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Effect of submerged culture of Ceriporia lacerata mycelium on GLUT4 protein in db/db mouse

db/db 마우스에서 Ceriporia lacerata 균사체 배양액이 GLUT4 발현에 미치는 영향

  • 신은지 ((주)퓨젠바이오농업회사법인) ;
  • 김지은 (계명대학교 식품가공학과) ;
  • 김지혜 (계명대학교 의과대학) ;
  • 박용만 ((주)퓨젠바이오농업회사법인) ;
  • 윤성균 ((주)퓨젠바이오농업회사법인) ;
  • 장병철 (계명대학교 의과대학) ;
  • 이삼빈 (계명대학교 식품가공학과) ;
  • 김병천 ((주)월드바이오텍)
  • Received : 2015.10.07
  • Accepted : 2015.11.23
  • Published : 2015.12.30

Abstract

In this study, we evaluated the antidiabetic effect of a submerged culture of Ceriporia lacerata mycelium (CL01) on hematological indices, as well as protein and mRNA expression of the insulin-signaling pathway, in db/db mice. After CL01 was administrated for 4 weeks, blood glucose levels decreased consistently, and plasma insulin and c-peptide levels each decreased by roughly 55.8%, 40% of those in the negative control (p<0.05). With regard to HOMA-IR, an insulin resistance index, insulin resistance of the CL01-fed group improved over that of the negative control group by about 62% (p<0.05). In addition, we demonstrated that the protein expression levels of pIR, pAkt, pAMPK, and GLUT4 and the mRNA expression levels of Akt2, IRS1, and GLUT4 in the muscle cells of db/db mice increased in the CL01-fed group compared to the corresponding levels in the control group. These results demonstrate that CL01 affects glucose metabolism, upregulates protein and gene expression in the insulin-signaling pathway, and decreases blood glucose levels effectively by improving insulin sensitivity. More than 90% of those who suffer from type 2 diabetes are more likely to suffer from hyperinsulinemia, hypertension, obesity, and other comorbidities because of insulin resistance. Therefore, it is possible that CL01 intake could be used as a fundamental treatment for type 2 diabetes by lowering insulin resistance, and these results may prove be useful as basic evidence for further research into the mechanisms of a cure for type 2 diabetes.

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