DOI QR코드

DOI QR Code

Antioxidant and Antibacterial Activity of Water Soluble Polysaccharide from Defatted Botryococcus braunii

탈지 미세조류 Botryococcus braunii 로부터의 수용성 다당의 항산화 활성과 항균활성

  • Received : 2015.12.28
  • Accepted : 2016.01.27
  • Published : 2015.12.31

Abstract

Sulfated polysaccharides from plants or seaweed are well known for antioxidant, anticoagulant activity or other biological activities. The aim of the present study is to optimize the condition for the isolation of water soluble polysaccharides (WSP) and evaluate the biological activity. WSP was isolated from defatted microalgae B. braunii using chlorosulfonic acid or sulfuric acid. Among WSP isolated using sulfuric acid showed the lowest the content of total carbohydrates (g/L), whereas showed the highest antioxidant activity. Also the preliminary structural analysis of WSP was performed by FT-IR spectroscopy analysis.

Keywords

Botryococcus braunii;Chlorosulfonic acid;Sulfuric acid;Antioxidant activity

References

  1. Alban, S., Schauerte, A. and Franz, G. 2002. Anticoagulan t sulfated polysaccharides: Part I. Synthesis and structure -activity relationships of new pullulan sulfates. Carbohyd. polym. 47, 267-276. https://doi.org/10.1016/S0144-8617(01)00178-3
  2. Gomez-Ordonez, E., Jimenez-Escrig, A. and Ruperez, P. 2014. Bioactivity of sulfated polysaccharides from the edible red seaweed Mastocarpus stellatus. Bioact. Carbo hydr. Dietary Fibre, 3, 29-40. https://doi.org/10.1016/j.bcdf.2014.01.002
  3. Sellimi, S., Kadri, N., Barragan-Montero, V., Laouer, H., Hajji, M. and Nasri, M. 2014. Fucans from a Tunisian brown seaweed Cystoseira barbata: Structural characteristic and antioxidant activity. Int. J. Biol. Macromol., 66, 281-288. https://doi.org/10.1016/j.ijbiomac.2014.02.041
  4. Wu, Y., Cui, S. W., Tang, J. and Gu, X. H. 2007. Optimization of extraction process of crude polysaccharides from boat-fruited sterculia seeds by response surface methodology. Food Chem., 105, 1599-1605. https://doi.org/10.1016/j.foodchem.2007.03.066
  5. Wang, X., Zhang, Z., Yao, Z., Zhao, M. and Qi, H. 2013. Sulfation, anticoagulant and antioxidant activities of poly saccharide from green algae Enteromorpha linza. Int. J. Biol. Macromol., 58, 225-230. https://doi.org/10.1016/j.ijbiomac.2013.04.005
  6. Wang, X., Wang, J., Zhang, J., Zhao, B., Yao, J. and Wang, Y. 2010. Structure-antioxidant relationships of sulfated galactomannan from guar gum. Int. J. Biol. Macro mol., 46, 59-66. https://doi.org/10.1016/j.ijbiomac.2009.10.004
  7. Telles, C. B. S., Sabry, D. A., Almeida-Lima, J., Costa, M. S. S. P., Melo-Silveira, R. F., Trindade, E. S., Sassaki, G. L., Wisbeck, E., Furlan, S. A., Leite, E. L. and Rocha, H. A. O. 2011. Sulfation of the extracellular poly saccharide produced by the edible mushroom Pleurotussajor-caju alters its antioxidant, anticoagulant and antipr oliferative properties in vitro. Carbohyd. polym., 85, 514-521. https://doi.org/10.1016/j.carbpol.2011.02.038
  8. Lu, Y., Wang, D. Y., Hu, Y. L., Huang, X. Y. and Wang, J. M. 2008. Sulfated modification of epimedium polysac charide and effects of the modifiers on cellular infectivit y of IBDV. Carbohyd. polym., 71, 180-186. https://doi.org/10.1016/j.carbpol.2007.05.024
  9. Chen, Y., Zhang, H., Wang, Y., Nie, S., Li, C. and Xie, M. 2015. Sulfated modification of the polysaccharides from Ganoderma atrum and their antioxidant and immun omodulating activities. Food Chem., 186, 231-238. https://doi.org/10.1016/j.foodchem.2014.10.032
  10. Wang, Y., Peng, Y., Wei, X., Yang, Z., Xiao, J. and Jin, Z. 2010. Sulfation of tea polysaccharides: Synthesis, characterization and hypoglycemic activity. Int. J. Biol. Macromol., 46, 270-274. https://doi.org/10.1016/j.ijbiomac.2009.12.007
  11. Hussein, M. M., Helmy, W. A. and Salem, H. M. 1998. Biological activities of some galactomannans and their sulfated derivatives. Phytochemistry, 48, 479-484. https://doi.org/10.1016/S0031-9422(98)00024-7
  12. Jian-Ya, Q., Ye-Yu, B., Jing, T. and Wei, C. 2015. Antio xidation and ${\alpha}$-glucosidase inhibitory activites of barley polysaccharides modified with sulfation. LWT-Food Sci. Technol., 64, 104-111.
  13. Lu, X., Mo, X., Guo, H. and Zhang, Y. 2012. Sulfation modification and anticoagulant activity of the polysaccha rides obtained from persimmon (Diospyros kaki L.) fruits. Int. J. Biol. Macromol., 51, 1189-1195. https://doi.org/10.1016/j.ijbiomac.2012.08.028
  14. Geresh, S., Mamontov, A. and Weinstein, J. 2002. Sulfati on of extracellular polysaccharides of red microalgae: preparation, characterization and properties. J. Biochem. Biophys. Methods., 50, 179-187. https://doi.org/10.1016/S0165-022X(01)00185-3
  15. Al-Hothaly, K. A., Adetutu, E. M., Taha, M., Fabbri, D., Lorenzetti, C., Conti, R., May, B. H., Shar, S. S., Bayoumi, R. A. and Ball, A. S. 2015. Bio-harvesting and pyrolysis of the microalgae Botryococcus braunii. Bioresour. Technol., 191, 117-123. https://doi.org/10.1016/j.biortech.2015.04.113
  16. Yang, X. B., Gao, X. D., Han, F. and Tan, R. X. 2005. Sulfation of a polysaccharide produced by a marine filamentous fungus Phoma herbarum YS4108 alters its antioxidant properties in vitro. Biochim. Biophys. Acta, 1725, 120-127. https://doi.org/10.1016/j.bbagen.2005.06.013
  17. Lee, C. G., Lee, J., Lee, D. G., Kim, J. W., Alnaeeli, M., Park, Y. I. and Park, J. K. 2016. Immunostimulating activity of polyhydric alcohol isolated from Taxus cuspidata. Int. J. Biol. Macromol., 85, 505-513. https://doi.org/10.1016/j.ijbiomac.2016.01.027
  18. Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A. and Smith, F. 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem., 28, 350-356. https://doi.org/10.1021/ac60111a017
  19. Mohsin, S., Mahadevan, R. and Kurup, G. M. 2014. Free-radical-scavenging activity and antioxidant effect of ascophyllan from marine brown algae Padina tetrastromatica. Biomed. Prev. Nutr., 4, 75-79. https://doi.org/10.1016/j.bionut.2013.08.006
  20. Zheng, L., Zhao, M., Xiao, C., Zhao, Q. and Su, G. 2016. Practical problems when using ABTS assay to assess the radical-scavenging activity of peptides: Importance of controlling reaction pH and time. Food Chem., 192, 288-294. https://doi.org/10.1016/j.foodchem.2015.07.015
  21. Goo, B. G., Baek, G., Choi, D. J., Park, Y. I., Synytsya, A., Bleha, R., Seong, D. H., Lee, C. G. and Park, J. K. 2013. Characterization of a renewable extracellular polysaccharide from defatted microalgae Dunaliella tertiolecta. Bioresour. Technol., 129, 343-50. https://doi.org/10.1016/j.biortech.2012.11.077

Acknowledgement

Supported by : 해양수산부