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Effect of Lime Sulfur on Changes of Fungal Diversity in Pear Fallen Leaves

석회유황합제가 배나무 낙엽의 진균 다양성 변화에 미치는 영향

Min, Kwang-Hyun;Song, Jang Hoon;Cho, Baik Ho;Yang, Kwang-Yeol
민광현;송장훈;조백호;양광열

  • Received : 2015.11.23
  • Accepted : 2015.12.14
  • Published : 2015.12.31

Abstract

This study was conducted to examine changes in the fungal community on fallen leaves of pear by treatment with lime sulfur. Although the lime sulfur could reduce the primary inoculum of several pathogens on spring season, the effect of lime sulfur has not been well determined scientifically. Fallen leaves infected by pear diseases in pear orchards in Naju were collected and treated with lime sulfur or water as a control. To determine the fungal diversity from each treatment, rDNA internal transcribed spacer (ITS) regions were analyzed after extraction of fungal genomic DNA from lime sulfur-treated or water-treated fallen leaves, respectively. The most common fungal species were Ascomycota and Basidiomycota in both treated leaves. However, the population dynamics of several fungal species including Alternari sp., Cladosporium sp., and Phomopsis sp., which are known as pear pathogens for skin sooty dapple disease, were quite different from each treated leaves. These results indicated that lime sulfur treatment led to changes of fungal communities on pear fallen leaves and could be applicable as a dormant spray.

Keywords

Fallen leaves;Fungal diversity;Lime sulfur;Pear

References

  1. Yoon DH, Park HJ, Nam KW. Control effect of environmental-friendly organic materials against major pear diseases. Korean J Pestic Sci 2010;14:401-6.
  2. Agrios GN. Plant pathology. 5th ed. Boston: Elsevier Academic Press; 2005.
  3. McCallan, SE. The nature of the fungicidal action of copper and sulfur. Bot Rev 1949;15:629-43. https://doi.org/10.1007/BF02861716
  4. Park YS. Studies on the skin sooty dapple disease of oriental pear in Korea [dissertation]. Gwangju: Chonnam National University; 2004.
  5. Park YS, Kim KC, Lee JH, Kim IS, Choi YS, Cho SM, Kim YC. Etiology and chemical control of skin sooty dapple disease of Asian Pear. Korean J Pestic Sci 2008;12:375-81.
  6. Yoon JT, Kim JS, Kang CS. Effective chemical control system of apple scab caused by Venturia inaequlis. Plant Pathol J 1993;9:180-4.
  7. Willamson SM, Sutton TB. Sooty blotch and flyspeck of apple: etiology, biology, and control. Plant Dis 2000;84:714-24. https://doi.org/10.1094/PDIS.2000.84.7.714
  8. Holb IJ, De Jong PF, Heijne B. Efficacy and phytotoxicity of lime sulphur in organic apple production. Ann Appl Biol 2003;142:225-33. https://doi.org/10.1111/j.1744-7348.2003.tb00245.x
  9. Montag J, Schreiber L, Schonherr J. An in vitro study on the postinfection activities of hydrated lime and lime sulphur against apple scab (Venturia inaequalis). J Phytopathol 2005;153:485-91. https://doi.org/10.1111/j.1439-0434.2005.01007.x
  10. Jamar L, Lefrancq B, Fassotte C, Lateur M. A during-infection spray strategy using sulphur compounds, copper, silicon and a new formulation of potassium bicarbonate for primary scab control in organic apple production. Eur J Plant Pathol 2008;122:481-93. https://doi.org/10.1007/s10658-008-9315-0
  11. Kang HW. Genetic diversity analysis of fungal species by universal rice primer (URP)-PCR. Kor J Mycol 2012;40:78-85. https://doi.org/10.4489/KJM.2012.40.2.78
  12. White TJ, Bruns T, Lee S, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, editors. PCR protocols: a guide to methods and applications. San Diego: Academic Press; 1990. p. 315-22.
  13. Le Cam B, Devaux M, Parisi L. Specific polymerase chain reaction identification of Venturia nashicola using internally transcribed spacer region in the ribosomal DNA. Phytopathology 2001;91:900-4. https://doi.org/10.1094/PHYTO.2001.91.9.900
  14. Moon HY. Ecophysiological characteristics of Venturia nashicola Tanaka & Yamamoto causing pear scab and screening of biocontrol agent against the causal fungus. [MS Thesis]. Gwangju: Chonnam National University; 2009.
  15. Nam KW, Oh SY, Yoon DH. Pear skin stain caused by Mycosphaerella graminicola on Niitaka pear (Pyrus pyrifolia Nakai). Plant Pathol J 2014;30:229-35. https://doi.org/10.5423/PPJ.OA.04.2014.0030