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Analysis of volatile compounds and metals in essential oil and solvent extracts of Amomi Fructus

사인으로부터 추출한 정유와 용매 추출물의 휘발성 물질 및 금속성분 분석

  • Lee, Sam-Keun (Department of Applied Chemistry, Daejeon University) ;
  • Eum, Chul Hun (Korea Institute of Geoscience and Mineral Resources) ;
  • Son, Chang-Gue (Liver & Immunology Research Center, Oriental Hospital of Daejeon University)
  • 이삼근 (대전대학교 응용화학과) ;
  • 음철헌 (한국지질자원연구원 지질자원분석센터) ;
  • 손창규 (대전대학교 한의과 대학)
  • Received : 2015.11.03
  • Accepted : 2015.11.13
  • Published : 2015.12.25

Abstract

Amomi Fructus with anti-oxidative activity was chosen and essential oil was obtained by SDE (simultaneous distillation extraction), and 39 constituents were determined by GC-MS (gas chromatography-mass spectrometry). Major components were camphor, borneol acetate, borneol, D-limonene and camphene. Three solvent extracts such as hexanes, diethyl ether and methylene chloride from Amomi Fructus were obtained. These were analyzed by GC-MS and 4 more constituents were identified in addition to 39 components discovered in essential oil. Five major components such as camphor, borneol acetate, borneol, D-limonene and camphene were also detected, however the relative peak percents of those components were different from those of constituents in essential oil. To estimate the kind and the amount of materials evaporated at certain temperature and conditions from essential oil and solvent extracts, dynamic headspace apparatus was used and materials evaporated and trapped at certain conditions were analyzed by GC-MS. Recovery yield of SDE method from Amomi Fructus was measured by using camphor and standard calibration solution of camphor methanol solution and, the yield was 82.0%. Content of Hg was measured by mercury analyzer and contents of Cd, Pb, Cr, Mn, Co, Ni, Cu and Zn in Amomi Fructus, essential oils and solvent extracts were determined by ICP-MS (Inductively coupled plasma-mass spectrometer). Pb, Cd and Hg were measured in the concentration of 0.72 mg/kg, <0.10 mg/kg and 0.0023 mg/kg, respectively and these were below permission level of purity test. Contents of Mn, Cu and Zn in Amomi Fructus were 213 mg/kg, 8.29 mg/kg and 31.0 mg/kg, respectively and which were relatively higher than other metals such as Cr, Co and Ni. Metals such as Mn (0.65 ~ 9.08 mg/kg), Cu (1.16 ~ 4.40 mg/kg) and Zn (1.10 ~ 3.80 mg/kg) in essential oil and solvent extracts were detected. At this point it is not clear that the metals were cross-contaminated in the course of treating Amomi Fructus or metals were contained in Amomi Fructus. The influence evaluation toward biological model study of these metals in essential oil and solvent extracts will be needed.

Keywords

Amomi Fructus;simultaneous distillation extraction;essential oil;solvent extraction;dynamic headspace/GC-MS

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