Efficient Micropropagation of Pear Germplasm Using Soot Tips and Nodal Explants

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Yi, JungYoon;Lee, GiAn;Chung, JongWook;Lee, YoungYi;Gwag, JaeGyun;Lee, SeokYoung

  • 투고 : 2015.08.17
  • 심사 : 2015.10.23
  • 발행 : 2015.12.31

초록

We micropropagated pear (Pyrus species) using shoot tips and nodal explants from three pear genotypes. The ability to establish shoot tip cultures, proliferate shoots, induce rooting, and acclimatize the resulting plantlets are all elements of in vitro micropropagation. Shoots were induced from shoot tips on Murashige and Skoog medium (MS) with five different plant growth regulator combinations. The highest shoot formation rates were achieved for the three genotypes using MS supplemented with 1.0 mg/L N6-benzyladenine (BA) and 0.1 mg/L gibberellic acid (GA3). The maximum shoot number and shoot length for the three cultivars were recorded with 2.0 mg/L BA and 0.2 mg/L indole-3-butyric acid (IBA) in multiplication medium using nodal explants produced from microshoots. Nodal explants with one or two axillary buds cultured for three weeks initiated roots on medium supplemented with various concentrations of 1-naphthaleneacetic acid (NAA) or/and IBA in half-strength MS medium for adventitious rooting. The highest rooting response was with the combination of 0.2 mg/L NAA and 0.2 mg/L IBA. A combination of NAA and IBA resulted in a significant increase in the rooting ratio over NAA or IBA alone. In this medium, the root formation rate according to ranged from 68.9% for the BaeYun No. 3 genotype to 51.8% for the Hwanggeum genotype. We also investigated the influence of the concentration the polyamine phloroglucinol in rooting medium. For all three genotypes, the highest rooting ratio, longest root length, and greatest root number were observed in the treatments with 75-150 mg/L phloroglucinol. Most rooted plants were acclimatized successfully.

키워드

Micropropagation;Pear;Germplasm;Shoot tip;Nodal explant

참고문헌

  1. Cheng, T.V. 1979. Micropropagation of clonal fruit tree root-stocks. Compact Fruit Trees 12:127-137.
  2. Chang, Y. and B.M. Reed. 2001. Preculture conditions influence cold hardiness and regrowth of Pyrus cordata shoot tips after cryopreservation. HortScience 36:1329-1333.
  3. Caboni, E., M.G. Tonelli, P. Lauri, S. D’Angeli and C. Damiano. 1999. In vitro shoot regeneration from leaves of wild pear. Plant Cell Tiss. Organ Cult. 59:1-7. https://doi.org/10.1023/A:1006351214343
  4. Bhojwani, S.S., K. Mullins and D. Cohen. 1984. In vitro propagation of Pyrus pyrifolia. Scientia Hort. 23:247-254. https://doi.org/10.1016/0304-4238(84)90068-2
  5. Bell, R.L. and B.M. Reed. 2002. In vitro tissue culture of pear: Advances in techniques for micropropagation and germplasm preservation. Proc. 8th IS on pear. L. Corelli-Grappadelli et al. (eds.), Acta Hort. 596. ISHS. pp. 412-418.
  6. Anirudh, T. and J.S. Kanwar. 2008b. Micropropagation of 'wild pear' Pyrus pyrifolia (Burm F.) Nakai II. Induction of rooting. Not. Bot. Hort. Agrobot. Cluj. 36(2):104-111.
  7. Anirudh, T. and J.S. Kanwar. 2008a. Micropropagation of 'wild pear' Pyrus pyrifolia (Burm F.) Nakai I. Explant establishment and shoot multiplication. Not. Bot. Hort. Agrobot. Cluj. 36(1):103-108.
  8. Ancora, G., M.L. Belli-Donini and L. Cozzo. 1981. Globe artichoke plants obtained from shoot apices through rapid in vitro micropropagation. Sci. Hort. 14:207-213. https://doi.org/10.1016/0304-4238(81)90014-5
  9. Lundergan, C.A. and J. Janick. 1980. Regulation of apple shoot proliferation and growth in vitro. Hort. Res. 20: 19-24.
  10. Lane, D.W. 1979. Regeneration of pear plants from shoot meristem-tips. Plant Sci. Lett. 16:337-342. https://doi.org/10.1016/0304-4211(79)90046-4
  11. Kang, C.H., B.S. Han, S.G. Han, S.H. Kwon and Y.J. Song. 2011. Selection of suitable varieties of carnation (Dianthus caryophyllus L.) and optimization of culture conditions for efficient tissue culture. Korean J. Plant Res. 24:121-129 https://doi.org/10.7732/kjpr.2011.24.2.121
  12. James, D.J. and I.J. Thurbon. 1981. Phenolic compounds and other factors controlling rhizogenesis in vitro in the apple rootstocks M9 and M26. Zeitschrift für Pflanzenphysiologie. 105:11-20. https://doi.org/10.1016/S0044-328X(81)80003-7
  13. James, D.J. and I.J. Thurbon. 1979. Rapid in vitro rooting of the apple roostock M9. J. of Hort. Sci. 54:309-311.
  14. Hutchinson, J.F. and R.H. Zimmerman. 1989. Tissue culture of temperate fruit and nut trees. Hort. Rev. 9:273-349.
  15. Hu, C.Y. and P.J. Wang. 1983. Meristem, shoot-tip and bud culture: In Evans, D.A., W.R. Sharp, P.V. Ammirato and Y. Yamada (eds.), Handbook of Plant Cell Culture, Macmillan, New York, USA. pp. 177-277.
  16. Dwivedi, S.K. and L.D. Bris. 1999. In vitro propagation of low-chill pear cv. Gola. Indian J. Hort. 56:189-193.
  17. Condello, E., M.A. Palombi, M.G. Tonelli, C. Damiano and E. Caboni. 2009. Genetic stability of wild pear (Pyrus pyraster, Burgsd) after cryopreservation by encapsulation dehydration. Agricultural and Food Science 18:136-143. https://doi.org/10.2137/145960609789267533
  18. Chevreau, E., B. Thibault and Y. Arnaud. 1992. Micropropagation of pear (Pyrus communis L.): In Bajaj, Y.P.S. (ed.), Biotechnology of Perennial Fruit Crops, C.A.B. Intl. Wallingford, UK. pp. 263-276.
  19. Suzuki, M., T. Niino, T. Akihama, and S. Oka. 1997. Shoot formation and plant regeneration of vegetative pear buds cryopreserved at −150℃. J. Japan. Soc. Hort. Sci. 66:29-34. https://doi.org/10.2503/jjshs.66.29
  20. Singha, S. 1986. Pear (Pyrus communis): In Bajaj, Y.P.S. (ed.), Biotechnology in Agriculture and Forestry. Vol. I. Springer-Verlag, New York, USA. pp. 198-206.
  21. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant. 15:473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  22. Martin, P. 2003. Plant regeneration through direct somatic embryogenesis on seed coat explants of cashew (Anacardium occidentale L.). Sci. Hort. 98:299-304. https://doi.org/10.1016/S0304-4238(03)00005-0
  23. Scottez, C., E. Chevreau, N. Godard, Y. Arnaud, M. Duron and J. Dereuddre. 1992. Cryopreservation of cold-acclimated shoot tips of pear in vitro cultures after encapsulation-dehydration. Cryobiology 29:691-700. https://doi.org/10.1016/0011-2240(92)90073-B
  24. Reed, B.M. 1995. Screening Pyrus germplasm for in vitro rooting response. HortScience 30:1292-1294.
  25. Reed, B.M., J. Denoma, J. Luo, Y.J. Chang, and L. Towill. 1998. Cryopreservation and long-term storage of pear germplasm. In Vitro Cell Dev. Biol. Plant 34:256-260. https://doi.org/10.1007/BF02822718
  26. Pollard, J.W. and J.M. Walker. 1990. Plant cell and tissue culture: In Walker, J.M. (ed.), Methods in Molecular Biology, New Jersey (USA). pp. 81-91.
  27. Niino, T. and A. Sakai. 1992. Cryopreservation of alginate-coated in vitro-grown shoot tips of apple, pear and mulberry. Plant Sci. 87:199-206. https://doi.org/10.1016/0168-9452(92)90151-B
  28. Nemeth, G. 1986. Induction of rooting: In Bajaj, Y.P.S. (ed.), Biotechnology in Agriculture and Forestry-Trees I. Springer-Verlag, Berlin, Germany. pp. 49-64.
  29. Murashige, T. 1974. Plant propagation through tissue culture. Ann. Rev. Plant Physiol. 25:135-166. https://doi.org/10.1146/annurev.pp.25.060174.001031
  30. Zimmerman, R.H. and O.C. Broome. 1981. Phloroglucinol and in vitro rooting of apple cultivar cuttings. J. Ame. Soc. Hort. Sci. 106:648-652.
  31. Yeo, D.Y. and R.M. Reed. 1995. Micropropagation of three Pyrus rootstocks. HortScience 30:620-623.
  32. Vengadesan, G., A. Ganapathi, S. Amutha and N. Selvaraj. 2002. In vitro propagation of Acacia species-a review. Plant Sci. 163:663-671. https://doi.org/10.1016/S0168-9452(02)00144-9
  33. Torrey, J.G. 1976. Root hormones and plant growth. Ann. Rev. Plant Physiol. 27:435-459. https://doi.org/10.1146/annurev.pp.27.060176.002251
  34. Thakur, A., R.P.S. Dalal, and D. Navjot. 2008. Micropropagation of pear (Pyrus spp.): A review. Agric. Rev. 29:260-270.

피인용 문헌

  1. 1. Construction of high efficiency regeneration and transformation systems of Pyrus ussuriensis Maxim vol.131, pp.1, 2017, doi:10.7732/kjpr.2015.28.6.690

과제정보

연구 과제번호 : "사과, 배 유전자원의 초저온동결보존 기술개발 및 실용화"