Initiation of embryogenic callus from mature zygotic embryos in Japanese larch (Larix kaempferi)

  • Kim, Yong-Wook
  • Received : 2015.09.11
  • Accepted : 2015.09.23
  • Published : 2015.09.30


Embryogenic callus (EC) was created from mature embryos of Larix kaempferi. With the mature embryos, keeping the culture in dark conditions throughout the experiment (38.2%) seemed to give better results than exposing them to 16 h light ($25{\mu}Em^{-2}s^{-1}$) for the first week (21.9%). EC was obtained most frequently from Quoirin and Lepoivre (LP) mediums with 1.0 mg/L 4-amino-3,5,6-trichloropicolinic acid (Picloram), plus 1.0 mg/L benzyladenine (BA) (62.8%) or Litvay's medium (LM) containing 1.0 mg/L p-chlorophenoxyacetic acid (pCPA) plus 1.0 mg/L BA (62.8%) treatment. In both cases, best results were obtained when zygotic embryos were cultured in darkness. As for the effective sucrose concentration on initiation of EC, 29.2 mM sucrose (38.6%) gave the best results.


Embryogenic callus;Japanese larch;Somatic embryos;Zygotic mature embryos


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Grant : 소나무 및 낙엽송 체세포배 클론묘 생산효율 증진 기술개발