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A study of relationship between stomach cancer and selenoproteins in Korean human blood serum

한국인 혈청에서의 셀레노 단백질과 위암과의 상관관계 연구

  • Park, Myungsun (Department of Chemistry Education, Korea National University of Education) ;
  • Pak, Yong-Nam (Department of Chemistry Education, Korea National University of Education)
  • 박명순 (한국교원대학교 화학교육학과) ;
  • 박용남 (한국교원대학교 화학교육학과)
  • Received : 2015.10.05
  • Accepted : 2015.12.01
  • Published : 2015.12.25

Abstract

In this study, the relationship between selenoprotein concentrations in blood and stomach cancer have been searched for Korean. The concentration of each selenoprotein in blood serum was analyzed and the correlation between the concentration and stomach cancer was studied to find a potential for using Selenium as a biomarker. In concentration determination, a simple calibration curve method was used with the monitoring of m/z 78 without the use of solid phase extraction. This is a lot more simple than the method using SPE with post column isotope dilution. The result obtained from the analysis of CRM BCR-637, 72.20±3.35 ng·g−1, showed similar value of reference value (81±7 ng·g−1). The total concentration of Se for the controlled group, cardiovascular patients group, was 105.70±21.20 ng·g−1. This value was the same as normal healthy person reported earlier. Each selenoprotein concentration of GPx, SelP and SeAlb was 26.12±7.84, 65.15±14.50, 14.43±6.99 ng·g−1, respectively. The distribution of each selenoprotein was 24.7%, 61.6%, and 13.7%, which was similar to the normal person. The result of stomach cancer patients, the total concentration of Se was 76.11±28.12 ng·g−1 and each concentration of GPx, SelP and SeAlb was 15.41±9.01, 50.83±17.91, and 9.87±5.21 ng·g−1, respectively. The total and each selenoprotein concentration level showed significant decrease for the stomach cancer patients. The level of decrease was 41.0% for GPx, 22.0% for SelP, and 31.6% for SeAlb. However, the distribution of each selenoprotein was not much different. Either total Selenium or each selenoprotein could be used as a possible index for the diagnosis of cancer. However, in age group study, it is shown that young age group (30's-40's) did not show much difference.

Keywords

stomach cancer;selenoproteins;Korean blood serum;AF HPLC-ICP/MS;GPx;SelP;SeAlb

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