Biogenic Amine Contents of Commercial Salted and Fermented Sand Lance Ammodytes personatus Sauces

시판 까나리(Ammodytes personatus) 액젓의 biogenic amines 함량

Um, In-Seon;Park, Kwon-Sam

  • Received : 2015.10.14
  • Accepted : 2015.12.14
  • Published : 2015.12.31


This study investigated the quality of seven commercial salted and fermented sand lance Ammodytes personatus sauces by measuring their chemical compositions, bacteria concentrations, and biogenic amine contents. The sauces had a 63.97-67.32% (mean: 65.72%) moisture content, 23.16-24.03% (mean: 23.60%) salinity, 5.51-6.14 (mean: 5.81) pH, 164.76-217.98 mg/100 g (mean: 182.97 mg/100 g) volatile basic nitrogen, 0.94-1.42% (mean: 1.24%) total nitrogen, and 673.77-791.86 mg/100 g (mean: 720.76 mg/100 g) amino nitrogen content. Viable cell counts ranged from $1.2{\times}10^1$ to $2.9{\times}10^2CFU/mL$, and number of biogenic amine-forming bacteria was very low or not detected. Overall, the tested samples had average levels of histamine of 301.02 mg/kg, cadaverine of 29.78 mg/kg, tyramine of 199.21 mg/kg, putrescine of 183.00 mg/kg, and tyramine of 13.01 mg/kg. This strongly suggests that it is necessary to monitor the biogenic amine contents of commercial salted and fermented sand lance sauces carefully to ensure consumer health.


Ammodytes personatus;Fish sauces;Biogenic amines;Histamine;Fermented fish food


  1. AOAC. 1995. Official Methods of Analysis. 16th ed. Association of Official Analytical Chemists, Washington DC., U.S.A., 69-74.
  2. APHA (American Public Health Association). 1984. Laboratory Procedures for the Examination of Sea Water and Shellfish. 5th ed. APHA, Washington DC, U.S.A.
  3. Cho TY, Han GH, Bahn KN, Son YW, Jang MR, Lee CH, Kim SH, Kim DB and Kim SB. 2006. Evaluation of biogenic amines in Korea commercial fermented foods. Korean J Food Sci Technol 38, 730-737.
  4. Cho YJ, Im YS, Lee KW, Kim GB and Chol YJ. 1999. Quality investigation of commercial northern sand lance, Ammodytes personatus sauces. J Korean Fish Soc 32, 612-617.
  5. Hungerford JM. 2010. Scombroid poisoning: a review. Toxicon 56, 231-243.
  6. Joosten HM and Olieman C. 1986. Determination of biogenic amines in cheese and some other food products by highperformance liquid chromatography in combination with thermo-sensitized reaction detection. J Chromatogr 356, 311-319.
  7. KMFDS (Korea Ministry of Food and Drug Safety). 2015. 2015 Korean Food Code. KMFDS, Cheongju, Korea. Retrieved from on 23 September.
  8. Kim BK, Kim YH, Lee HH, Cho YJ, Kim DS, Oh SM and Shim KB. 2011. Comparison of the chemical compositions and biogenic amine contents of salt fermented fish sauces produced in Korea to evaluate the quality characteristics. J Fish Mar Edu 23, 607-614.
  9. Kohara T. 1982. Handbook of food analysis. Kenpakusha, Tokyo, Japan, 51-55.
  10. LeeYS, Homma S and Aida K. 1997. Characterization of melanoidin in soy sauce and fish sauce by electrofocusing and high performance gel permeation chromatography. Nippon Shokuhin Kogyo Gakkaishi 34, 313-319.
  11. Lovenberg W. 1973. Some vaso- and psychoactive substances in food: amines stimulates depressants and hallucinogens. In Toxicants Occurring Naturally in Foods. National Academy of Science. Washington. DC, U.S.A.
  12. Ministry of Social Welfare of Japan. 1960. Guide to Experiment of Sanitary Infection. III. Volatile basic nitrogen. Kenpakusha, Tokyo, Japan, 30-32.
  13. Moon JS, Kim YJ, Jang KI, Cho KJ, Yang SJ, Yoon GM, Kim SY and Han NS. 2010. Analysis of biogenic amines in fermented fish products consumed in Korea. Food Sci Biotechnol 19, 1689-1692.
  14. NFQS (National Fishery Products Quality Management Service). 2013. Retrieved from on 23 September.
  15. Shalaby AR. 1996. Significance of biogenic amines to food safety and human health. Food Res Int 29, 675-690.
  16. Son KT, Lach T, Jung Y, Kang SK, Eom SH, Lee DS, Lee MS and Kim YM. 2014. Food hazard analysis during driedlaver processing. Fish Aquat Sci 17, 197-201.
  17. Taylor SL. 1986. Histamine food poisoning: toxicology and clinical aspects. Crit Rev Toxicol 17, 91-128.
  18. Ten Brink B, Damink C, Joosten HMLJ, Huis in't Veld JHJ. 1990. Occurrence and formation of biologically active amines in foods. Int J Food Microbiol 11, 73-84.
  19. Van Boekel MAJS. 1998. Developments in technologies for food production. In: Innovation of food production systems. W.M.F. Jongen and M.T.G. Meulenberg (Eds.). Wageningen Academic Publishers. Wageningen, Netherlands, 87-116.
  20. Wanakhachornkrai, P. and Lertsiri, S. 2003. Comparison of determination method for volatile compounds in Thai soy sauce. Food Chem 83, 619-629.
  21. Zaman MZ, Abu Baker F, Jinap S and Bakar J. 2011. Novel starter cultures to inhibit biogenic amines accumulation during fish sauce fermentation. Int J Food Microbiol 145, 84-91.