Barley Malt Treated with Enzymes Increases Polyphenol Content and Antioxidant Activity

DOI QR코드

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Phouthaxay, Phonesavanh;Yu, Chi Young;Pang, Yeon Gyu;Salitxay, Timnoy;Kim, Sang Heon;Park, Cheol Ho

  • 투고 : 2015.12.01
  • 심사 : 2015.12.24
  • 발행 : 2015.12.31

초록

The purpose of this study was to improve the functionality of a healthy drink with examining the possibility of manufacturing different enzymes (alpha-, beta-, glucose-amylase) in barley malts (BM) produced in various malting periods. The study showed that enzyme treatment increased significantly total polyphenol content (TPC), DPPH radical scavenging activity and hydroxly radical scavenging activity in malted liquid samples (MLS) which obtained from various malting periods. The highest of TPC were found in Gluco-24M with 1.981 mgTAE/ml, followed by Beta-24M and Alpha-72M with 1.878 mgTAE/ml and 1.845 mgTAE/ml, respectively. The DPPH result revealed that percent of inhibition increased by 71-75% compared to the control. No statistical difference was found between MLS obtained by 24 hr of malting (24 M) and 72 hr of malting (72 M) after enzyme treatment. In addition, an increasing of hydroxyl radical was in the same trend to the TPC and DPPH. The hydroxyl radical scavenging activity of enzyme treated samples was 1,5 times higher than the control. These results suggest the possibility of enzyme application to barley malts obtained in various germination periods for improving quality and functionality of barley malts.

키워드

Barley malt;Malting period;Enzymatic treatment;Antioxidant;Total polyphenol

참고문헌

  1. Bu, S.Y., W.B. Jeon, D.S. Kim, H.Y. Hoe and Y.W. Seo. 2002. Antioxidant activity and total phenolic compounds in grain extracts of wheat, barley, and oat. Korean J. Crop Sci. 47(2):201-207.
  2. Bryjak, J. 2003. Gluco-amylase, alpha-amylase and beta-amylase immobilisation on acrylic carriers. Biochemical Engineering Journal 16:347-355. https://doi.org/10.1016/S1369-703X(03)00114-1
  3. Braca, A.G., I. Morelli, F. DeiSimone and N.D. Tommasi. 2003. Antioxidant and free radical scavenging activity of flavonol glycosides from different Aconitum species. J. Ethnopharmacol. 86:63-67. https://doi.org/10.1016/S0378-8741(03)00043-6
  4. Beta, T., S. Nam, J.F. Dexter and H.D. Sapirstein. 2005. Phenolic content and antioxidant activity of pearled wheat fraction. Cereal Chemistry 82(4):390-393. https://doi.org/10.1094/CC-82-0390
  5. Lee, N.Y., Y.K. Kim, I. Choi, S.K. Cho, H.J. Kweon, K.W. Lee, J.N. Hyun, J.S. Choi, K.H. Park, K.J. Kim and M.J. Lee. 2010. Antioxidant activity and biological activity of specialty barley (Horduem vulgare L.) extracts. Korean J. Crop Sci. 55(2):159-164.
  6. Lee,Y.T., S.J. Seo and H.G. Chang. 1999. Quality characteristics of barley varieties related to enzymatic activity in malt. Korean J. Food Sci. Technol. 31(6):1421-1426 (in Korean).
  7. Laseken, O.O. 1999. Effect of Vernonai amygdalina (bitter leaf) extract on brewing qualities and amino acid profiles of stout drinks from sorhum and barley malts. Food Chem. 64:507-510. https://doi.org/10.1016/S0308-8146(98)00148-4
  8. Halliwell, B., J.M.C.Guttridge and O.I. Aruoma. 1987. Analytical Biochemistry 165:215-219. https://doi.org/10.1016/0003-2697(87)90222-3
  9. Jang, H.J., H.J. Bu and S. Lee. 2015. Screening for antioxidative activity of Jeju native plants. Korean J. Plant Res. 28(2):158-167. https://doi.org/10.7732/kjpr.2015.28.2.158
  10. Fogarasi, A.L. 2015. A comparative assessment of antioxidant properties, total phenolic content of einkorn, wheat, barley and their malts. Food Chem. 167:1-6. https://doi.org/10.1016/j.foodchem.2014.06.084
  11. Evers, A.D. and E.E. McDermot. 1970. Scanning electron microscopy of wheat starch. II. Structure of granules modified by α-amylolysis-preliminary report. Staerke 22:23. https://doi.org/10.1002/star.19700220107
  12. Cook, N.C. and S. Samman. 2006. Flavonoids-chemistry, metabolism, cardio protective effect, and dietary sources. Nutr. Biochem. 7:66-76.
  13. Chon, S.U and Y.M. Kim. 2006. Phytotoxicity and DPPH radical scavenging activity of barley seedling extracts. Korean J. Crop Sci. 51(4):322-328.
  14. Chon, S.U., T.S. Kim, J.S. Shin and H.O. Boo. 2008. Antioxidant activity of methanol extracts from root part of Korean salad plants. Korean J. Plant Res.12(6):413-419.
  15. Zielinski, H. and H. Kozlowska. 2000. Antioxidant activity and total phenolics in selected cereal grains and their different morphological fraction. J. Agric. Food Chem. 48:2008-2016. https://doi.org/10.1021/jf990619o
  16. Singleton, V.L. and J.A. Rossi. 1965. Calorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. American J. of Enology and Viticulture 16:144-158.
  17. Seog, H.M., M.S. Seo, S.R. Kim., Y.K. Park and Y.T. Lee. 2002. Characteristics of barley polyphenol extract (BPE) separated from pearling by-products. Korean J. Food Sci. Technol. 34(5):775-779 (in Korean).
  18. Ryu, S.N., S.Z. Park and H.Y. Kim. 2002. Antioxidant activity in rice cultivar, wild rice, and barley. Korean J. Crop Sci. 47(1):54-61.
  19. Qingming, Y., P. Xianhui, K. Weiboa,Y. Hong and S.Yidan. 2010. Antioxidant activity of malt extract from barley (Hordeum vulgare L.) toward various oxidative stress in vitro and in vivo. Food Chem. 188:84-89.
  20. Maillard, M.N., M.H. Soum, P. Boivin and C. Berset. 1996. Antioxidant activity of barley and malt: Relationship with phenolic content. Lebensmittel Wissenschaft and Technologie. 29:238-244. https://doi.org/10.1006/fstl.1996.0035