배추 무사마귀병 마커 탐색을 위한 배추 뿌리 단백질체 분석

DOI QR코드

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정재윤;임용표;황철호
Jeung, Jae Yun;Lim, Yong Pyo;Hwang, Cheol Ho

  • 투고 : 2015.11.03
  • 심사 : 2015.12.17
  • 발행 : 2015.12.31

초록

Clubroot disease is one of the most wide-spread and devastating diseases in the cultivation of Chinese cabbage. To develop a protein marker for resistance to clubroot disease in Chinese cabbage, a comparative proteome analysis was performed between a sensitive line, 94SK, and a resistant line, CR Shinki DH. Three proteins of two fold or higher accumulation that are specific to each line were found 3 days after innoculation of the Plasmodiphora brassicae. They are glutamine synthetase, malate dehydrogenase/oxidoreductase and fructose-bisphosphate aldolase in the 94SK and actin, phosphoglycerate kinase, and Cu/Zn superoxide dismutase in the CR Shinki line. From the comparison of the synthesized proteins in the 94SK and the CR Shinki, CR Shinki was found to produce more ATP-binding protein for the ABC transporter while 94SK showed a higher level of pathogenesis-related protein 1 production. All of these proteomic variations may lead to the development of molecular markers to accelerate the breeding process.

키워드

Clubroot disease;Plasmodiphora brassicae;Proteome;Root

참고문헌

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