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Establishment of analytical methods for HPHC list of mainstream cigarette smoke

담배 주류연 중 7개 그룹의 유해성분(HPHC) 분석법 확립 및 유효성 평가

  • Park, Hyoung-Joon (Advanced Analysis Team, Toxicological Evaluation and Research Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Lee, Jin-Hee (Advanced Analysis Team, Toxicological Evaluation and Research Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Cho, So-Hyun (Advanced Analysis Team, Toxicological Evaluation and Research Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Heo, Seok (Advanced Analysis Team, Toxicological Evaluation and Research Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Yoon, Chang-yong (Advanced Analysis Team, Toxicological Evaluation and Research Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Baek, Sun-Young (Advanced Analysis Team, Toxicological Evaluation and Research Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
  • 박형준 (식품의약품안전처 식품의약품안전평가원 첨단분석팀) ;
  • 이진희 (식품의약품안전처 식품의약품안전평가원 첨단분석팀) ;
  • 조소현 (식품의약품안전처 식품의약품안전평가원 첨단분석팀) ;
  • 허석 (식품의약품안전처 식품의약품안전평가원 첨단분석팀) ;
  • 윤창용 (식품의약품안전처 식품의약품안전평가원 첨단분석팀) ;
  • 백선영 (식품의약품안전처 식품의약품안전평가원 첨단분석팀)
  • Received : 2015.02.23
  • Accepted : 2015.11.17
  • Published : 2015.12.25

Abstract

Harmful and potentially harmful constituents (HPHCs) are chemical compounds in tobacco smoke that cause harm to smokers and non-smokers. This study established and validated methods for the analysis of HPHCs from mainstream cigarette smoke. The analyzed HPHCs were categorized into seven groups: aromatic amines, volatile organic compounds (VOCs), heavy metals, tobacco specific nitrosamines (TSNAs), benzo[a]pyrene (B[a]P), ammonia, and carbonyl compounds. The methods were validated by specificity, linearity, limit of detection (LOD), accuracy, precision, and recovery. These validated methods were then applied to the reference cigarettes (1R5F, 3R4F). The correlation coefficients (r2) for the calibration curves of the seven groups were over 0.995. The LODs showed values of 0.01-0.04 ng/cig cig for aromatic amines, 0.01-0.16 μg/cig for VOCs, 0.01-1.27 ng/cig for heavy metals, 0.06-0.28 ng/cig for TSNAs, 0.04 ng/cig for benzo[a]pyrene, 0.08 μg/cig for ammonia, and 0.78-1.77 μg/cig for carbonyl compounds. The precisions obtained from the intra and inter-day batches were less than 15%. The accuracy and the recovery range were less than 15% and 79.2-117.5%, respectively. The proposed methods can therefore be applied for determining HPHCs in tobacco mainstream smoke.

Keywords

1R5F;3R4F;cigarette;HPHCs

References

  1. R. Doll and A. B. Hill, Br. Med. J., 2, 739-748 (1950). https://doi.org/10.1136/bmj.2.4682.739
  2. E. L. Wynder and E. A. Graham, J. Am. Med. Assoc., 143, 329-336 (1950). https://doi.org/10.1001/jama.1950.02910390001001
  3. IARC, In 'International agency for research on cancer', 83 (2004).
  4. WHO, In ‘WHO report on the global tobacco epidemic’, Mpower, (2002).
  5. S. S. Hecht, Environ. Mol. Mutagen, 39, 119-126 (2002). https://doi.org/10.1002/em.10071
  6. C. R. Green and A. Rodgman, Recent Adv. tob. Sci, 22, 131-304 (1996).
  7. S.-J. Kim and S.-M. Kwon, The Korean Assoc. Policy Analysis and Evaluation, 18(4), 119-140 (2008).
  8. S. Purkis and M. Intorp, Beitr Zur Tabakforsch, 26(2), 57-73 (2014).
  9. E. Roemer, H. Schramke, H. Weiler, A. Buetter, S. Kausche, S. Weber, A. Berges, M. Stueber, M. Muench, E. Trelles-Sticken, J. Pype, K. Kohlgrueber, H. Voelkel and S. Wittke, Beitr Zur Tabakforsch, 25(1), 316-335 (2014).
  10. UK SMOKE CONSTITUENTS STUDY, In ‘Part 11: Determination of Metals Yields in Cigarette Smoke By ICP-MS & CVAAS’, (2003).
  11. M. Intorp, W. S. Purkis and W. Wagstaff, Beitr Zur Tabakforsch, 24(5), 243-251 (2014).
  12. C. Patriankos and D. Hoffmann, J. Anal. Toxicol, 3, 150-154 (1979). https://doi.org/10.1093/jat/3.4.150
  13. ISO, 3308 : 2000(E) (2000).
  14. H.-K. Kim, J.-T. Lee, I.-J. Kim and K.-J. Hwang, J. Kor. Soc. Tobacco., 9, 52-57 (2007).
  15. M. Intorp and S. Purkis, Beitr Zur Tabakforsch., 24, 78-92 (2014).
  16. British American Tobacco Group Research & Development Method, In ‘Determination of carbonyls in mainstream cigarette smoke’, (2008).
  17. Health Canada, T-101 (1999).
  18. British American Tobacco Group Research & Development Method, In ‘Determination of benzo[a]pyrene in mainstream smoke’, (2008).
  19. CORESTA, No.75 (2012).
  20. Health Canada, T-109 (1999).
  21. CORESTA, No.70 (2010).
  22. Health Canada, T-202 (1999).
  23. M. Intorp, S. Purkis and W. Wagstaff, Rapid Commun. Mass Spectrom, 25(2), 361-374 (2014).
  24. M. Intorp, S. Purkis, M. Whittaker and W. Wright, Beitr Zur Tabakforsch, 23(4), 161-202 (2014).
  25. UK smoke constituents study. In ‘Part 4 method: determination of benzo[a]pyrene yields in mainstream cigarette smoke by gas chromatography-mass spectrometry’, (2003).