Development of a cholesterol biosensor modified with carbon nanotube

탄소나노튜브를 이용하여 개조한 콜레스테롤 바이오 센서 개발

  • Kim, Haidong (Department of Chemistry, Kyung Hee University)
  • Received : 2015.10.06
  • Accepted : 2015.10.27
  • Published : 2015.12.25


A cholesterol biosensor was developed using a modified carbon electrode with carbon nanotubes. The disposable cholesterol biosensor was modified with carbon nanotubes to enhance electron transfer during the enzymatic reaction of cholesterol. Cholesterol oxidase and peroxidase, with potassium ferrocyanide as a mediator, were immobilized on a screen-printed carbon nanotube electrode. The electrochemical cholesterol biosensor developed using carbon nanotubes showed a rapid and reliable signal for measuring total cholesterol. The cholesterol sensor showed a linear response in 5 seconds with a small volume (0.5 μL) in the range of 100~400 mg/dL, with a coefficient of variation of 4.0%.


cholesterol;point-of-care;blood test


  1. J. Wang, Electroanal., 17, 7-14 (2005).
  2. H. Chengguo and H. Shenghui, J. Sensors, 1-40 (2009).
  3. J. L. Yi, D. K. Jung and Y. P. Jae, J. Kor. Phys. Soc., 54, 1769-1773 (2009).
  4. F. Ricci and G. Palleschi, Biosens. Bioelectron, 21, 389-407 (2005).
  5. S. Park, H. Boo and T. D. Chung, Anal. Chim. Acta, 556, 46-57 (2006).
  6. Y. Wang, X. Hui, J. Zhang and L. Guang, Sensors, 8, 2043-2081 (2008).
  7. M. Barbadillo, E. Casero, Petit-Dominguez, M. D., L. Vazquez, F. Pariente and E. Lorenzo, Talanta, 80, 797-802 (2009).
  8. Y. Y. Hosokawa, H. Hakamata, T. Murakami, S. Aoyagi, M. Kuroda, Y. Mimaki, Y. A. Ito, S. Morosawa and F. Kusu, Electrochim. Acta, 54, 6412-6416 (2009)
  9. B. Canabate-Diaz, C. A. Segura, A. F. Gutierrez, V. A. Belmonte, F. A. Garrido, V. J. L. Martinez and M. J. Duran, Food Chem, 102, 593-598 (2007).
  10. C. K Pires, B. F. Reis, C. X. Galhardo and P. B. Martelli, Anal. Lett., 36, 3011-3024 (2003).
  11. J. B. Claver, J. A. Cabello, J. M. Sanfrutos, A. M. Fernandez, M. C. V. Miron, F. S. Gonzalez and L. F. C. Vallvey, Anal. Chim. Acta, 754, 91-98 (2012).
  12. O. Turkarslan, S. K. Kayahan and L. Toppare, Sens. Actuators B, 136, 484-488 (2009).
  13. G. R. Warnick, M. M. Kimberly, P. P. Waymack and E. T. Leary, Labmedicine, 39(8), 481-490 (2008).
  14. S. K. Arya, M. Datta and B. D. Malhotra, Biosens. Bioelectron., 23, 1083-1100 (2008).
  15. M. Q. Israr1, K. Hasan, J. R. Sadaf, I. Engquist, O. Nur, M. Willander and B. Danielsson, J. Biosens Bioelectron, 2(3), 1000109 (2011).