Establishment of an Agrobacterium-mediated Inoculation System for Cucumber Green Mottle Mosaic Virus



Kang, Minji;Seo, Jang-Kyun;Song, Dami;Choi, Hong-Soo;Kim, Kook-Hyung

  • 투고 : 2015.06.26
  • 심사 : 2015.07.23
  • 발행 : 2015.12.01


The infectious full-length cDNA clones of Cucumber green mottle mosaic virus (CGMMV) isolates KW and KOM, which were isolated from watermelon and oriental melon, respectively, were constructed under the control of the cauliflower mosaic virus 35S promoter. We successfully inoculated Nicotiana benthamiana with the cloned CGMMV isolates KW and KOM by Agrobacterium-mediated infiltration. Virulence and symptomatic characteristics of the cloned CGMMV isolates KW and KOM were tested on several indicator plants. No obvious differences between two cloned isolates in disease development were observed on the tested indicator plants. We also determined full genome sequences of the cloned CGMMV isolates KW and KOM. Sequence comparison revealed that only four amino acids (at positions 228, 699, 1212, and 1238 of the replicase protein region) differ between the cloned isolates KW and KOM. A previous study reported that the isolate KOM could not infect Chenopodium amaranticolor, but the cloned KOM induced chlorotic spots on the inoculated leaves. When compared with the previously reported sequence of the original KOM isolate, the cloned KOM contained one amino acid mutation (Ala to Thr) at position 228 of the replicase protein, suggesting that this mutation might be responsible for induction of chlorotic spots on the inoculated leaves of C. amaranticolor.


agroinfiltration;CGMMV;infectious clone


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피인용 문헌

  1. 1. Complete nucleotide sequences and construction of full-length infectious cDNA clones of cucumber green mottle mosaic virus (CGMMV) in a versatile newly developed binary vector including both 35S and T7 promoters vol.53, pp.2, 2017, doi:10.5423/PPJ.NT.06.2015.0123
  2. 2. Exploring Different Mutations at a Single Amino Acid Position of Cucumber green mottle mosaic virus Replicase to Attain Stable Symptom Attenuation vol.107, pp.9, 2017, doi:10.5423/PPJ.NT.06.2015.0123
  3. 3. Recombination-based generation of the agroinfectious clones of Peanut stunt virus vol.237, 2016, doi:10.5423/PPJ.NT.06.2015.0123


연구 과제 주관 기관 : Rural Development Administration, Ministry for Food, Agriculture, Forestry and Fisheries