Loop-mediated Isothermal Amplification Assay to Rapidly Detect Wheat Streak Mosaic Virus in Quarantined Plants

Lee, Siwon;Kim, Jin-Ho;Choi, Ji-Young;Jang, Won-Cheoul

  • Received : 2015.06.12
  • Accepted : 2015.08.12
  • Published : 2015.12.01


We developed a loop-mediated isothermal amplification (LAMP) method to rapidly diagnose Wheat streak mosaic virus (WSMV) during quarantine inspections of imported wheat, corn, oats, and millet. The LAMP method was developed as a plant quarantine inspection method for the first time, and its simplicity, quickness, specificity and sensitivity were verified compared to current reverse transcription-polymerase chain reaction (RT-PCR) and nested PCR quarantine methods. We were able to quickly screen for WSMV at quarantine sites with many test samples; thus, this method is expected to contribute to plant quarantine inspections.


LAMP;loop-mediated isothermal amplification;quarantine;wheat streak mosaic virus;WSMV


  1. Ahn, Y. C., Nam, Y. H., Park, S. N., Cho, M. H., Seo, J. W., Yoon, I. K., Park, Y. H. and Jang, W. C. 2008. Detection of Mycobacterium tuberculosis by loop-mediated isothermal amplification assay. J. Korean Chem. Soc. 52:273-280.
  2. Animal, Plant and Fisheries Quarantine and Inspection Agency. 2013. List of plant quarantine viruses in Korea newly revised in 2013. Res. Plant Dis. 19:67-75.
  3. Cho, M. H., Jang, W. C. and Choi, J. G. 2013. Detection for methicillin resistant Staphylococcus aureus in using bio-chip based loop mediated isothermal amplification assay. J. Korean Chem. Soc. 57:81-87.
  4. French, R. and Robertson, N. L. 1994. An RT-PCR method for the detection of the wheat streak mosaic virus. J. Virol. Methods 49:93-100.
  5. Lee, S. 2013. A study of molecular biological detection methods for seed-transmitted viruses in quarantine. Ph.D. thesis. Dankook University, Cheonan, Chungcheongnam-do, Korea.
  6. Lee, S., Cha, M., Kim, S. M., Heo, N. Y., Shin, Y. G. and Lee, S. H. 2014. Development of nucleotide primers for diagnostic RT-PCR and nested PCR detection of three seed-transmitted viruses (CRLV, SpLV and WClMV) in quarantine. J. Agric. Life Sci. 48:75-83.
  7. Lee, S., Kang, E. H., Chu, Y. M., Shin, Y. G. and Ahn, T. Y. 2013a. Development of PCR diagnosis system for plant quarantine seed-borne Wheat streak mosaic virus. Korean J. Microbiol. 49:112-117.
  8. Lee, S., Kang, E. H., Heo, N. Y., Kim, S. M., Kim, Y. J. and Shin, Y. G. 2013b. Detection of Carnation necrotic fleck vrus and Carnation ringspot virus using RT-PCR. Res. Plant Dis. 19:36-44.
  9. Lee, S., Kang, E. H., Shin, Y. G. and Lee, S. H. 2013c. Development of RT-PCR and nested PCR for detection of four quarantine plant viruses belonging to Nepovirus. Res. Plant Dis. 19:220-225.
  10. Lee, S., Lee, J. Y., Shin, Y. G., Lee, S. H. and Ahn, T. Y. 2015. Development and verification of nested PCR assay for detection of Tobacco rattle virus in plant quarantine. J. Bacteriol. Virol. 45:54-61.
  11. Lee, S. and Shin, Y. G. 2014. Development and practical use of RTPCR for seed-transmitted Prune dwarf virus in quarantine. Plant Pathol. J. 30:178-182.
  12. Shin, Y. G. and Rho, J. Y. 2014. Development of a PCR diagnostic system for Iris yellow spot tospovirus in quarantine. Plant Pathol. J. 30:440-444.

Cited by

  1. Development of rapid and highly sensitive detection of Bean common mosaic necrosis virus in leguminous crops using loop-mediated isothermal amplification assay vol.249, 2017,
  2. Flap endonuclease-initiated enzymatic repairing amplification for ultrasensitive detection of target nucleic acids vol.11, pp.8, 2019,


Supported by : National Research Foundation of Korea (NRF)