Inhibition Activity of Plants on IgE-mediated Degranulation of RBL-2H3 Cells

Lee, Seung-Eun;Jeong, Hye-Gwang;Lee, Dae-Young;Lee, Jeong-Hoon;Choi, Jehun;Kim, Geum-Soog;Noh, Hyung-Jun;Lee, Jae-Won;Kim, Seung-Yu;Ahn, Young-Sup

  • 투고 : 2015.07.08
  • 심사 : 2015.11.25
  • 발행 : 2015.12.31


In this study, the inhibitory activities of fifty plant extracts on IgE-mediated degranulation in the rat basophilic leukemia cell line (RBL-2H3 cells) were measured; the release of interleukin (IL)-4 and β-hexosaminidase from IgE-sensitized cells treated with the plant extracts was measured; and the effects of the plant extracts on cell viability were tested. The results of the analysis of plant extracts at 20 μg/ml, including the aerial part of Magnolia sieboldii K. Koch, exhibited suppressive activities upon the release of IL-4. Furthermore, several plant extracts including methanol extracted from Lindera erythrocarpa Makino (aerial part) at the same concentration significantly inhibited the release of β-hexosaminidase. Twenty-six of the plant extracts, including methanol extract of Weigela subsessilis (Nakai) L. H. Bailey (branch), showed a cell proliferation effect of over 80% at 100 μg/ml. In conclusion, the results suggest that the leaf/stem of Geum japonicum Thunb. and the stamen/ovary of Nelumbo nucifera Gaertn., which exhibited effective inhibition on β-hexosaminidase release and IL-4 release from mast cells and showed high cell viability, could be useful candidates as anti-allergy materials.


Allergy;Degranulation;β-hexosaminidase;Mast cell;Interleukin-4


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