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희귀 및 멸종위기 식물 미선나무(Abeliophyllum distichum Nakai)의 절편위치 및 치상방법에 따른 기내증식 및 미세삽목

이나념;김지아;김용욱;최용의;문흥규
Lee, Na Nyum;Kim, Ji-Ah;Kim, Yong-Wook;Choi, Yong Eui;Moon, Heung Kyu

  • 투고 : 2015.08.10
  • 심사 : 2015.09.23
  • 발행 : 2015.09.30

초록

본 실험은 희귀 및 멸종위기 식물인 미선나무의 효과적인 증식을 위하여 줄기 증식, 생장 및 발근에 미치는 절편의 위치 효과 및 치상 방법의 효과를 구명하기 위해 실시되었다. 줄기유도는 BA를 처리한 액아절편 배양이 효과적이었으며 절편 당 2.4개의 줄기가 유도되었다. 정아 및 액아절편을 BA 1.0 mg/L를 전처리하여 수평과 수직으로 치상하였을 때 줄기유도는 액아 절편의 수직치상으로 절편 당 2.5개로 가장 좋았다. 반면 줄기의 생장, 발근 및 뿌리 발달은 정아절편의 수직치상에서 양호하였고 BA의 전처리 시간이 길어질수록 발근이 억제되는 경향을 보였다. 기내증식된 줄기의 정아 및 액아를 절편으로 기외에서 미세삽목(micro-cutting)을 실시하여정아절편에서 100% 발근되었고, 뿌리수는 개체 당 평균 6.2개로 가장 양호하였다. 미세삽목묘는 온실에서 95% 이상 순화되었다. 이상의 결과로 볼 때 미선나무의 기내 증식은 액아를 절편으로 BA 처리 후 수직 치상하는 방법이 효과적이며, 줄기의 생장 및 발근은 정아를 절편으로 수직치상 하는 것이 효과적으로 나타났다. 또한 정아를 절편으로 미세삽목을 통해 효과적인 발근 유도 및 순화가 가능하여 미선나무의 실용적인 묘목생산이 가능함을 보여주었다.

키워드

Abeliophyllum distichum Nakai;Apical or axillary bud explants;Cultural method;Micropropagation

참고문헌

  1. Azamal H, Mohinder P (2007) Effect of branch position and auxin treatment on clonal propagation of Tectona grandis Linn. f. New Forest 34:223-233 https://doi.org/10.1007/s11056-007-9050-y
  2. Bhardwaj DR, Mishra VK (2005) Vegetative propagation of Ulmus villosa: Effects of plant growth regulators, collection time, type of donor and position of shoot on adventitious root formation in stem cuttings. New Forest 29:105-116 https://doi.org/10.1007/s11056-005-0240-1
  3. Chen UC, Hsia CN, Yeh MS, Agrawal DC, Rsay HS (2006) In vitro micropropagation and ex vitro acclimation of Bulpleurum kaoi- an endangered medicinal plant native to Taiwan. In Vitro Cell Dev Biol Plant 42:128-133 https://doi.org/10.1079/IVP2005744
  4. Debergh PC (1988) Micropropagation of woody species-state of the art on in vitro aspects. Acta Hortic 227:287-295
  5. Duong Tan Nhut, Bui Van Le, Seiichi Fukai, Michio Tanaka, K. Tran Thanh Van (2001) Effects of activated charcoal, explant size, explant position and sucrose concentration on plant and shoot regeneration of Lilium longiflorum via young stem culture. Plant Growth Regul 33:59-65 https://doi.org/10.1023/A:1010701024963
  6. Duong Tan Nhut, Truong Thi Thuy An, Nguyen Thi Dieu Huong, Nguyen Trinh Don, Nguyen Thanh Hai, Nguyen Quoc Thien, Nguyen Hong Vu (2007) Effect of genotype, explant size, position, and culture medium on shoot generation of Gerbera jamesonii by receptacle transverse thin cell layer culture. Sci Hortic-Amaterdam 111:146-151 https://doi.org/10.1016/j.scienta.2006.10.008
  7. El-Morsy AA, Millet B (1996) Rhythmic growth and optimization of micropropagation: The effect of excision time and position of axillary buds on in vitro culture of Citrus aurantium L. Ann Bot-London 78:197-202 https://doi.org/10.1006/anbo.1996.0113
  8. Faisal M, Ahmad N, Anis M (2007) An efficient micropropagation system for Tylophora indica. an endangered, medicinally important plant. Plant Biotech Rep 1:155-161 https://doi.org/10.1007/s11816-007-0025-4
  9. Gomes GAC, Paiva R, Paiva PDO, Santiago EJAD (2003) Plant regeneration from callus cultures of Maclura tinctoria, an endangered woody species. In Vitro Cell Dev Biol Plant 39:293-295 https://doi.org/10.1079/IVP2002401
  10. Han MS, Park SY, Moon HK, Kang YJ (2010) Micropropagation of a rare tree species, Empetrum nigrum var. japonicum K. Koch via axillary bud culture. J Kor Forest Soci 99(4): 568-572
  11. Hsueh-Shih Lin, Marjo J, De Jeu, Evert Jacobsen (1998) Formation of shoots from leaf axils of Alstroemeria: The effect of the position on the stem. Plant Cell Tiss Org 52:165-169 https://doi.org/10.1023/A:1006063105940
  12. Husen A (2004) Clonal propagation of Dalbergia sissoo Roxb. By softwood nodal cuttings: Effects of genotypes, application of IBA and position of cuttings on shoots. Silvae Genet 53:50-55 https://doi.org/10.1515/sg-2004-0009
  13. Kim CS, Kim ZS (2012) Effects of cutting time, auxin treatment and cutting position on rooting of the green-wood cuttings and growth characteristics of transplanted cuttings in the adult Prunus yedoensis. Kor J Horticul Sci Tech 30(2):129-136 https://doi.org/10.7235/hort.2012.11041
  14. Lardet, Enjalric F, Carron MP (1990) Hevea brasiliensis (Mull. Arg.) apex cultures. Influence of the morphogenetical stage and explant age. Comptes Rendus de l'AcadeUmie des Sciences Serie III-Sciences de la Vie 310(5):195-202
  15. Leakey RRB (1983) Stock Plant factors affecting root initiation in cuttings of Triplochiton Scleroxylon K. Schum an indigenous hardwood of West Africa. J Hortic Sci Biotech 58:227-290
  16. Lee NN, Choi YE, Moon HK (2014) Effect of LEDs on shoot multiplication and rooting of rare plant Abeliophyllum distichum Nakai. J Plant Biotechnol 41:94-99 https://doi.org/10.5010/JPB.2014.41.2.94
  17. Lee TB (1976) Illustrated flora of Korea. Hyangmun Press
  18. Lee TB (1990) Conservation of threatened plants in Korea. Bull Kwanak Arboretum. 3:190-196
  19. Maria Papafotiou, Aekaterini N, Martini (2009) Effect of position and orientation of leaflet explants with respect to plant growth regulators on micropropagation of Zamioculcas zamiifolia Engl. (ZZ). Sci Hortic-Amsterdam 120:115-120 https://doi.org/10.1016/j.scienta.2008.09.023
  20. Matin MA (1989) Carbon economy during rooting of cuttings of Nauclea diderrichii (De. Wild and Th. Dus.) Merill. M. Phil thesis, University of Edinburgh, United Kingdom
  21. Minal Mhatre, Bapat VA, Rao PS (1985) Regeneration of plants from the culture of leaves and axillary buds in mulberry (Morus indica L.). Plant Cell Rep 4:78-80 https://doi.org/10.1007/BF00269211
  22. Mitsukuri K, Mori G, Johkan M, ShimadaY, Mishiba K-I, Morikawa T, Masayuki Oda (2009) Effects of explant position and dark treatment on bud formation in floret culture of Ponerorchis graminifolia Rchb.f. Sci Hortic-Amsterdam 121:243-247 https://doi.org/10.1016/j.scienta.2009.01.033
  23. Moon HK, Kim YW (2008) In vitro propagation of a rare and endangered species, Echinosophora koreensis Nakai, by axillary bud culture. J Plant Biotechnol 35(3):229-234 https://doi.org/10.5010/JPB.2008.35.3.229
  24. Moon HK, Noh EW, Ha YM, Shim KK (2002) Micropropagation of Juvenile and mature tree of Corylolopsis coreana by axillary bud culture. J Plant Biotechnol 29(2):117-121 https://doi.org/10.5010/JPB.2002.29.2.117
  25. Moon HK, Suk GY, Kim SC (1997) Micropropagation of a rare species, Forsythia saxatilis N. through tissue culture. J Kor Forest Soci 86(4):430-434
  26. Moon HK, Suk GY, Kwon YJ, Son SH (1999) Micropropagation of a rare species, Abeliophyllum distichum Nakai, via axillary bud culture. Kor J plant tiss culture 26(2):133-136
  27. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. J Plant Physiol 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  28. Negash L (2002) Successful vegetative propagation techniques for the threatened African pencil cedar (Juniperus procera Hoechst. Ex Endl.). Forest Ecol Manag 161:53-64 https://doi.org/10.1016/S0378-1127(01)00501-1
  29. Ofori D, Newton AC, Leakey RRB, Grace J (1997) Vegetative propagation of Milicia excelsa by leafy stem cuttings: Effects of maturation, coppicing, cutting length and position on rooting ability. J Tropical Forest Sci 10(1):115-129
  30. Paek KY (1998) Commercial aspects and problems in micropropagation. The 12th Symposium on Plant Biotechnology. Korea-Japan Joint Symposium. Frontier Researches in Plant Biotechnol 3:152-156
  31. Park SY, Ahn JK, Lee WY (2003) High frequency shoot induction from root segments of Albizzia coreana. J Kor Forest Soci 92:626-631
  32. Pereira AMS, Moro JR, Cerdeira RMM, Franca SC (1995) Effect of phytoregulators and physiological characteristics of the explants on micropropagation of Maytenus ilicifolia. Plant Cell Tiss Org 42:295-297 https://doi.org/10.1007/BF00030003
  33. Poupard C, Chauviere M, Monteuuis O (1994) Rooting Acacia mangium cuttings: Effects of age, within-shoot position and auxin treatment. Silvae Genet 43(4):226-231
  34. Sanjaya, Muthan B, Rathore TS, Rai VR (2006) Micropropagation of an endangered Indian sandalwood (Santalum album L.). J Forest Res 11:203-209 https://doi.org/10.1007/s10310-006-0207-x
  35. Youn Y, Lee SK, Park JI (1992) In vitro propagation of a rare species-Berchemia berchemiaefolia. Res Rep For Gen Res Inst Korea 28:63-67
  36. Vendrame WA, Maguire I, Carvalho VS (2007) In vitro propagation and plantlet regeneration from Doritaenopsis purple gem 'Ching Hua' flower explants. HortScience 42: 1256-1258
  37. Viemont J-D (1984) Les bruyeres in vitro. IV. Influence de la croissance rythmique sur l'evolution des bourgeons axillaires de l'Erica x darleyensis. Annales des Sciences Naturelles. Botanique. Paris 6:237-244

피인용 문헌

  1. Effect of kinds of medium and plant growth regulators for plantlets regeneration by bud culture in Disporum sessile vol.44, pp.1, 2017, https://doi.org/10.5010/JPB.2015.42.3.228