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Effect of microorganisms collected from uterus of Hanwoo cattle with low conception rate on the development of IVF-derived embryos

저수태 한우 암소 자궁에서 회수된 미생물이 체외수정란 발달율에 미치는 영향

Woo, Jae-Seok;Kim, Ki-Hyun;Cho, Eun-Seok;Yeon, Seong-Heum;Park, Youn-Bae;Kim, Min-Kyu;Sa, Soo-Jin
우제석;김기현;조은석;연성흠;박연배;김민규;사수진

  • Received : 2015.07.15
  • Accepted : 2015.09.07
  • Published : 2015.12.31

Abstract

The cause of infertility is either fertilization failure or early embryonic death. The aetiology may involve a combination of many factors, e.g. genetic factors, abnormalities in the gametes nutritional disorders, inadequate luteal function, and delayed ovulation. This study was conducted to investigate the effect of microorganisms collected from uterus of Hanwoo cattle on early embryonic development. Microorganisms isolated from the uterus of Hanwoo cattle included Bacillus cereus (Bc), Bacillus thuringiensis (Bt), Staphylococcus warneri (Sw) and Enterococcus faecalis (Ef). When cultured with Bc, Bt, Sw, and Ef, oocytes were not developed into a blastocyst in vitro. The proportion of blastocyst was dramatically increased after reducing the number of microorganisms ($10^4CFU/ml$). Interestingly, the proportion of blastocyst was decreased by adding the Sw and Ef. These results indicate that among intrauterine microorganisms, Sw and Ef strains negatively affect theearly embryonic development, thereby aggravate the rates of implantation and pregnancy. These findings will provide useful information for association studies in other pig populations.

Keywords

Cattle;Microorganisms;Early embryonic development;Uterus

References

  1. Ahlers D, Grunert E. 1993. Zur Problematik der Behandlung des infizierten Uterus beim Rind im Puerperium. Prakt Tierarzt Sonderh Coll Vet 24:57-62.
  2. Akhtar M, Singh B. 1979. Livability and fertility rate of spermatozoa in bovine cervical mucus under normal and disease conditions [dairy cattle, India]. Indian Veterinary Journal (India).
  3. Announ N, Mattei JP, Jaoua S, Fenollar F, Sati H, Chagnaud C, Roudier J, Guis S. 2004. Multifocal discitis caused by Staphylococcus warneri. Joint Bone Spine 71(3):240-242. https://doi.org/10.1016/S1297-319X(03)00126-X
  4. Barigye R, Schaan L, Gibbs PS, Schamber E, Dyer NW. 2007. Diagnostic evidence of Staphylococcus warneri as a possible cause of bovine abortion. Journal of veterinary diagnostic investigation 19(6):694-696. https://doi.org/10.1177/104063870701900613
  5. Boland M, Crosby T, Gordon I. 1976. Birth of twin calves following a simple transcervical non-surgical egg transfer technique. Veterinary Record 99(14):274-275.
  6. Broadbent P, Stewart M, Dolman D. 1991. Recipient management and embryo transfer. Theriogenology 35(1):125-139. https://doi.org/10.1016/0093-691X(91)90152-4
  7. Brock T, Madigan MT, Martinko J, Parker J. 2009. Brock's Biology of Microorganisms. Pearson Benjamin Cummings Press: San Francisco, CA, USA.
  8. Christensen LG. 1991. Use of embryo transfer in future cattle breeding schemes. Theriogenology 35(1):141-149. https://doi.org/10.1016/0093-691X(91)90153-5
  9. Donaldson L. 1985. Matching of embryo stages and grades with recipient oestrous synchrony in bovine embryo transfer. The Veterinary Record 117(19):489-491. https://doi.org/10.1136/vr.117.19.489
  10. Hussain A, Daniel R. 1992. Phagocytosis by uterine fluid and blood neutrophils and hematological changes in postpartum cows following normal and abnormal parturition. Theriogenology 37(6):1253-1267. https://doi.org/10.1016/0093-691X(92)90181-P
  11. Jainudeen M, Hafez E. 1993. Reproductive failure in females. Reproduction In Farm Animals 6th ed Lea & Febiger, Philadelphia: 261-286.
  12. Kim S, Park Y, Park H, Park H, Kim J. 2005. Effect of Embryo Factors on Pregnancy and Abortion Rate after Transfer of In Vitro Produced Korean Native Cattle Embryos. Korean Journal of Embryo Transfer. 20(1):17-24. [in Korean]
  13. Lafi S, Kaneene J. 1992. Epidemiological and economic study of the repeat breeder syndrome in Michigan dairy cattle. I. Epidemiological modeling. Preventive Veterinary Medicine 14(1):87-98. https://doi.org/10.1016/0167-5877(92)90087-V
  14. Laven R, Peters A. 1996. Bovine retained placenta: aetiology, pathogenesis and economic loss. The Veterinary Record 139(19):465-471. https://doi.org/10.1136/vr.139.19.465
  15. Leighton P, Little J. 1986. Identification of coagulase-negative Staphylococci isolated from urinary tract infections. American journal of clinical pathology 85(1):92-95. https://doi.org/10.1093/ajcp/85.1.92
  16. Mellado M, Reyes C. 1994. Associations between periparturient disorders and reproductive efficiency in Holstein cows in northern Mexico. Preventive Veterinary Medicine 19(3):203-212. https://doi.org/10.1016/0167-5877(94)90089-2
  17. Nelson LD, Nelson CF. 1985. Effect of estrus detection and corpus luteum development on pregnancy rates in bovine embryo recipients. Theriogenology 23(1):212. https://doi.org/10.1016/0093-691X(85)90118-9
  18. Ray C. 2004. Sherris medical microbiology. McGraw Hill.
  19. Semambo D, Eckersall P, Sasser R, Ayliffe T. 1992. Pregnancyspecific protein B and progesterone in monitoring viability of the embryo in early pregnancy in the cow after experimental infection with Actinomyces pyogenes. Theriogenology 37(3):741-748. https://doi.org/10.1016/0093-691X(92)90153-I
  20. Singh J, Sidhu S, Dhaliwal G, Pangaonkar G, Nanda A, Grewal A. 2000. Effectiveness of lipopolysaccharide as an intrauterine immunomodulator in curing bacterial endometritis in repeat breeding cross-bred cows. Animal reproduction science 59(3):159-166. https://doi.org/10.1016/S0378-4320(00)00144-5
  21. Sreenan J. Methods of consistent supply, recovery and transfer of embryos in cattle; 1983. p 197-212.
  22. Sreenan J, Diskin M. 1987. Factors affecting pregnancy rate following embryo transfer in the cow. Theriogenology 27(1):99-113. https://doi.org/10.1016/0093-691X(87)90073-2
  23. Walton J, Martineau N, Stubbings R. 1986. Pregnancy rates in Holstein embryo transfer recipients: effect of treatment with progesterone or clenbuterol and of natural versus induced cycles. Theriogenology 26(6):837-845. https://doi.org/10.1016/0093-691X(86)90013-0
  24. Wright JM. 1981. Non-surgical embryo transfer in cattle embryorecipient interactions. Theriogenology 15(1):43-56. https://doi.org/10.1016/S0093-691X(81)80017-9

Acknowledgement

Supported by : 농촌진흥청