidant properties of selected tropical wild edible mushrooms. J Food Comp Anal, 22, 269-277
  32. Park WP (2014) Quality characteristics of noodles added with Houttuynia cordata Thunb powder. Korean J Food Preserv, 21, 34-39
  33. Moon JS, Kim SJ, Park YM, Hwang JS, Kim EH, Park JW, Park IB, Kim SW, Kang SG, Park YK, Jung ST (2004) Activities of antioxidation and alcohol dehydrogenase inhibition of methanol extracts from some medicinal herbs. Korean J Food Preserv, 11, 201-206
  34. Lee SO, Lee HJ, Yu MH, Im HG, Lee IS (2005) Total polyphenol contents and antioxidants activities of methanol extracts from vegetables produced in Ullung island. Korean J Food Sci Technol, 37, 233-240
  35. Isono R. Yomokazu T, Esumi K (2005) Preparation of $Au/TiO_2$ nano composities and their catalytic activity for DPPH radical scavenging reaction. J Colloids Interf Sci, 288, 177-183
  36. Lim JD, Yu CY, Kim MJ, Yun SJ, Lee SJ, Kim NY, Chung IM (2004) Comparison of SOD activity and phenolic compound contents in various Korean medicinal plants. Korean J Medicinal Crop Sci, 12, 191-202
  37. Kytopoulos SA (1987) Ascorbic acid and formation of N-nitroso compounds, possible role of ascorbic acid in cancer prevention. Am J Clin Nutr, 45, 1344-1350
  38. Takashi Y, Yamamoto M, Tamura A (1978) Studies on the formation of nitrosamines. The effects of some polyphenols on nitrosation of diethylamine. J Food Hyg Soc, 19, 224-229

Cited by

  1. Metajapogenins A–C, Pregnane Steroids from Shells of Metaplexis japonica vol.22, pp.4, 2017,