Determination of phenol using solid-phase extraction and HPLC/MSD/FLD in water

고체상추출법과 HPLC/MSD/FLD를 이용한 수질중의 페놀 분석

  • 이태준 (강원도보건환경연구원) ;
  • 박근영 (강원도보건환경연구원) ;
  • 표동진 (강원대학교 자연과학대학 화학과)
  • Received : 2015.08.11
  • Accepted : 2015.10.27
  • Published : 2015.12.25


An analytical method for determining phenol considered priority pollutants of the US EPA and precursor of toxic phenolic compounds by solid-phase extraction (SPE) and high performance liquid chromatographic systems (HPLC) equipped with fluorescence and mass selective detectors have been developed. The SPE process for sample preconcentration was performed on a commercially available Oasis HLB cartridge packed with polymeric sorbents. The effect of pH, elution solvent, and elution volume on the recoveries of the analytes were investigated with HPLC/FLD. Average recovery of >87.0% was achieved with 60 mg sorbents using 5 mL of methanol as an elution solvent at pH=3.


phenol;hplc;solid phase extraction(spe);hplc/msd


  1. J. Pastsias and E. Papadopoulou-Mourkidou, J. Chromatogr. A, 904, 171-188 (2000).
  2. M. Kawaguchi, K. Inoue, M. Yoshimura, R. Ito, N. Sakui and H. Nakazawa, Anal. Chim. Acta, 505, 217-222 (2004).
  3. N. Masque, M. Galia, R. M. Marce and F. Borrull, J. Chromatogr. A, 771, 55-61 (1997).
  4. Ministry of Envirnment, “Integrated Environmental Law”, Daeyang Press, 2001.
  5. R. Wissiack, E. Rosenberg and M. Grasserbauer, J. Chromatogr. A, 896, 159-170 (2000).
  6. E. Gonzalz-Toledo, M. D. Prat and M. F. Alpendurada, J. Chromatogr. A, 923, 45-52 (2001).
  7. D. Puig and D. Barcelo, J. Chromatogr. A, 733, 371-381 (1996).
  8. H. Bagheri and A. Saber, S. R. Mousavi, J. Chromatogr. A, 1046, 27-33 (2004).
  9. An Venrmeulen, Karien Welvaert and Joeri Vercammen, J. Chromatogr. A, 1071, 41-46 (2005).
  10. EPA Method 8041, “Phenols by Gas chromatography : Capillary column technique”, Environmental Protection Agency, Washington, DC, 1996.
  11. V. Pichon and M. C. Hennion, J. Chromatogr., A, 665, 269-281 (1994).
  12. D. Barcelo and M. C. Hennion, Anal. Chim. Acta, 318, 1-41 (1995).
  13. A. Penalver, E. Pocurull, F. Borrul and R. M. Marce, J. Chromatogr. A, 953, 79-87 (2002).
  14. P. Pocurull, R. M. Marce, F. Borrull, J. L. Bennal, L. Toribio and M. L. Serna, J. Chromatogr. A, 755, 67-74 (1996).
  15. P. D. McDonald and E. S. P. Bouvier, “Solid phase extraction application guide and bibiography”, 6th Eds., Waters, Milford, Massachusetts, USA. 1995.
  16. P. Lee Ferguson, C. R. Iden and B. J. Brownawell, Anal. Chem., 72, 4322-4330 (2000).