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저온저장 음나무 배발생 캘러스로부터 체세포배 유도와 식물체 재생

이나념;최용의;문흥규
Lee, Na Nyum;Choi, Yong Eui;Moon, Heung Kyu

  • 투고 : 2015.10.15
  • 심사 : 2015.12.21
  • 발행 : 2015.12.31

초록

본 연구는 약용 및 식용으로 유망한 음나무의 대량생산 및 생식질 보존을 위한 기초 자료를 제공하기 위해 배발생 조직을 재료로 저온저장 기간별 SE 유도 및 식물체 재생을 시험하였다. 배발생 캘러스는 저온저장 6개월까지 정상적인 체세포배 형성이 가능하였으나 유도 빈도는 저장 기간에 따라 감소하는 경향을 보였다. 배발생능이 가장 좋은 ZE 10 세포주은 체세포배 유도 빈도에 있어 저장 기간에 따른 감소폭이 비교적 적은 것으로 나타났다. 체세포배의 발아 및 식물체 재생은 캘러스의 저장기간에 따라 다소 감소하는 경향을 보였으나 발아율은 93% 이상, 식물체 재생율은 91% 이상으로 저온저장에 따른 큰 문제는 없었다. 조직검경 결과 저장 기간에 따라 세포의 갈변화 및 활력 저하가 나타났으며, 아세토카민과 에반스블루의 염색을 통해서도 확인되었다. 이상의 결과로 볼 때 음나무 배발샐 캘러스의 저온저장은 계대배양 없이 $4^{\circ}C$의 냉장고에서 6개월 까지 가능한 것으로 나타났으며, 저장 후 체세포배 유도효율을 위해 배발생능이 양호한 세포주의 저장이 필요한 것으로 관찰되었다.

키워드

Cold storage;Embryogenic callus;Somatic embryo germination;Kalopanax setemlobus;Plant conversion

참고문헌

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피인용 문헌

  1. Biological activities ofBrassica rapa(Turnip) callus extracts by plant cell culture technology vol.43, pp.2, 2016, https://doi.org/10.5010/JPB.2015.42.4.388

과제정보

연구 과제번호 : 유용활엽수 우수목 증식을 위한 줄기세포 유도 기술 개발