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Allergic risk assessment of genetically modified cucumber mosaic virus resistant pepper

유전자변형 바이러스 저항성 고추의 알레르기 안전성

  • Son, Dae-Yeul (Department of Food and Biotechnology, Daegu Haany University)
  • 손대열 (대구한의대학교 바이오산업융합학부)
  • Received : 2015.09.24
  • Accepted : 2015.11.04
  • Published : 2015.12.30

Abstract

Genetically modified (GM) pepper H15 containing the gene for cucumber mosaic virus (CMV) coat protein (CP) and its control line non-GM pepper P2377 were investigated for their allergic risk. Amino acid sequence of the inserted gene product CMV-CP was compared with those of known allergens. No known allergen had greater than 35% amino acid sequence homology over an 80 amino acid window or more than 8 consecutive identical amino acids. Protein patterns of GM and non-GM pepper extracts were evaluated by SDS-PAGE, which showed similar distribution of protein bands for both GM and non-GM pepper. Antigen-antibody reactions were compared between GM and its non-transgenic parental control. ELISA and immunoblot analysis of sera from allergic patients showed some IgE reactivity; however, no differences were observed between GM pepper H15 and P2377. We therefore conclude that CMV-CP is less likely to be an allergen; the protein composition and allergenicity of the GM pepper H15 is not different from that of P2377 and safe as a commercial host.

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